ChIP was peformed with H3K4me3 antibody, 17 pg of DNA (subsequently amplified), ChIP'd from 10,000 cells and 35 pg of control DNA (subsequently amplified), ChIP'd from 100,000 cells. The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina platform with the True MicroAmplification kit. The library was analyzed on an Illumina Genome Analyzer. Cluster generation and sequencing were performed using manufacturer instructions.
Matched by Broad Institute dataset
Unmatched by Broad Institute dataset
We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.
ChIP efficiency on 10 000 cells. ChIP was performed on human Hela cells using the Diagenode antibodies H3K4me3 (Cat No. pAb-003-050), H3K27ac (pAb-174-050), H3K9me3 (pAb-056-050) and H3K27me3 (pAb-069-050). Sheared chromatin from 10 000 cells and 0.1 µg (H3K27ac), 0.25 µg (H3K4me3 and H3K27me3) or 0.5 µg (H3K9me3) of the antibody were used per IP. Corresponding amount of IgG was used as control. Quantitative PCR was performed with primers for corresponding positive and negative loci. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
