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<li>
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<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
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<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
<p style="text-align: justify;"></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
<p><span style="font-weight: 400;">Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). </span></p>
<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
<ul>
<li><span style="font-weight: 400;"><strong>Pure exosome capture</strong> - zero contamination from vesicles or aggregates</span></li>
<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
</ul>
<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
<area shape="rect" coords="920,1025,1140,1090" href="../p/captev-serum-plasma-10ml" alt="CaptEV serum plasma" />
<area shape="rect" coords="875,1100,1175,1165" href="../p/evcleaner-1-pc" alt="EVCleaner" />
<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
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<ul>
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<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
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<li>
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<p></p>
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<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
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<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
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<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p></p>
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<li>
<h6>Validation by electron microscopy </h6>
</li>
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<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
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<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
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<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
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<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
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<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
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<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
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<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
<area shape="rect" coords="920,1025,1140,1090" href="../p/captev-serum-plasma-10ml" alt="CaptEV serum plasma" />
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<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
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<p></p>
<h6>Advantages of CaptEV :</h6>
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<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
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<h4>Validation Data</h4>
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<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
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<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
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<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
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<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
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<li>Validation by Flow Cytometry</li>
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<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
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<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
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<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
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<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
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<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
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<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p></p>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
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<h6 style="height:60px">EVCleaner</h6>
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<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
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<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
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<p></p>
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<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
<p><span style="font-weight: 400;">Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). </span></p>
<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
<ul>
<li><span style="font-weight: 400;"><strong>Pure exosome capture</strong> - zero contamination from vesicles or aggregates</span></li>
<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
</ul>
<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
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<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
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<h6>Advantages of CaptEV :</h6>
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<h4>Validation Data</h4>
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<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
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<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
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<li>Validation by electron microscopy</li>
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<div class="row">
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'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
'id' => '2294',
'product_id' => '2856',
'document_id' => '938'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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