Protocols

Protocols

Download RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit
Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disrup...

Download Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus
Standard procedure to shear Chromatin from Yeast.

Download Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL
For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respec...

Download AMPure® XP Size Selection - IP-Star® Compact -
The “AMPure_XP_Size_Selection” protocol on the IP-Star® is using the standard “Agencourt® AMPure® XP Beads” from Agencourt®. It allows you to perform size se...

Download ChIP from tissues with the LowCell# ChIP kit
In theory, you could use 30 mg of tissue per antibody per ChIP. You will start by optimizing conditions for the type of tissue you work with. The exact amount of tissue to start with depends on the...

Download DNA Shearing for Bioruptor® Standard and Plus
For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-pack 0.5) and the corresponding Bioruptor® 0.5 ml Microtubes for DNA Shearing (Cat. No. WA-004-05...

Download Activity Test - Procedure for immunoprecipitation of native chromatin proteins
Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate&rdquo...

Download Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor^® Pico
Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in cells and tissues. The assay can be cumbersome and the success is highly dependent on the quality of ...

Download Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Effici...

Download TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a nat...

Download TruSeq™ ChIP Sample Preparation - IP-Star® Compact
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ ChIP Sample Preparation” kit and reagents from Illumina®. It provides flex...

Download TruSeq™ Stranded RNA Sample Preparation - IP-Star® Compact
The “TruSeq Stranded RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ Stranded Total RNA Sample Preparation” kit or “TruSeq™ Str...

Download iDeal Library Preparation kit - IP-Star® compact
The “iDeal Library Preparation” protocol on the IP-Star® is using the standard “Ideal Library Preparation kit” and reagents from Diagenode. The Ideal Library Preparation...

Download TruSeq™ RNA Sample Preparation - IP-Star Compact
The “TruSeq RNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ RNA Sample Preparation” kit v2 and reagents from Illumina®. It provides fle...

Download AMPure® XP Size Selection - IP-Star Compact
The “AMPure XP Size Selection” protocol on the IP-Star® is using the standard “Agencourt® AMPure® XP Beads” from Agencourt®. It allows you to perform size se...

Download TruSeq™ DNA PCR-Free Sample Preparation - IP-Star Compact
The “TruSeq™ DNA PCR-Free SamplePrep” protocol on the IP-Star® is using the standard “TruSeq™ DNA PCR-Free Sample Preparation” kit and reagents from Illumina...

Download NEBNext® ChIP-Seq Library Prep for Illumina - IP-Star Compact
The “NEBNext ChIP-Seq Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit...

Download MicroPlex Library Preparation kit™ v2 - IP-Star Compact
The “MicroPlex Library Preparation” protocol on the IP-Star® is using the standard “MicroPlex Library Preparation™ kit” and reagents from Diagenode. The MicroPlex ...

Download Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene, or a mineral oil-based method which can be time consuming and messy. Claire Josse and colleagues f...

Download Mass Spectrometry – Using the Bioruptor® to lyse C. Elegans for biochemical and proteomics analysis
Prasad Kasturi and F Ulrich Hartl from the Department of Cellular Biochemistry at the Max Planck Institute for Biochemistry, Martinsried, Germany are using the Bioruptor® for their research usi...

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