Methyltransferase Inhibition Enables Tgf Driven Induction of and in Cancer Cells. Published: January, 2023 Abstract:
deletion or silencing is common across human cancer, reinforcing the general importance of bypassing its tumor suppression in cancer formation or progression. In rhabdomyosarcoma (RMS) and neuroblastoma, two common childhood cancers, the three tra... Read more »
Transgenic mice for in vivo epigenome editing with CRISPR-based systems Published: March, 2021 Abstract:
The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chr... Read more »
DNA Methylation Editing by CRISPR-guided Excision of 5-Methylcytosine. Published: February, 2020 Abstract:
Tools for actively targeted DNA demethylation are required to increase our knowledge about regulation and specific functions of this important epigenetic modification. DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosin... Read more »
DAPK1 loss triggers tumor invasion in colorectal tumor cells. Published: November, 2019 Abstract:
Colorectal cancer (CRC) is one of the leading cancer-related causes of death worldwide. Despite the improvement of surgical and chemotherapeutic treatments, as of yet, the disease has not been overcome due to metastasis to distant organs. Hence, i... Read more »
Guidelines for optimized gene knockout using CRISPR/Cas9 Published: May, 2019 Abstract:
CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a genome-editing experiment can be substantially impacted by... Read more »
Long-term evaluation of AAV-CRISPR genome editing for Duchenne muscular dystrophy. Published: March, 2019 Abstract:
Duchenne muscular dystrophy (DMD) is a monogenic disorder and a candidate for therapeutic genome editing. There have been several recent reports of genome editing in preclinical models of Duchenne muscular dystrophy, however, the long-term persist... Read more »
One-step generation of modular CAR-T cells with AAV-Cpf1. Published: March, 2019 Abstract:
Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced fea... Read more »
zHSF1 modulates zper2 expression in zebrafish embryos Published: March, 2018 Abstract:
HSF1 is a transcription factor that plays a key role in circadian resetting by temperature. We have used zebrafish embryos to decipher the roles of zHsf1, heat and light on zper2 transcription in vivo. Our results show that heat shock (HS) stimula... Read more »
A self-inactivating system for AAV-mediated in vivo base editing Abstract:
DNA base editors have been harnessed as an exciting therapeutic platform for human diseases and are rapidly progressing into human clinical trials. However, persistent expression of base editors delivered via adeno-associated virus (AAV) poses con... Read more »
