Prediction of brain metastasis development with DNA methylation signatures Published: October, 2024 Abstract:
Brain metastases (BMs) are the most common and among the deadliest brain tumors. Currently, there are no reliable predictors of BM development from primary cancer, which limits early intervention. Lung adenocarcinoma (LUAD) is the most common BM s... Read more »
Prostate cancer detection through unbiased capture of methylated cell-free DNA Published: June, 2024 Abstract:
Prostate cancer screening using prostate-specific antigen (PSA) has been shown to reduce mortality but with substantial overdiagnosis, leading to unnecessary biopsies. The identification of a highly specific biomarker using liquid biopsies, repres... Read more »
In vitro production of cat-restricted Toxoplasma pre-sexual stages Published: December, 2023 Abstract:
Sexual reproduction of Toxoplasma gondii, confined to the felid gut, remains largely uncharted owing to ethical concerns regarding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during i... Read more »
Comprehensive characterization of the epigenetic landscape in Multiple Myeloma Published: January, 2022 Abstract:
Background: Human multiple myeloma (MM) cell lines (HMCLs) have been widely used to understand themolecular processes that drive MM biology. Epigenetic modifications are involved in MM development,progression, and drug resistance. A comprehensive ... Read more »
Detection of renal cell carcinoma using plasma and urine cell-free DNA methylomes. Published: June, 2020 Abstract:
Improving early cancer detection has the potential to substantially reduce cancer-related mortality. Cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq) is a highly sensitive assay capable of detecting early-s... Read more »
Ikaros antagonizes DNA binding by STAT5 in pre-B cells. Published: January, 2020 Abstract:
The IKZF1 gene, which encodes the Ikaros transcription factor, is frequently deleted or mutated in patients with B-cell precursor acute lymphoblastic leukemias that express oncogenes, like BCR-ABL, which activate the JAK-STAT5 pathway. Ikaros func... Read more »
EZH2 as a novel therapeutic target for atrial fibrosis and atrial fibrillation. Published: August, 2019 Abstract:
Angiotensin II (Ang-II)-induced fibroblast differentiation plays an important role in the development of atrial fibrosis and atrial fibrillation (AF). Here, we show that the expression of the histone methyltransferase enhancer of zeste homolog 2 (... Read more »
Sensitive tumour detection and classification using plasma cell-free DNA methylomes. Published: November, 2018 Abstract:
The use of liquid biopsies for cancer detection and management is rapidly gaining prominence. Current methods for the detection of circulating tumour DNA involve sequencing somatic mutations using cell-free DNA, but the sensitivity of these method... Read more »
Epigenetic regulation of brain region-specific microglia clearance activity. Published: August, 2018 Abstract:
The rapid elimination of dying neurons and nonfunctional synapses in the brain is carried out by microglia, the resident myeloid cells of the brain. Here we show that microglia clearance activity in the adult brain is regionally regulated and depe... Read more »
p27 regulates alpha-synuclein expression. Published: March, 2018 Abstract:
Alpha-synuclein (α-SYN) is the main component of anomalous protein aggregates (Lewy bodies) that play a crucial role in several neurodegenerative diseases (synucleinopathies) like Parkinson's disease and multiple system atrophy. However, the... Read more »
Data on novel DNA methylation changes induced by valproic acid in human hepatocytes Published: November, 2017 Abstract:
Valproic acid (VPA) is a widely prescribed antiepileptic drug in the world. Despite its pharmacological importance, it may cause liver toxicity and steatosis. However the exact mechanism of the steatosis formation is unknown. The data presented in... Read more »
High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing Published: September, 2017 Abstract:
Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. This metho... Read more »
Epigenome profiling and editing of neocortical progenitor cells during development Published: September, 2017 Abstract:
The generation of neocortical neurons from neural progenitor cells (NPCs) is primarily controlled by transcription factors binding to DNA in the context of chromatin. To understand the complex layer of regulation that orchestrates different NPC ty... Read more »
sgs1: a neomorphic nac52 allele impairing PTGS through SGS3 down-regulation Published: May, 2017 Abstract:
Post-transcriptional gene silencing (PTGS) is a defense mechanism that targets invading nucleic acids from endogenous (transposons) or exogenous (pathogens, transgenes) sources. Genetic screens based on the reactivation of silenced transgenes have... Read more »
Genomic Characterization of Metformin Hepatic Response Published: November, 2016 Abstract:
Metformin is used as a first-line therapy for type 2 diabetes (T2D) and prescribed for numerous other diseases. However, its mechanism of action in the liver has yet to be characterized in a systematic manner. To comprehensively identify genes and... Read more »
Targeting the MLL complex in castration-resistant prostate cancer. Published: April, 2015 Abstract:
Resistance to androgen deprivation therapies and increased androgen receptor (AR) activity are major drivers of castration-resistant prostate cancer (CRPC). Although prior work has focused on targeting AR directly, co-activators of AR signaling, w... Read more »
The cohesin complex regulates immunoglobulin class switch recombination. Published: November, 2013 Abstract:
Immunoglobulin (Ig) class switch recombination (CSR) is initiated by the transcription-coupled recruitment of activation-induced cytidine deaminase (AID) to switch regions and by the subsequent generation of double-stranded DNA breaks (DSBs). Thes... Read more »
Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator. Published: January, 2013 Abstract:
Because regulation of its activity is instrumental either to support cell proliferation and growth or to promote cell death, the universal myo-inositol phosphate synthase (MIPS), responsible for myo-inositol biosynthesis, is a critical enzyme of p... Read more »
