Development of PCR screening assays focused on genecoding sequences for GMO detection Published: September, 2018 Abstract:
For the detection of genetically modifed organisms (GMO), screening is the frst step used to determine if a GMO or its derived products are present in food or feed. If the result is positive, additional tests must be done to identify and, where ne... Read more »
Detection of Hermetia illucens by real-time PCR Published: January, 2018 Abstract:
Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins ob... Read more »
The LXR-Idol Axis Differentially Regulates Plasma LDL Levels in Primates and Mice. Published: November, 2014 Abstract:
The LXR-regulated E3 ubiquitin ligase IDOL controls LDLR receptor stability independent of SREBP and PCSK9, but its relevance to plasma lipid levels is unknown. Here we demonstrate that the effects of the LXR-IDOL axis are both tissue and species ... Read more »
The Impact of Breastfeeding on Nasopharyngeal Microbial Communities in Infants. Published: June, 2014 Abstract:
Rationale. Breastfeeding elicits significant protection against respiratory tract infections in infancy. Modulation of respiratory microbiota might be part of the natural mechanisms of protection against respiratory diseases induced by breastfeedi... Read more »
A high-throughput method for GMO multi-detection using a microfluidic dynamic array. Published: February, 2014 Abstract:
The ever-increasing production of genetically modified crops generates a demand for high-throughput DNA-based methods for the enforcement of genetically modified organisms (GMO) labelling requirements. The application of standard real-time PCR wil... Read more »
Reprogramming of lysosomal gene expression by interleukin-4 and Stat6 Published: December, 2013 Abstract:
Background Lysosomes play important roles in multiple aspects of physiology, but the problem of how the transcription of lysosomal genes is coordinated remains incompletely understood. The goal of this study was to illuminate the physiological c... Read more »
Quantification of biomass and cell motion in human pluripotent stem cell colonies. Published: August, 2013 Abstract:
Somatic cell reprogramming to pluripotency requires an immediate increase in cell proliferation and reduction in cell size. It is unknown whether proliferation and biomass controls are similarly coordinated with early events during the differentia... Read more »
Autologous platelet-poor plasma decreases the bronchial stump necrosis in rat Published: January, 2013 Abstract:
Background: Necrosis of the bronchial stump is a very important trigger for bronchopleural fistula. The administration of local autologous platelet-poor plasma (PPP) could protect the bronchial stump. Materials and methods: Left pneumonectomy was ... Read more »
Simulation of between Repeat Variability in Real Time PCR Reactions. Published: November, 2012 Abstract:
While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process. Besides influences of... Read more »
Vestigial-like 3 (Vgll3) is an Inhibitor of Adipocyte Differentiation. Published: November, 2012 Abstract:
Abstract Abstract Adipose differentiation is a complex process controlled by a network of transcription factors and co-regulators. We compared the global gene expression patterns of adipogenic and non-adipogenic clones of 3T3-F442A pre-adipocytes... Read more »
Divergent antiviral effects of bioflavonoids on the hepatitis C virus life cycle. Published: September, 2012 Abstract:
We have previously demonstrated that quercetin, a bioflavonoid, blocks hepatitis C virus (HCV) proliferation by inhibiting NS5A-driven internal ribosomal entry site (IRES)-mediated translation of the viral genome. Here, we investigate the mechanis... Read more »
HOXB7 promotes invasion and predicts survival in pancreatic adenocarcinoma. Published: August, 2012 Abstract:
BACKGROUND: The homeobox gene HOXB7 is overexpressed across a range of cancers and promotes tumorigenesis through varying effects on proliferation, survival, invasion, and angiogenesis. Although published microarray data suggest HOXB7 is overexpr... Read more »
TLE3 is a dual-function transcriptional coregulator of adipogenesis. Published: April, 2011 Abstract:
PPARγ and Wnt signaling are central positive and negative regulators of adipogenesis, respectively. Here we identify the groucho family member TLE3 as a transcriptional integrator of the PPARγ and Wnt pathways. TLE3 is a direct target of PPARγ tha... Read more »
Increased efficacy for in-house validation of real-time PCR GMO detection methods. Published: March, 2010 Abstract:
To improve the efficacy of the in-house validation of GMO detection methods (DNA isolation and real-time PCR, polymerase chain reaction), a study was performed to gain insight in the contribution of the different steps of the GMO detection method ... Read more »
Epigenetic engineering of ribosomal RNA genes enhances protein production. Published: January, 2009 Abstract:
Selection of mammalian high-producer cell lines remains a major challenge for the biopharmaceutical manufacturing industry. Ribosomal RNA (rRNA) genes encode the major component of the ribosome but many rRNA gene copies are not transcribed due to ... Read more »
