Chromatin immunoprecipitation followed by next generation sequencing (ChIP-Seq) is a powerful technique for generating genome-wide profiles of epigenetic modifications and transcription factor binding sites. “Manual ChIP assays” use labor-intensive, complex protocols that can introduce experimental variability and limit day-to-day and lab-to-lab reproducibility. The IP-Star Automation System reduces variability and eliminates the time consuming steps of manually performed ChIP assays.