Analysis of histone modifications in different cells within different selected regions of the genome is important to associate gene expression status with an exact distribution of epigenetic marks.
Chromatin Immunoprecipitation (ChIP) is a technique allowing the analysis of the histone modifications associated with specific genomic regions in the context of intact cells(1). ChIP is then used to connect epigenetic marks to intergenic regions, active coding regions and/or silenced coding regions. The main steps of the ChIP technique are cell fixation, chromatin shearing, immunoselection, immunoprecipitation (IP), and analysis of the immunoprecipitated (IP’d) DNA.
