Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'product' => array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Testimonial' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = '15 ml Bioruptor® Tubes & sonication beads'
$meta_title = '15 ml Bioruptor® Tubes & sonication beads'
$product = array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '40',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Pico accessories',
'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-pico-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico',
'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode',
'modified' => '2018-02-14 16:24:24',
'created' => '2015-06-12 10:01:53',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '782',
'name' => '15 ml Bioruptor® Tubes & sonication beads',
'description' => '<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The 15 ml Bioruptor</span><span><sup>®</sup> </span><span>Tubes & sonication beads for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor</span><sup><span>® </span></sup><span>Pico (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_15ml_tube_sonication_beads.pdf',
'slug' => 'datasheet-15ml-tube-sonication-beads',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-07-24 13:29:43',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '125',
'name' => 'product/shearing_technologies/C01020031_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4793',
'name' => 'Targeting lymphoid-derived IL-17 signaling to delay skin aging.',
'authors' => 'Paloma S. et al.',
'description' => '<p><span>Skin aging is characterized by structural and functional changes that contribute to age-associated frailty. This probably depends on synergy between alterations in the local niche and stem cell-intrinsic changes, underscored by proinflammatory microenvironments that drive pleotropic changes. The nature of these age-associated inflammatory cues, or how they affect tissue aging, is unknown. Based on single-cell RNA sequencing of the dermal compartment of mouse skin, we show a skew towards an IL-17-expressing phenotype of T helper cells, γδ T cells and innate lymphoid cells in aged skin. Importantly, in vivo blockade of IL-17 signaling during aging reduces the proinflammatory state of the skin, delaying the appearance of age-related traits. Mechanistically, aberrant IL-17 signals through NF-κB in epidermal cells to impair homeostatic functions while promoting an inflammatory state. Our results indicate that aged skin shows signs of chronic inflammation and that increased IL-17 signaling could be targeted to prevent age-associated skin ailments.</span></p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37291218',
'doi' => '10.1038/s43587-023-00431-z',
'modified' => '2023-06-14 15:56:56',
'created' => '2023-06-13 21:11:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4141',
'name' => 'Transgenic mice for in vivo epigenome editing with CRISPR-based systems',
'authors' => 'Gemberling, M. et al.',
'description' => '<p>The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chromatin-modifying domains to the nuclease-deactivated form of Cas9 (dCas9) has enabled targeted gene activation or repression in both cultured cells and in vivo in animal models. However, delivery of the large dCas9 fusion proteins to target cell types and tissues is an obstacle to widespread adoption of these tools for in vivo studies. Here we describe the generation and validation of two conditional transgenic mouse lines for targeted gene regulation, Rosa26:LSL-dCas9-p300 for gene activation and Rosa26:LSL-dCas9-KRAB for gene repression. Using the dCas9p300 and dCas9KRAB transgenic mice we demonstrate activation or repression of genes in both the brain and liver in vivo, and T cells and fibroblasts ex vivo. We show gene regulation and targeted epigenetic modification with gRNAs targeting either transcriptional start sites (TSS) or distal enhancer elements, as well as corresponding changes to downstream phenotypes. These mouse lines are convenient and valuable tools for facile, temporally controlled, and tissue-restricted epigenome editing and manipulation of gene expression in vivo.</p>',
'date' => '2021-03-01',
'pmid' => 'https://doi.org/10.1101%2F2021.03.08.434430',
'doi' => '10.1101/2021.03.08.434430',
'modified' => '2021-12-13 09:23:10',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4144',
'name' => 'REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR',
'authors' => 'Suh, J. L. et al.',
'description' => '<p>Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their misregulation associated with human disease including cancer, CBX proteins are attractive targets for small molecule chemical probe development. Here, we have used a quantitative and target-specific cellular assay to discover a potent positive allosteric modulator (PAM) of CBX8. The PAM activity of UNC7040 antagonizes H3K27me3 binding by CBX8 while increasing interactions with nucleic acids and participation in variant PRC1. We show that treatment with UNC7040 leads to efficient PRC1 chromatin eviction, loss of silencing and reduced proliferation across different cancer cell lines. Our discovery and characterization of UNC7040 not only revealed the most cellularly potent CBX8-specific chemical probe to date, but also corroborates a mechanism of polycomb regulation by non-histone lysine methylated interaction partners.</p>',
'date' => '2021-02-01',
'pmid' => 'https://doi.org/10.1101%2F2021.02.23.432388',
'doi' => '10.1101/2021.02.23.432388',
'modified' => '2021-12-13 09:35:04',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3961',
'name' => 'Estrogen induces dynamic ERα and RING1B recruitment to control gene and enhancer activities in luminal breast cancer',
'authors' => 'Zhang Yusheng, Chan Ho Lam, Garcia-Martinez Liliana, Karl Daniel L., Weich Natalia, Slingerland Joyce M., Verdun Ramiro E., Morey Lluis',
'description' => '<p>RING1B, a core Polycomb repressive complex 1 subunit, is a histone H2A ubiquitin ligase essential for development. RING1B is overexpressed in patients with luminal breast cancer (BC) and recruited to actively transcribed genes and enhancers co-occupied by the estrogen receptor (ER). Whether ER-induced transcriptional programs are mediated by RING1B is not understood. We show that prolonged estrogen administration induces transcriptional output and chromatin landscape fluctuations. RING1B loss impairs full estrogen-mediated gene expression and chromatin accessibility for key BC transcription factors. These effects were mediated, in part, by RING1B enzymatic activity and nucleosome binding functions. RING1B is recruited in a cyclic manner to ER, FOXA1, and GRHL2 cobound sites and regulates estrogen-induced enhancers and ER recruitment. Last, ChIP exo revealed multiple binding events of these factors at single-nucleotide resolution, including RING1B occupancy approximately 10 base pairs around ER bound sites. We propose RING1B as a key regulator of the dynamic, liganded-ER transcriptional regulatory circuit in luminal BC.</p>',
'date' => '2020-06-12',
'pmid' => 'https://advances.sciencemag.org/content/6/23/eaaz7249.full',
'doi' => '10.1126/sciadv.aaz7249',
'modified' => '2020-08-12 09:48:21',
'created' => '2020-08-10 12:12:25',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
[maximum depth reached]
)
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3288',
'name' => 'Sonication beads SDS US en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3286',
'name' => 'Sonication beads SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3281',
'name' => 'Sonication beads SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3285',
'name' => 'Sonication beads SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3284',
'name' => 'Sonication beads SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3283',
'name' => 'Sonication beads SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3287',
'name' => 'Sonication beads SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = '<blockquote><p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p><cite>Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford</cite></blockquote>
'
$featured_testimonials = ''
$testimonial = array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
'id' => '52',
'product_id' => '1875',
'testimonial_id' => '37'
)
)
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200016_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200016</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml sonication accessories for Picoruptor</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '1763',
'product_id' => '1875',
'related_id' => '1796'
),
'Image' => array(
(int) 0 => array(
'id' => '116',
'name' => 'product/shearing_technologies/B01200016_tube_holder.jpg',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '44',
'product_id' => '1875',
'protocol_id' => '4'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '736',
'product_id' => '1875',
'document_id' => '791'
)
)
$sds = array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
'id' => '5322',
'product_id' => '1875',
'safety_sheet_id' => '3282'
)
)
$publication = array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
'id' => '2856',
'product_id' => '1875',
'publication_id' => '3406'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30077771" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'product' => array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Testimonial' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = '15 ml Bioruptor® Tubes & sonication beads'
$meta_title = '15 ml Bioruptor® Tubes & sonication beads'
$product = array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '40',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Pico accessories',
'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-pico-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico',
'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode',
'modified' => '2018-02-14 16:24:24',
'created' => '2015-06-12 10:01:53',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '782',
'name' => '15 ml Bioruptor® Tubes & sonication beads',
'description' => '<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The 15 ml Bioruptor</span><span><sup>®</sup> </span><span>Tubes & sonication beads for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor</span><sup><span>® </span></sup><span>Pico (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_15ml_tube_sonication_beads.pdf',
'slug' => 'datasheet-15ml-tube-sonication-beads',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-07-24 13:29:43',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '125',
'name' => 'product/shearing_technologies/C01020031_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4793',
'name' => 'Targeting lymphoid-derived IL-17 signaling to delay skin aging.',
'authors' => 'Paloma S. et al.',
'description' => '<p><span>Skin aging is characterized by structural and functional changes that contribute to age-associated frailty. This probably depends on synergy between alterations in the local niche and stem cell-intrinsic changes, underscored by proinflammatory microenvironments that drive pleotropic changes. The nature of these age-associated inflammatory cues, or how they affect tissue aging, is unknown. Based on single-cell RNA sequencing of the dermal compartment of mouse skin, we show a skew towards an IL-17-expressing phenotype of T helper cells, γδ T cells and innate lymphoid cells in aged skin. Importantly, in vivo blockade of IL-17 signaling during aging reduces the proinflammatory state of the skin, delaying the appearance of age-related traits. Mechanistically, aberrant IL-17 signals through NF-κB in epidermal cells to impair homeostatic functions while promoting an inflammatory state. Our results indicate that aged skin shows signs of chronic inflammation and that increased IL-17 signaling could be targeted to prevent age-associated skin ailments.</span></p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37291218',
'doi' => '10.1038/s43587-023-00431-z',
'modified' => '2023-06-14 15:56:56',
'created' => '2023-06-13 21:11:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4141',
'name' => 'Transgenic mice for in vivo epigenome editing with CRISPR-based systems',
'authors' => 'Gemberling, M. et al.',
'description' => '<p>The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chromatin-modifying domains to the nuclease-deactivated form of Cas9 (dCas9) has enabled targeted gene activation or repression in both cultured cells and in vivo in animal models. However, delivery of the large dCas9 fusion proteins to target cell types and tissues is an obstacle to widespread adoption of these tools for in vivo studies. Here we describe the generation and validation of two conditional transgenic mouse lines for targeted gene regulation, Rosa26:LSL-dCas9-p300 for gene activation and Rosa26:LSL-dCas9-KRAB for gene repression. Using the dCas9p300 and dCas9KRAB transgenic mice we demonstrate activation or repression of genes in both the brain and liver in vivo, and T cells and fibroblasts ex vivo. We show gene regulation and targeted epigenetic modification with gRNAs targeting either transcriptional start sites (TSS) or distal enhancer elements, as well as corresponding changes to downstream phenotypes. These mouse lines are convenient and valuable tools for facile, temporally controlled, and tissue-restricted epigenome editing and manipulation of gene expression in vivo.</p>',
'date' => '2021-03-01',
'pmid' => 'https://doi.org/10.1101%2F2021.03.08.434430',
'doi' => '10.1101/2021.03.08.434430',
'modified' => '2021-12-13 09:23:10',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4144',
'name' => 'REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR',
'authors' => 'Suh, J. L. et al.',
'description' => '<p>Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their misregulation associated with human disease including cancer, CBX proteins are attractive targets for small molecule chemical probe development. Here, we have used a quantitative and target-specific cellular assay to discover a potent positive allosteric modulator (PAM) of CBX8. The PAM activity of UNC7040 antagonizes H3K27me3 binding by CBX8 while increasing interactions with nucleic acids and participation in variant PRC1. We show that treatment with UNC7040 leads to efficient PRC1 chromatin eviction, loss of silencing and reduced proliferation across different cancer cell lines. Our discovery and characterization of UNC7040 not only revealed the most cellularly potent CBX8-specific chemical probe to date, but also corroborates a mechanism of polycomb regulation by non-histone lysine methylated interaction partners.</p>',
'date' => '2021-02-01',
'pmid' => 'https://doi.org/10.1101%2F2021.02.23.432388',
'doi' => '10.1101/2021.02.23.432388',
'modified' => '2021-12-13 09:35:04',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3961',
'name' => 'Estrogen induces dynamic ERα and RING1B recruitment to control gene and enhancer activities in luminal breast cancer',
'authors' => 'Zhang Yusheng, Chan Ho Lam, Garcia-Martinez Liliana, Karl Daniel L., Weich Natalia, Slingerland Joyce M., Verdun Ramiro E., Morey Lluis',
'description' => '<p>RING1B, a core Polycomb repressive complex 1 subunit, is a histone H2A ubiquitin ligase essential for development. RING1B is overexpressed in patients with luminal breast cancer (BC) and recruited to actively transcribed genes and enhancers co-occupied by the estrogen receptor (ER). Whether ER-induced transcriptional programs are mediated by RING1B is not understood. We show that prolonged estrogen administration induces transcriptional output and chromatin landscape fluctuations. RING1B loss impairs full estrogen-mediated gene expression and chromatin accessibility for key BC transcription factors. These effects were mediated, in part, by RING1B enzymatic activity and nucleosome binding functions. RING1B is recruited in a cyclic manner to ER, FOXA1, and GRHL2 cobound sites and regulates estrogen-induced enhancers and ER recruitment. Last, ChIP exo revealed multiple binding events of these factors at single-nucleotide resolution, including RING1B occupancy approximately 10 base pairs around ER bound sites. We propose RING1B as a key regulator of the dynamic, liganded-ER transcriptional regulatory circuit in luminal BC.</p>',
'date' => '2020-06-12',
'pmid' => 'https://advances.sciencemag.org/content/6/23/eaaz7249.full',
'doi' => '10.1126/sciadv.aaz7249',
'modified' => '2020-08-12 09:48:21',
'created' => '2020-08-10 12:12:25',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
[maximum depth reached]
)
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3288',
'name' => 'Sonication beads SDS US en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3286',
'name' => 'Sonication beads SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3281',
'name' => 'Sonication beads SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3285',
'name' => 'Sonication beads SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3284',
'name' => 'Sonication beads SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3283',
'name' => 'Sonication beads SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3287',
'name' => 'Sonication beads SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = '<blockquote><p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p><cite>Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford</cite></blockquote>
'
$featured_testimonials = ''
$testimonial = array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
'id' => '52',
'product_id' => '1875',
'testimonial_id' => '37'
)
)
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200016_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200016</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml sonication accessories for Picoruptor</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '1763',
'product_id' => '1875',
'related_id' => '1796'
),
'Image' => array(
(int) 0 => array(
'id' => '116',
'name' => 'product/shearing_technologies/B01200016_tube_holder.jpg',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '44',
'product_id' => '1875',
'protocol_id' => '4'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '736',
'product_id' => '1875',
'document_id' => '791'
)
)
$sds = array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
'id' => '5322',
'product_id' => '1875',
'safety_sheet_id' => '3282'
)
)
$publication = array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
'id' => '2856',
'product_id' => '1875',
'publication_id' => '3406'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30077771" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'product' => array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Testimonial' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = '15 ml Bioruptor® Tubes & sonication beads'
$meta_title = '15 ml Bioruptor® Tubes & sonication beads'
$product = array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '40',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Pico accessories',
'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-pico-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico',
'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode',
'modified' => '2018-02-14 16:24:24',
'created' => '2015-06-12 10:01:53',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '782',
'name' => '15 ml Bioruptor® Tubes & sonication beads',
'description' => '<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The 15 ml Bioruptor</span><span><sup>®</sup> </span><span>Tubes & sonication beads for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor</span><sup><span>® </span></sup><span>Pico (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_15ml_tube_sonication_beads.pdf',
'slug' => 'datasheet-15ml-tube-sonication-beads',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-07-24 13:29:43',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '125',
'name' => 'product/shearing_technologies/C01020031_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4793',
'name' => 'Targeting lymphoid-derived IL-17 signaling to delay skin aging.',
'authors' => 'Paloma S. et al.',
'description' => '<p><span>Skin aging is characterized by structural and functional changes that contribute to age-associated frailty. This probably depends on synergy between alterations in the local niche and stem cell-intrinsic changes, underscored by proinflammatory microenvironments that drive pleotropic changes. The nature of these age-associated inflammatory cues, or how they affect tissue aging, is unknown. Based on single-cell RNA sequencing of the dermal compartment of mouse skin, we show a skew towards an IL-17-expressing phenotype of T helper cells, γδ T cells and innate lymphoid cells in aged skin. Importantly, in vivo blockade of IL-17 signaling during aging reduces the proinflammatory state of the skin, delaying the appearance of age-related traits. Mechanistically, aberrant IL-17 signals through NF-κB in epidermal cells to impair homeostatic functions while promoting an inflammatory state. Our results indicate that aged skin shows signs of chronic inflammation and that increased IL-17 signaling could be targeted to prevent age-associated skin ailments.</span></p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37291218',
'doi' => '10.1038/s43587-023-00431-z',
'modified' => '2023-06-14 15:56:56',
'created' => '2023-06-13 21:11:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4141',
'name' => 'Transgenic mice for in vivo epigenome editing with CRISPR-based systems',
'authors' => 'Gemberling, M. et al.',
'description' => '<p>The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chromatin-modifying domains to the nuclease-deactivated form of Cas9 (dCas9) has enabled targeted gene activation or repression in both cultured cells and in vivo in animal models. However, delivery of the large dCas9 fusion proteins to target cell types and tissues is an obstacle to widespread adoption of these tools for in vivo studies. Here we describe the generation and validation of two conditional transgenic mouse lines for targeted gene regulation, Rosa26:LSL-dCas9-p300 for gene activation and Rosa26:LSL-dCas9-KRAB for gene repression. Using the dCas9p300 and dCas9KRAB transgenic mice we demonstrate activation or repression of genes in both the brain and liver in vivo, and T cells and fibroblasts ex vivo. We show gene regulation and targeted epigenetic modification with gRNAs targeting either transcriptional start sites (TSS) or distal enhancer elements, as well as corresponding changes to downstream phenotypes. These mouse lines are convenient and valuable tools for facile, temporally controlled, and tissue-restricted epigenome editing and manipulation of gene expression in vivo.</p>',
'date' => '2021-03-01',
'pmid' => 'https://doi.org/10.1101%2F2021.03.08.434430',
'doi' => '10.1101/2021.03.08.434430',
'modified' => '2021-12-13 09:23:10',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4144',
'name' => 'REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR',
'authors' => 'Suh, J. L. et al.',
'description' => '<p>Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their misregulation associated with human disease including cancer, CBX proteins are attractive targets for small molecule chemical probe development. Here, we have used a quantitative and target-specific cellular assay to discover a potent positive allosteric modulator (PAM) of CBX8. The PAM activity of UNC7040 antagonizes H3K27me3 binding by CBX8 while increasing interactions with nucleic acids and participation in variant PRC1. We show that treatment with UNC7040 leads to efficient PRC1 chromatin eviction, loss of silencing and reduced proliferation across different cancer cell lines. Our discovery and characterization of UNC7040 not only revealed the most cellularly potent CBX8-specific chemical probe to date, but also corroborates a mechanism of polycomb regulation by non-histone lysine methylated interaction partners.</p>',
'date' => '2021-02-01',
'pmid' => 'https://doi.org/10.1101%2F2021.02.23.432388',
'doi' => '10.1101/2021.02.23.432388',
'modified' => '2021-12-13 09:35:04',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3961',
'name' => 'Estrogen induces dynamic ERα and RING1B recruitment to control gene and enhancer activities in luminal breast cancer',
'authors' => 'Zhang Yusheng, Chan Ho Lam, Garcia-Martinez Liliana, Karl Daniel L., Weich Natalia, Slingerland Joyce M., Verdun Ramiro E., Morey Lluis',
'description' => '<p>RING1B, a core Polycomb repressive complex 1 subunit, is a histone H2A ubiquitin ligase essential for development. RING1B is overexpressed in patients with luminal breast cancer (BC) and recruited to actively transcribed genes and enhancers co-occupied by the estrogen receptor (ER). Whether ER-induced transcriptional programs are mediated by RING1B is not understood. We show that prolonged estrogen administration induces transcriptional output and chromatin landscape fluctuations. RING1B loss impairs full estrogen-mediated gene expression and chromatin accessibility for key BC transcription factors. These effects were mediated, in part, by RING1B enzymatic activity and nucleosome binding functions. RING1B is recruited in a cyclic manner to ER, FOXA1, and GRHL2 cobound sites and regulates estrogen-induced enhancers and ER recruitment. Last, ChIP exo revealed multiple binding events of these factors at single-nucleotide resolution, including RING1B occupancy approximately 10 base pairs around ER bound sites. We propose RING1B as a key regulator of the dynamic, liganded-ER transcriptional regulatory circuit in luminal BC.</p>',
'date' => '2020-06-12',
'pmid' => 'https://advances.sciencemag.org/content/6/23/eaaz7249.full',
'doi' => '10.1126/sciadv.aaz7249',
'modified' => '2020-08-12 09:48:21',
'created' => '2020-08-10 12:12:25',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
[maximum depth reached]
)
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3288',
'name' => 'Sonication beads SDS US en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3286',
'name' => 'Sonication beads SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3281',
'name' => 'Sonication beads SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3285',
'name' => 'Sonication beads SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3284',
'name' => 'Sonication beads SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3283',
'name' => 'Sonication beads SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3287',
'name' => 'Sonication beads SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = '<blockquote><p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p><cite>Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford</cite></blockquote>
'
$featured_testimonials = ''
$testimonial = array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
'id' => '52',
'product_id' => '1875',
'testimonial_id' => '37'
)
)
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200016_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200016</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml sonication accessories for Picoruptor</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '1763',
'product_id' => '1875',
'related_id' => '1796'
),
'Image' => array(
(int) 0 => array(
'id' => '116',
'name' => 'product/shearing_technologies/B01200016_tube_holder.jpg',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '44',
'product_id' => '1875',
'protocol_id' => '4'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '736',
'product_id' => '1875',
'document_id' => '791'
)
)
$sds = array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
'id' => '5322',
'product_id' => '1875',
'safety_sheet_id' => '3282'
)
)
$publication = array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
'id' => '2856',
'product_id' => '1875',
'publication_id' => '3406'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30077771" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'product' => array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
)
),
'Testimonial' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = '15 ml Bioruptor® Tubes & sonication beads'
$meta_title = '15 ml Bioruptor® Tubes & sonication beads'
$product = array(
'Product' => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Picoruptor Tubes & sonication beads',
'description' => '<p><span>The 15 ml Picoruptor Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(
(int) 0 => array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(
(int) 0 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'slug' => 'chromatin-shearing',
'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator',
'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.',
'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
'modified' => '2017-11-15 10:14:02',
'created' => '2015-03-05 15:56:30',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '40',
'position' => '1',
'parent_id' => '6',
'name' => 'Bioruptor<sup>®</sup> Pico accessories',
'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'bioruptor-pico-accessories',
'cookies_tag_id' => null,
'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator',
'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico',
'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode',
'modified' => '2018-02-14 16:24:24',
'created' => '2015-06-12 10:01:53',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '782',
'name' => '15 ml Bioruptor® Tubes & sonication beads',
'description' => '<div class="page" title="Page 1">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The 15 ml Bioruptor</span><span><sup>®</sup> </span><span>Tubes & sonication beads for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor</span><sup><span>® </span></sup><span>Pico (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor/Datasheet_15ml_tube_sonication_beads.pdf',
'slug' => 'datasheet-15ml-tube-sonication-beads',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-07-24 13:29:43',
'created' => '2015-07-07 11:47:45',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '125',
'name' => 'product/shearing_technologies/C01020031_tube.png',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '4793',
'name' => 'Targeting lymphoid-derived IL-17 signaling to delay skin aging.',
'authors' => 'Paloma S. et al.',
'description' => '<p><span>Skin aging is characterized by structural and functional changes that contribute to age-associated frailty. This probably depends on synergy between alterations in the local niche and stem cell-intrinsic changes, underscored by proinflammatory microenvironments that drive pleotropic changes. The nature of these age-associated inflammatory cues, or how they affect tissue aging, is unknown. Based on single-cell RNA sequencing of the dermal compartment of mouse skin, we show a skew towards an IL-17-expressing phenotype of T helper cells, γδ T cells and innate lymphoid cells in aged skin. Importantly, in vivo blockade of IL-17 signaling during aging reduces the proinflammatory state of the skin, delaying the appearance of age-related traits. Mechanistically, aberrant IL-17 signals through NF-κB in epidermal cells to impair homeostatic functions while promoting an inflammatory state. Our results indicate that aged skin shows signs of chronic inflammation and that increased IL-17 signaling could be targeted to prevent age-associated skin ailments.</span></p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37291218',
'doi' => '10.1038/s43587-023-00431-z',
'modified' => '2023-06-14 15:56:56',
'created' => '2023-06-13 21:11:31',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4141',
'name' => 'Transgenic mice for in vivo epigenome editing with CRISPR-based systems',
'authors' => 'Gemberling, M. et al.',
'description' => '<p>The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chromatin-modifying domains to the nuclease-deactivated form of Cas9 (dCas9) has enabled targeted gene activation or repression in both cultured cells and in vivo in animal models. However, delivery of the large dCas9 fusion proteins to target cell types and tissues is an obstacle to widespread adoption of these tools for in vivo studies. Here we describe the generation and validation of two conditional transgenic mouse lines for targeted gene regulation, Rosa26:LSL-dCas9-p300 for gene activation and Rosa26:LSL-dCas9-KRAB for gene repression. Using the dCas9p300 and dCas9KRAB transgenic mice we demonstrate activation or repression of genes in both the brain and liver in vivo, and T cells and fibroblasts ex vivo. We show gene regulation and targeted epigenetic modification with gRNAs targeting either transcriptional start sites (TSS) or distal enhancer elements, as well as corresponding changes to downstream phenotypes. These mouse lines are convenient and valuable tools for facile, temporally controlled, and tissue-restricted epigenome editing and manipulation of gene expression in vivo.</p>',
'date' => '2021-03-01',
'pmid' => 'https://doi.org/10.1101%2F2021.03.08.434430',
'doi' => '10.1101/2021.03.08.434430',
'modified' => '2021-12-13 09:23:10',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4144',
'name' => 'REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR',
'authors' => 'Suh, J. L. et al.',
'description' => '<p>Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their misregulation associated with human disease including cancer, CBX proteins are attractive targets for small molecule chemical probe development. Here, we have used a quantitative and target-specific cellular assay to discover a potent positive allosteric modulator (PAM) of CBX8. The PAM activity of UNC7040 antagonizes H3K27me3 binding by CBX8 while increasing interactions with nucleic acids and participation in variant PRC1. We show that treatment with UNC7040 leads to efficient PRC1 chromatin eviction, loss of silencing and reduced proliferation across different cancer cell lines. Our discovery and characterization of UNC7040 not only revealed the most cellularly potent CBX8-specific chemical probe to date, but also corroborates a mechanism of polycomb regulation by non-histone lysine methylated interaction partners.</p>',
'date' => '2021-02-01',
'pmid' => 'https://doi.org/10.1101%2F2021.02.23.432388',
'doi' => '10.1101/2021.02.23.432388',
'modified' => '2021-12-13 09:35:04',
'created' => '2021-12-06 15:53:19',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3961',
'name' => 'Estrogen induces dynamic ERα and RING1B recruitment to control gene and enhancer activities in luminal breast cancer',
'authors' => 'Zhang Yusheng, Chan Ho Lam, Garcia-Martinez Liliana, Karl Daniel L., Weich Natalia, Slingerland Joyce M., Verdun Ramiro E., Morey Lluis',
'description' => '<p>RING1B, a core Polycomb repressive complex 1 subunit, is a histone H2A ubiquitin ligase essential for development. RING1B is overexpressed in patients with luminal breast cancer (BC) and recruited to actively transcribed genes and enhancers co-occupied by the estrogen receptor (ER). Whether ER-induced transcriptional programs are mediated by RING1B is not understood. We show that prolonged estrogen administration induces transcriptional output and chromatin landscape fluctuations. RING1B loss impairs full estrogen-mediated gene expression and chromatin accessibility for key BC transcription factors. These effects were mediated, in part, by RING1B enzymatic activity and nucleosome binding functions. RING1B is recruited in a cyclic manner to ER, FOXA1, and GRHL2 cobound sites and regulates estrogen-induced enhancers and ER recruitment. Last, ChIP exo revealed multiple binding events of these factors at single-nucleotide resolution, including RING1B occupancy approximately 10 base pairs around ER bound sites. We propose RING1B as a key regulator of the dynamic, liganded-ER transcriptional regulatory circuit in luminal BC.</p>',
'date' => '2020-06-12',
'pmid' => 'https://advances.sciencemag.org/content/6/23/eaaz7249.full',
'doi' => '10.1126/sciadv.aaz7249',
'modified' => '2020-08-12 09:48:21',
'created' => '2020-08-10 12:12:25',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(
(int) 0 => array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
[maximum depth reached]
)
)
),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '3288',
'name' => 'Sonication beads SDS US en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '3286',
'name' => 'Sonication beads SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '3281',
'name' => 'Sonication beads SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '3285',
'name' => 'Sonication beads SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '3284',
'name' => 'Sonication beads SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '3283',
'name' => 'Sonication beads SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '3287',
'name' => 'Sonication beads SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-JP-ja-GHS_1_0.pdf',
'countries' => 'JP',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = '<blockquote><p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p><cite>Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford</cite></blockquote>
'
$featured_testimonials = ''
$testimonial = array(
'id' => '37',
'name' => 'Reproducible Chromatin Shearing',
'description' => '<p>Diagenode has provided a Bioruptor<sup><strong>®</strong></sup> Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.</p>',
'author' => 'Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford',
'featured' => false,
'slug' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-22 10:35:44',
'created' => '2015-10-22 09:23:04',
'ProductsTestimonial' => array(
'id' => '52',
'product_id' => '1875',
'testimonial_id' => '37'
)
)
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200016_tube_holder.jpg" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01200016</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'B01200016',
'350',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml sonication accessories for Picoruptor</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '1796',
'antibody_id' => null,
'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico',
'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '1 pack',
'catalog_number' => 'B01200016',
'old_catalog_number' => 'O-ring-15',
'sf_code' => 'B01200016-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '300',
'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
'price_CNY' => '',
'price_AUD' => '875',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack',
'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'meta_keywords' => '',
'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico',
'modified' => '2022-01-25 04:00:28',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
'id' => '1763',
'product_id' => '1875',
'related_id' => '1796'
),
'Image' => array(
(int) 0 => array(
'id' => '116',
'name' => 'product/shearing_technologies/B01200016_tube_holder.jpg',
'alt' => 'some alt',
'modified' => '2015-06-10 17:28:55',
'created' => '2015-06-10 17:28:55',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '4',
'name' => 'Chromatin shearing with the Bioruptor<sup>®</sup> Pico 500 µl - 2 ml',
'description' => '<p><span>Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. </span><span>2 ml nuclei aliquots are sonicated with the Bioruptor</span><span>® </span><span>Pico in 15 ml Bioruptor</span><span>® </span><span>Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF). </span></p>',
'image_id' => '212',
'type' => 'Protocol',
'url' => 'files/protocols/15ml_sonication_tube_protocol.pdf',
'slug' => '15ml-sonication-tube-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-01-19 13:51:19',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '44',
'product_id' => '1875',
'protocol_id' => '4'
)
)
$document = array(
'id' => '791',
'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ',
'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>',
'image_id' => null,
'type' => 'Quick guide',
'url' => 'files/organigram/bioruptor-organigram-tubes.pdf',
'slug' => 'bioruptor-organigram-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:18:51',
'created' => '2015-07-24 16:46:15',
'ProductsDocument' => array(
'id' => '736',
'product_id' => '1875',
'document_id' => '791'
)
)
$sds = array(
'id' => '3282',
'name' => 'Sonication beads SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Sonication_beads/SDS-C01020031-Sonication_beads-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2023-01-30 16:33:15',
'created' => '2023-01-30 16:33:15',
'ProductsSafetySheet' => array(
'id' => '5322',
'product_id' => '1875',
'safety_sheet_id' => '3282'
)
)
$publication = array(
'id' => '3406',
'name' => 'The effect of Jun dimerization on neurite outgrowth and motif binding.',
'authors' => 'Danzi MC, Mehta ST, Dulla K, Zunino G, Cooper DJ, Bixby JL, Lemmon VP',
'description' => '<p>Axon regeneration is a necessary step toward functional recovery after spinal cord injury. The AP-1 transcription factor c-Jun has long been known to play an important role in directing the transcriptional response of Dorsal Root Ganglion (DRG) neurons to peripheral axotomy that results in successful axon regeneration. Here we performed ChIPseq for Jun in mouse DRG neurons after a sciatic nerve crush or sham surgery in order to measure the changes in Jun's DNA binding in response to peripheral axotomy. We found that the majority of Jun's injury-responsive changes in DNA binding occur at putative enhancer elements, rather than proximal to transcription start sites. We also used a series of single polypeptide chain tandem transcription factors to test the effects of different Jun-containing dimers on neurite outgrowth in DRG, cortical and hippocampal neurons. These experiments demonstrated that dimers composed of Jun and Atf3 promoted neurite outgrowth in rat CNS neurons as well as mouse DRG neurons. Our work provides new insight into the mechanisms underlying Jun's role in axon regeneration.</p>',
'date' => '2018-08-03',
'pmid' => 'http://www.pubmed.gov/30077771',
'doi' => '10.1016/j.mcn.2018.08.001',
'modified' => '2018-11-09 11:20:24',
'created' => '2018-11-08 12:59:45',
'ProductsPublication' => array(
'id' => '2856',
'product_id' => '1875',
'publication_id' => '3406'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30077771" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
