| Bioruptor® Standard manual MANUAL Diagenode’s Bioruptor® Standard uses a gentle method of sonication to retain the ... | Download |
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| Which tubes for which Bioruptor®? QUICK GUIDE Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu... | Download |
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Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'product' => array( 'Product' => array( 'id' => '1794', 'antibody_id' => null, 'name' => '15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus', 'description' => '<p><span>The attachment for 15 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 15 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200013', 'old_catalog_number' => 'UCD-pack 15', 'sf_code' => 'B01200013-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1600', 'price_USD' => '1850', 'price_GBP' => '1400', 'price_JPY' => '250640', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2017-08-19 10:36:46', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $meta_title = '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $product = array( 'Product' => array( 'id' => '1794', 'antibody_id' => null, 'name' => '15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus', 'description' => '<p><span>The attachment for 15 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 15 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200013', 'old_catalog_number' => 'UCD-pack 15', 'sf_code' => 'B01200013-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1600', 'price_USD' => '1850', 'price_GBP' => '1400', 'price_JPY' => '250640', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2017-08-19 10:36:46', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p> <script>// <![CDATA[ document.addEventListener("DOMContentLoaded", function() { var iframes = document.getElementsByTagName("iframe"); for (var i = 0; i < iframes.length; i++) { if (iframes[i].src === "https://www.selectscience.net/review_plugin_new_widget.aspx?prodID=197278&numRv=1") { iframes[i].classList.add("hidden"); } } }); // ]]></script> </p> <div class="row"> <div class="small-12 medium-12 large-12 columns"><br /><br /> <p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020001', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22500', 'price_USD' => '29500', 'price_GBP' => '19500', 'price_JPY' => '3524625', 'price_CNY' => '/', 'price_AUD' => '73750', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2024-08-22 15:29:44', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1811', 'antibody_id' => null, 'name' => 'O-Ring for 15 ml tube holder', 'description' => '<p><span>This product has been designed for a perfect sealing of the 15 ml adaptors. Both adaptors and O rings are accessories of the Bioruptor<sup>®</sup> Standard and Plus.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '6 pc', 'catalog_number' => 'B01200203', 'old_catalog_number' => 'P-10-A', 'sf_code' => 'B01200203-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '25', 'price_USD' => '35', 'price_GBP' => '25', 'price_JPY' => '3915', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'o-ring-for-15-ml-tube-holder-6-pc', 'meta_title' => 'O-Ring for 15 ml tube holder', 'meta_keywords' => '', 'meta_description' => 'O-Ring for 15 ml tube holder', 'modified' => '2015-07-27 11:27:00', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2621', 'antibody_id' => null, 'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 pc', 'catalog_number' => 'C30010009', 'old_catalog_number' => 'M-UN-15', 'sf_code' => 'C30010009-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '100', 'price_USD' => '120', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tpx-tubes-50-pc', 'meta_title' => '15 ml TPX tubes', 'meta_keywords' => '', 'meta_description' => '15 ml TPX tubes', 'modified' => '2017-08-19 18:39:10', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '131', 'name' => 'product/shearing_technologies/B01200013_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '36', 'name' => 'Activity Test - Procedure for immunoprecipitation of native chromatin proteins', 'description' => '<p><span>Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate” team from the University Paris Descartes developed a protocol allowing the immunoprecipitation of chromatin proteins prior the analysis of their activity. They used the Bioruptor</span><span>® </span><span>in the initial step of the procedure to release this specific protein, which is advantageous in preserving protein quality. </span></p>', 'image_id' => '202', 'type' => 'Protocol', 'url' => 'files/protocols/activity_test_bioruptor_protocol.pdf', 'slug' => 'activity-test-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:47:12', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'name' => 'Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL', 'description' => '<p><span>For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respectively). To guarantee homogeneity of sonication, the tube holders should always be completely filled with tubes. </span></p>', 'image_id' => '221', 'type' => 'Protocol', 'url' => 'files/protocols/Bacterial_Cell_Disruption_with_Bioruptor_protocol.pdf', 'slug' => 'bacterial-cell-disruption-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-04-03 13:31:51', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '27', 'name' => 'Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus', 'description' => '<p>Standard procedure to shear Chromatin from Yeast. </p>', 'image_id' => '218', 'type' => 'Protocol', 'url' => 'files/protocols/Chromatin_Shearing_in_Yeast_Cells_with_Bioruptor_protocol.pdf', 'slug' => 'chromatin-shearing-in-yeast-cells-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-17 19:32:52', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '24', 'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®', 'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>', 'image_id' => '220', 'type' => 'Protocol', 'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf', 'slug' => 'western-blot-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:39:29', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. 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Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673f553b89c6d"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-1784" class="alert button expand" form="Quote-1784" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else { $("#Quote-1784 #state-1784").parent().parent().hide(); $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Plus sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/o-ring-for-15-ml-tube-holder-6-pc"><img src="/img/product/shearing_technologies/B01200203%20_ring_15ml.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200203</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1811" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1811" id="CartAdd/1811Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1811" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>O-Ring for 15 ml tube holder個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="o-ring-for-15-ml-tube-holder-6-pc" data-reveal-id="cartModal-1811" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">O-Ring for 15 ml tube holder</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010009</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml Bioruptor<sup>®</sup> Plus TPX tubes個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C20000021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2618" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2618" id="CartAdd/2618Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2618" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Protein Extraction beads個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads', 'C20000021', '100', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" 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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020001', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22500', 'price_USD' => '29500', 'price_GBP' => '19500', 'price_JPY' => '3524625', 'price_CNY' => '/', 'price_AUD' => '73750', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2024-08-22 15:29:44', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1811', 'antibody_id' => null, 'name' => 'O-Ring for 15 ml tube holder', 'description' => '<p><span>This product has been designed for a perfect sealing of the 15 ml adaptors. Both adaptors and O rings are accessories of the Bioruptor<sup>®</sup> Standard and Plus.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '6 pc', 'catalog_number' => 'B01200203', 'old_catalog_number' => 'P-10-A', 'sf_code' => 'B01200203-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '25', 'price_USD' => '35', 'price_GBP' => '25', 'price_JPY' => '3915', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'o-ring-for-15-ml-tube-holder-6-pc', 'meta_title' => 'O-Ring for 15 ml tube holder', 'meta_keywords' => '', 'meta_description' => 'O-Ring for 15 ml tube holder', 'modified' => '2015-07-27 11:27:00', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2621', 'antibody_id' => null, 'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 pc', 'catalog_number' => 'C30010009', 'old_catalog_number' => 'M-UN-15', 'sf_code' => 'C30010009-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '100', 'price_USD' => '120', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tpx-tubes-50-pc', 'meta_title' => '15 ml TPX tubes', 'meta_keywords' => '', 'meta_description' => '15 ml TPX tubes', 'modified' => '2017-08-19 18:39:10', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '131', 'name' => 'product/shearing_technologies/B01200013_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '36', 'name' => 'Activity Test - Procedure for immunoprecipitation of native chromatin proteins', 'description' => '<p><span>Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate” team from the University Paris Descartes developed a protocol allowing the immunoprecipitation of chromatin proteins prior the analysis of their activity. They used the Bioruptor</span><span>® </span><span>in the initial step of the procedure to release this specific protein, which is advantageous in preserving protein quality. </span></p>', 'image_id' => '202', 'type' => 'Protocol', 'url' => 'files/protocols/activity_test_bioruptor_protocol.pdf', 'slug' => 'activity-test-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:47:12', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'name' => 'Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL', 'description' => '<p><span>For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respectively). To guarantee homogeneity of sonication, the tube holders should always be completely filled with tubes. </span></p>', 'image_id' => '221', 'type' => 'Protocol', 'url' => 'files/protocols/Bacterial_Cell_Disruption_with_Bioruptor_protocol.pdf', 'slug' => 'bacterial-cell-disruption-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-04-03 13:31:51', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '27', 'name' => 'Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus', 'description' => '<p>Standard procedure to shear Chromatin from Yeast. </p>', 'image_id' => '218', 'type' => 'Protocol', 'url' => 'files/protocols/Chromatin_Shearing_in_Yeast_Cells_with_Bioruptor_protocol.pdf', 'slug' => 'chromatin-shearing-in-yeast-cells-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-17 19:32:52', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '24', 'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®', 'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. 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value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else { $("#Quote-1784 #state-1784").parent().parent().hide(); $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Plus sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/o-ring-for-15-ml-tube-holder-6-pc"><img src="/img/product/shearing_technologies/B01200203%20_ring_15ml.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200203</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1811" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1811" id="CartAdd/1811Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1811" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>O-Ring for 15 ml tube holder個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="o-ring-for-15-ml-tube-holder-6-pc" data-reveal-id="cartModal-1811" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">O-Ring for 15 ml tube 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value="2621" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml Bioruptor<sup>®</sup> Plus TPX tubes個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C20000021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2618" class="reveal-modal small" data-reveal 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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020001', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22500', 'price_USD' => '29500', 'price_GBP' => '19500', 'price_JPY' => '3524625', 'price_CNY' => '/', 'price_AUD' => '73750', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2024-08-22 15:29:44', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1811', 'antibody_id' => null, 'name' => 'O-Ring for 15 ml tube holder', 'description' => '<p><span>This product has been designed for a perfect sealing of the 15 ml adaptors. Both adaptors and O rings are accessories of the Bioruptor<sup>®</sup> Standard and Plus.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '6 pc', 'catalog_number' => 'B01200203', 'old_catalog_number' => 'P-10-A', 'sf_code' => 'B01200203-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '25', 'price_USD' => '35', 'price_GBP' => '25', 'price_JPY' => '3915', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'o-ring-for-15-ml-tube-holder-6-pc', 'meta_title' => 'O-Ring for 15 ml tube holder', 'meta_keywords' => '', 'meta_description' => 'O-Ring for 15 ml tube holder', 'modified' => '2015-07-27 11:27:00', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2621', 'antibody_id' => null, 'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 pc', 'catalog_number' => 'C30010009', 'old_catalog_number' => 'M-UN-15', 'sf_code' => 'C30010009-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '100', 'price_USD' => '120', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tpx-tubes-50-pc', 'meta_title' => '15 ml TPX tubes', 'meta_keywords' => '', 'meta_description' => '15 ml TPX tubes', 'modified' => '2017-08-19 18:39:10', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '131', 'name' => 'product/shearing_technologies/B01200013_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '36', 'name' => 'Activity Test - Procedure for immunoprecipitation of native chromatin proteins', 'description' => '<p><span>Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate” team from the University Paris Descartes developed a protocol allowing the immunoprecipitation of chromatin proteins prior the analysis of their activity. They used the Bioruptor</span><span>® </span><span>in the initial step of the procedure to release this specific protein, which is advantageous in preserving protein quality. </span></p>', 'image_id' => '202', 'type' => 'Protocol', 'url' => 'files/protocols/activity_test_bioruptor_protocol.pdf', 'slug' => 'activity-test-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:47:12', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'name' => 'Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL', 'description' => '<p><span>For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respectively). To guarantee homogeneity of sonication, the tube holders should always be completely filled with tubes. </span></p>', 'image_id' => '221', 'type' => 'Protocol', 'url' => 'files/protocols/Bacterial_Cell_Disruption_with_Bioruptor_protocol.pdf', 'slug' => 'bacterial-cell-disruption-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-04-03 13:31:51', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '27', 'name' => 'Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus', 'description' => '<p>Standard procedure to shear Chromatin from Yeast. </p>', 'image_id' => '218', 'type' => 'Protocol', 'url' => 'files/protocols/Chromatin_Shearing_in_Yeast_Cells_with_Bioruptor_protocol.pdf', 'slug' => 'chromatin-shearing-in-yeast-cells-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-17 19:32:52', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '24', 'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®', 'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>', 'image_id' => '220', 'type' => 'Protocol', 'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf', 'slug' => 'western-blot-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:39:29', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673f553b89c6d"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-1784" class="alert button expand" form="Quote-1784" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else { $("#Quote-1784 #state-1784").parent().parent().hide(); $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Plus sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/o-ring-for-15-ml-tube-holder-6-pc"><img src="/img/product/shearing_technologies/B01200203%20_ring_15ml.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200203</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1811" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1811" id="CartAdd/1811Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1811" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>O-Ring for 15 ml tube holder個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="o-ring-for-15-ml-tube-holder-6-pc" data-reveal-id="cartModal-1811" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">O-Ring for 15 ml tube holder</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010009</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml Bioruptor<sup>®</sup> Plus TPX tubes個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C20000021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2618" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2618" id="CartAdd/2618Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2618" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Protein Extraction beads個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads', 'C20000021', '100', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads', 'C20000021', '100', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-beads-20-g" data-reveal-id="cartModal-2618" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Protein Extraction beads</h6> </div> </div> </li> ' $related = array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. 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Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>', 'image_id' => '220', 'type' => 'Protocol', 'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf', 'slug' => 'western-blot-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:39:29', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '83', 'product_id' => '1794', 'protocol_id' => '24' ) ) $document = array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( 'id' => '2220', 'product_id' => '1794', 'document_id' => '791' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'product' => array( 'Product' => array( 'id' => '1794', 'antibody_id' => null, 'name' => '15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus', 'description' => '<p><span>The attachment for 15 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 15 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200013', 'old_catalog_number' => 'UCD-pack 15', 'sf_code' => 'B01200013-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1600', 'price_USD' => '1850', 'price_GBP' => '1400', 'price_JPY' => '250640', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2017-08-19 10:36:46', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $meta_title = '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $product = array( 'Product' => array( 'id' => '1794', 'antibody_id' => null, 'name' => '15 ml tube holder for Bioruptor<sup>®</sup> Standard & Bioruptor<sup>®</sup> Plus', 'description' => '<p><span>The attachment for 15 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 15 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200013', 'old_catalog_number' => 'UCD-pack 15', 'sf_code' => 'B01200013-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1600', 'price_USD' => '1850', 'price_GBP' => '1400', 'price_JPY' => '250640', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2017-08-19 10:36:46', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '1784', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Plus sonication device', 'description' => '<p> <script>// <![CDATA[ document.addEventListener("DOMContentLoaded", function() { var iframes = document.getElementsByTagName("iframe"); for (var i = 0; i < iframes.length; i++) { if (iframes[i].src === "https://www.selectscience.net/review_plugin_new_widget.aspx?prodID=197278&numRv=1") { iframes[i].classList.add("hidden"); } } }); // ]]></script> </p> <div class="row"> <div class="small-12 medium-12 large-12 columns"><br /><br /> <p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020001', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22500', 'price_USD' => '29500', 'price_GBP' => '19500', 'price_JPY' => '3524625', 'price_CNY' => '/', 'price_AUD' => '73750', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2024-08-22 15:29:44', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1811', 'antibody_id' => null, 'name' => 'O-Ring for 15 ml tube holder', 'description' => '<p><span>This product has been designed for a perfect sealing of the 15 ml adaptors. Both adaptors and O rings are accessories of the Bioruptor<sup>®</sup> Standard and Plus.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '6 pc', 'catalog_number' => 'B01200203', 'old_catalog_number' => 'P-10-A', 'sf_code' => 'B01200203-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '25', 'price_USD' => '35', 'price_GBP' => '25', 'price_JPY' => '3915', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'o-ring-for-15-ml-tube-holder-6-pc', 'meta_title' => 'O-Ring for 15 ml tube holder', 'meta_keywords' => '', 'meta_description' => 'O-Ring for 15 ml tube holder', 'modified' => '2015-07-27 11:27:00', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '2621', 'antibody_id' => null, 'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 pc', 'catalog_number' => 'C30010009', 'old_catalog_number' => 'M-UN-15', 'sf_code' => 'C30010009-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '100', 'price_USD' => '120', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-tpx-tubes-50-pc', 'meta_title' => '15 ml TPX tubes', 'meta_keywords' => '', 'meta_description' => '15 ml TPX tubes', 'modified' => '2017-08-19 18:39:10', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptors® used in combination with the Protein Extraction beads (diameter < 1 mm) ensure efficient disruption, homogenization and high yields of proteins from tissues.</span></p>', 'label1' => 'Characteristics', 'info1' => '<p><span>The Bioruptor® Standard, Plus or Pico used in combinaton with the Protein Extraction </span><span>beads offers unique benefits for tissue disruption and homogenization:</span></p> <ul> <li>Fast and simple</li> <li>No contamination between samples</li> <li>Efficient</li> <li>Gentle processing</li> <li>Reproducible</li> <li>Temperature controlled</li> <li>Multiplexing capability</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 g', 'catalog_number' => 'C20000021', 'old_catalog_number' => 'AL-003-0020', 'sf_code' => 'C20000021-', 'type' => 'REF', 'search_order' => '01-Accessory', 'price_EUR' => '95', 'price_USD' => '100', 'price_GBP' => '90', 'price_JPY' => '14880', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'protein-extraction-beads-20-g', 'meta_title' => 'Protein Extraction beads', 'meta_keywords' => '', 'meta_description' => 'Protein Extraction beads', 'modified' => '2020-05-27 14:42:40', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '131', 'name' => 'product/shearing_technologies/B01200013_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '36', 'name' => 'Activity Test - Procedure for immunoprecipitation of native chromatin proteins', 'description' => '<p><span>Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate” team from the University Paris Descartes developed a protocol allowing the immunoprecipitation of chromatin proteins prior the analysis of their activity. They used the Bioruptor</span><span>® </span><span>in the initial step of the procedure to release this specific protein, which is advantageous in preserving protein quality. </span></p>', 'image_id' => '202', 'type' => 'Protocol', 'url' => 'files/protocols/activity_test_bioruptor_protocol.pdf', 'slug' => 'activity-test-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:47:12', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'name' => 'Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL', 'description' => '<p><span>For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. C30010010) or 10 ml tubes (Cat. No. C30010012) and the corresponding tube holders (Cat. No. B01200011 and B01200012, respectively). To guarantee homogeneity of sonication, the tube holders should always be completely filled with tubes. </span></p>', 'image_id' => '221', 'type' => 'Protocol', 'url' => 'files/protocols/Bacterial_Cell_Disruption_with_Bioruptor_protocol.pdf', 'slug' => 'bacterial-cell-disruption-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-04-03 13:31:51', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '27', 'name' => 'Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus', 'description' => '<p>Standard procedure to shear Chromatin from Yeast. </p>', 'image_id' => '218', 'type' => 'Protocol', 'url' => 'files/protocols/Chromatin_Shearing_in_Yeast_Cells_with_Bioruptor_protocol.pdf', 'slug' => 'chromatin-shearing-in-yeast-cells-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-17 19:32:52', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '24', 'name' => 'Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®', 'description' => '<p><span>The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>', 'image_id' => '220', 'type' => 'Protocol', 'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf', 'slug' => 'western-blot-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:39:29', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. 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(WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else if (this.value == 'CA') { $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else { $("#Quote-1784 #state-1784").parent().parent().hide(); $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Plus sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/o-ring-for-15-ml-tube-holder-6-pc"><img src="/img/product/shearing_technologies/B01200203%20_ring_15ml.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200203</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1811" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1811" id="CartAdd/1811Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1811" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>O-Ring for 15 ml tube holder個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('O-Ring for 15 ml tube holder', 'B01200203', '35', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="o-ring-for-15-ml-tube-holder-6-pc" data-reveal-id="cartModal-1811" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">O-Ring for 15 ml tube 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value="2621" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml Bioruptor<sup>®</sup> Plus TPX tubes個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes', 'C30010009', '120', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/protein-extraction-beads-20-g"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C20000021</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2618" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2618" id="CartAdd/2618Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2618" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Protein Extraction beads個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads', 'C20000021', '100', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction beads', 'C20000021', '100', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-beads-20-g" data-reveal-id="cartModal-2618" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Protein Extraction beads</h6> </div> </div> </li> ' $related = array( 'id' => '2618', 'antibody_id' => null, 'name' => 'Protein Extraction beads', 'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. 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Using <a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device">Bioruptor</a></span><a href="https://www.diagenode.com/categories/bioruptor-shearing-device" title="Bioruptor Shearing device"><span>®</span></a><span>, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins. </span></p>', 'image_id' => '220', 'type' => 'Protocol', 'url' => 'files/protocols/western-blot-with-bioruptor-protocol.pdf', 'slug' => 'western-blot-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:39:29', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '83', 'product_id' => '1794', 'protocol_id' => '24' ) ) $document = array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( 'id' => '2220', 'product_id' => '1794', 'document_id' => '791' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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