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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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'name' => 'Ago (Argonautes) Antibody ',
'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–859 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<td>IP (RIP)</td>
<td>8 μg per IP</td>
<td>Fig 1</td>
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<td>HITS-CLIP</td>
<td>-</td>
<td>Fig 2</td>
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<td><span>Fig 3</span></td>
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<td>Fig 4</td>
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'name' => 'Ago (Argonautes) Antibody ',
'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–859 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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'label1' => 'Validation Data',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
</div>
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Argonautes are needed for miRNA-induced silencing and contain two conserved RNA binding domains: a PAZ domain that can bind the single stranded 3’ end of the mature miRNA and a PIWI domain that structurally resembles ribonuclease-H and functions to interact with the 5’ end of the guide strand.
Some argonautes, for example human Ago2, cleave target transcripts directly; argonautes may also recruit additional proteins to achieve translational repression. The human genome encodes eight Argonaute proteins divided by sequence similarities into two families: AGO (with four members present in all mammalian cells and called E1F2C/hAgo in humans), and PIWI (found in the germ line and hematopoietic stem cells).',
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<td>IP (RIP)</td>
<td>8 μg per IP</td>
<td>Fig 1</td>
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<tr>
<td>HITS-CLIP</td>
<td>-</td>
<td>Fig 2</td>
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<td>WB</td>
<td>1:500</td>
<td><span>Fig 3</span></td>
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<td>IF</td>
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<td>Fig 4</td>
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<td>IHC</td>
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'name' => 'Ago (Argonautes) Antibody ',
'description' => '<p>Purified monoclonal antibody prepared by immunizing mice with recombinant human Ago2 protein (GST fusion protein containing amino acids 47–859 of Ago2). The antibody also recognizes other Ago family members although with the highest affinity for Ago2.</p>',
'label1' => 'Validation Data',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig1.png" alt="Ago (Argonautes) Antibody validated in IP" caption="false" width="278" height="164" /></p>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_fig2.png" alt="coimmunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200167-wb.png" alt="Ago (Argonautes) Antibody validated in WB" caption="false" width="278" height="359" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200167_if.jpg" alt="Ago (Argonautes) Antibody validated in IF" caption="false" width="278" height="91" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<p><small><strong> Figure 1. Immunoprecipitation of endogenous Argonaute proteins using the Diagenode Ago monoclonal antibody </strong><br />Hela cells (10 cm dish per IP) were subjected to UV crosslinking, lysed in 70 μl of 1xPXL buffer (1xPBS, 0.1% SDS, 0.5% Nadeoxycholate, 0.5% NP-40) by incubating on ice for 10 min, then treated with DNase I. Dynabeads Protein A (40 μl each) were first incubated with 12 μg anti-mouse IgG, used as a bridge antibody, briefly washed, and then incubated with Diagenode Ago monoclonal antibody (Cat. No. C15200167) (4 or 12 μg), or non-immune mouse IgG control (4 or 12 μg). The cell lysate and Dynabeads were mixed and incubated at 4°C for overnight. The beads were washed twice each at 4°C for 5 min using 500 μl of (i) 1 x PXL buffer, (ii) 5 x PXL (5xPBS, 0.1% SDS, 0.5% Na-deoxycholate, 0.5% NP-40), and (iii) 1 x PNK (50 mM Tris pH7.4, 10 mM MgCl2, 0.5% NP-40). Ten μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded on SDS-PAGE gel and subjected to western blot analysis using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) and the ECL plus reagent.</small></p>
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<p><small><strong> Figure 2. Allelic imbalance Ago-RIP assay with the Diagenode Argonautes monoclonal antibody </strong><br />The goal of this experiment is to show the preferential coimmunoprecipitation of the miRNA targeted alleles using Diagenode Ago monoclonal antibody (Cat No. C15200167). Briefly, Hela cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele) Cell lysate was immunoprecipitated with Protein G agarose beads incubated with Diagenode Ago monoclonal antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. “The predicted miRNA target alleles were efficiently coimmunoprecipitated by the Diagenode Ago monoclonal antibody (Cat. No. C15200167). More details can be found in Takeda H, Charlier C, Farnir F, Georges M., RNA, 2010,1854-63, Epub 2010, Aug 2. Results were kindly provided by Haruko Takeda (Unit of Animal Genomics, GIGA Research Center, Faculty of Veterinary Medicine, University of Liège, 4000-Liège, Belgium).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode Ago monoclonal antibody</strong><br />Whole cell extracts (40 µg) from HeLa cells were analysed by Western blot using the Diagenode Ago monoclonal antibody (Cat. No. C15200167) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunofluorescence using the Diagenode Ago monoclonal antibody</strong><br />HeLa cells were stained with the Diagenode antibody against Ago (cat. No. C15200167) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the Ago antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.</small></p>
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)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
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'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
'id' => '1951',
'product_id' => '1995',
'application_id' => '19'
)
)
$slugs = array(
(int) 0 => 'western-blot-antibodies'
)
$applications = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'jpn'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '2017',
'product_id' => '1995',
'document_id' => '38'
)
)
$sds = array(
'id' => '554',
'name' => 'Ago Argonautes antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Ago/SDS-C15200167-Ago_Argonautes_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 13:50:11',
'created' => '2020-07-01 13:50:11',
'ProductsSafetySheet' => array(
'id' => '1060',
'product_id' => '1995',
'safety_sheet_id' => '554'
)
)
$publication = array(
'id' => '1848',
'name' => 'Differential RISC association of endogenous human microRNAs predicts their inhibitory potential.',
'authors' => 'Flores O, Kennedy EM, Skalsky RL, Cullen BR',
'description' => 'It has previously been assumed that the generally high stability of microRNAs (miRNAs) reflects their tight association with Argonaute (Ago) proteins, essential components of the RNA-induced silencing complex (RISC). However, recent data have suggested that the majority of mature miRNAs are not, in fact, Ago associated. Here, we demonstrate that endogenous human miRNAs vary widely, by >100-fold, in their level of RISC association and show that the level of Ago binding is a better indicator of inhibitory potential than is the total level of miRNA expression. While miRNAs of closely similar sequence showed comparable levels of RISC association in the same cell line, these varied between different cell types. Moreover, the level of RISC association could be modulated by overexpression of complementary target mRNAs. Together, these data indicate that the level of RISC association of a given endogenous miRNA is regulated by the available RNA targetome and predicts miRNA function.',
'date' => '2014-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/24464996',
'doi' => '',
'modified' => '2015-07-24 15:39:01',
'created' => '2015-07-24 15:39:01',
'ProductsPublication' => array(
'id' => '759',
'product_id' => '1995',
'publication_id' => '1848'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/24464996" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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