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Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 - 1:1000 | Fig 1 |
| IHC | 1:100 - 500 | |
| Immunofluorescence | 1:100 - 500 |
Figure 1. ELISA results
ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).
| ELISA Enzyme-linked immunosorbent assay. Read more |
| IHC Immunohistochemistry Read more |
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| Datasheet ASH1L C15410304 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: ASH1L polyclonal antibody (Diagenode Cat# C15410304 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2423', 'antibody_id' => '331', 'name' => 'ASH1L polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410304-elisa.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2423', 'antibody_id' => '331', 'name' => 'ASH1L polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410304-elisa.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 11:22:53', 'created' => '2015-07-10 10:35:11', 'select_label' => '331 - ASH1L polyclonal antibody - Classic (001 - 1 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'ASH1L polyclonal antibody - Classic' $meta_title = 'ASH1L polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2423', 'antibody_id' => '331', 'name' => 'ASH1L polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410304-elisa.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 11:22:53', 'created' => '2015-07-10 10:35:11', 'select_label' => '331 - ASH1L polyclonal antibody - Classic (001 - 1 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '413', 'name' => 'Datasheet ASH1L C15410304', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ASH1L_C15410304.pdf', 'slug' => 'datasheet-ash1l-c15410304', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( 'id' => '5056', 'product_id' => '2423', 'application_id' => '29' ) ) $slugs = array( (int) 0 => 'immunofluorescence' ) $applications = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'locale' => 'jpn' ) $description = '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>' $name = 'IF' $document = array( 'id' => '413', 'name' => 'Datasheet ASH1L C15410304', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_ASH1L_C15410304.pdf', 'slug' => 'datasheet-ash1l-c15410304', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '157', 'product_id' => '2423', 'document_id' => '413' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2423', 'antibody_id' => '331', 'name' => 'ASH1L polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410304-elisa.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 11:22:53', 'created' => '2015-07-10 10:35:11', 'select_label' => '331 - ASH1L polyclonal antibody - Classic (001 - 1 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'ASH1L polyclonal antibody - Classic' $meta_title = 'ASH1L polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2423', 'antibody_id' => '331', 'name' => 'ASH1L polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410304-elisa.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2423', 'antibody_id' => '331', 'name' => 'ASH1L polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410304-elisa.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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ELISA results</strong><br />ELISA analysis with 2 µg/100 µl antigen and serially diluted ASH1L antibody (1:500, 1:1,000, 1:2,000, 1:4,000, 1:8,000, 1:16,000, 1:32,000).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15410304', 'old_catalog_number' => '', 'sf_code' => 'C15410304-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'ash1l-polyclonal-antibody-classic-50-mg', 'meta_title' => 'ASH1L polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'ASH1L polyclonal antibody - Classic', 'modified' => '2022-01-05 15:18:04', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '331', 'name' => 'ASH1L polyclonal antibody - Classic', 'description' => 'Polyclonal antibody raised in rabbit against ASH1L, using a KLH-conjugated synthetic peptide.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1 μg/μl ', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>IHC</td> <td>1:100 - 500</td> <td></td> </tr> <tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 500</td> <td></td> </tr> </tbody> </table> <small><sup>*</sup> Optimal dilution should be determined by the end-user.</small>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '21', 'position' => '20', 'parent_id' => '4', 'name' => 'Histone modifying enzymes', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. 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