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Polyclonal antibody raised in rabbit against human BCL7C (B-cell CLL/lymphoma 7C), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.
| Lot | A384-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 – 1:1,000 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. No. CS-124-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The antibody detects both known isoforms of the protein (expected sizes: 23.4 and 26.4 kDa).
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet BCL7C CS-124-100 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: BCL7C polyclonal antibody (Diagenode Cat# C15310124 Lot# A384-004). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'BCL7C polyclonal antibody - Pioneer', 'meta_title' => 'BCL7C polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2114', 'antibody_id' => '246', 'name' => 'BCL7C polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against human BCL7C (B-cell CLL/lymphoma 7C), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig1.jpg" alt="ELISA" width="277" height="220" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig2.jpg" alt="Western blot" width="109" height="133" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. No. CS-124-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The antibody detects both known isoforms of the protein (expected sizes: 23.4 and 26.4 kDa).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310124', 'old_catalog_number' => 'CS-124-100', 'sf_code' => 'C15310124-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bcl7c-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'BCL7C polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'BCL7C polyclonal antibody - Pioneer', 'modified' => '2022-01-05 15:18:46', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '246', 'name' => 'BCL7C polyclonal antibody', 'description' => 'BCL7C (UniProt/Swiss-Prot entry Q8WUZ0) is homologous to the N-terminal region of BCL7A which was shown to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. 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Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig2.jpg" alt="Western blot" width="109" height="133" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'BCL7C polyclonal antibody - Pioneer', 'meta_title' => 'BCL7C polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2114', 'antibody_id' => '246', 'name' => 'BCL7C polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against human BCL7C (B-cell CLL/lymphoma 7C), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig1.jpg" alt="ELISA" width="277" height="220" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig2.jpg" alt="Western blot" width="109" height="133" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. No. CS-124-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'BCL7C polyclonal antibody - Pioneer', 'meta_title' => 'BCL7C polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2114', 'antibody_id' => '246', 'name' => 'BCL7C polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against human BCL7C (B-cell CLL/lymphoma 7C), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig1.jpg" alt="ELISA" width="277" height="220" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. 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Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig2.jpg" alt="Western blot" width="109" height="133" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'BCL7C polyclonal antibody - Pioneer', 'meta_title' => 'BCL7C polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2114', 'antibody_id' => '246', 'name' => 'BCL7C polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against human BCL7C (B-cell CLL/lymphoma 7C), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig1.jpg" alt="ELISA" width="277" height="220" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig2.jpg" alt="Western blot" width="109" height="133" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. No. CS-124-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. 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Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human BCL7C (Cat. No. CS-124-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:209,000. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310124_fig2.jpg" alt="Western blot" width="109" height="133" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against BCL7C</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against BCL7C (Cat. No. CS-124-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '33', 'position' => '50', 'parent_id' => '4', 'name' => 'Other antibodies', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'other-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-04 16:02:57', 'created' => '2014-12-25 12:45:50', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '755', 'name' => 'Datasheet BCL7C CS-124-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_BCL7C_CS-124-100.pdf', 'slug' => 'datasheet-bcl7c-cs-124-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(CS-124-100)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'jpn' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>' $name = 'WB' $document = array( 'id' => '755', 'name' => 'Datasheet BCL7C CS-124-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_BCL7C_CS-124-100.pdf', 'slug' => 'datasheet-bcl7c-cs-124-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '187', 'product_id' => '2114', 'document_id' => '755' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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