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BMI1 polyclonal antibody

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Catalog Number

Format

Price

C15310241
(CS-PA021-100)

100 µl

$260.00
Bulk order

Alternative names: PCGF4, RNF51, FLVI2

Polyclonal antibody raised in rabbit against mouse BMI1 (BMI1 polycomb ring finger oncogene) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.

Lot	A1026-001
Concentration	not determined
Species reactivity	Mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:300	Fig 1

^* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.

Validation Data

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse BMI1 (cat. No. CS-PA021-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,100.
実験手法
ELISA
Enzyme-linked immunosorbent assay. Read more

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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse BMI1 (cat. No. CS-PA021-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,100.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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			'description' => '<p>Alternative names: <strong>PCGF4</strong>, <strong>RNF51</strong>, <strong>FLVI2</strong></p>
<p>Polyclonal antibody raised in rabbit against mouse BMI1 (BMI1 polycomb ring finger oncogene) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.</p>',
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse BMI1 (cat. No. CS-PA021-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,100.</small></p>
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			'classification' => 'Pioneer',
			'application_table' => '<table>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>ELISA</td>
<td>1:300</td>
<td>Fig 1</td>
</tr>
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<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
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		'name' => 'BMI1 polyclonal antibody ',
		'description' => '<p>Alternative names: <strong>PCGF4</strong>, <strong>RNF51</strong>, <strong>FLVI2</strong></p>
<p>Polyclonal antibody raised in rabbit against mouse BMI1 (BMI1 polycomb ring finger oncogene) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.</p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310241-Fig1-ELISA.jpg" alt="ELISA" height="339" width="400" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse BMI1 (cat. No. CS-PA021-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,100.</small></p>
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		'description' => 'BMI1 (UniProtKB/Swiss-Prot entry P35226) is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of lysine 119 on histone H2A, rendering chromatin heritably changed in its expressibility. BMI1 is required to stimulate the E3 ubiquitin- protein ligase activity of RNF2/RING2 in the PRC1 complex.',
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<th>Suggested dilution</th>
<th>References</th>
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<td>ELISA</td>
<td>1:300</td>
<td>Fig 1</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.</small></p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet BMI1 CS-PA021-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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