Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
)
$language = 'jp'
$meta_keywords = 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode'
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '195',
'name' => 'C28020001',
'product_id' => '2855',
'modified' => '2016-11-22 15:59:19',
'created' => '2016-11-22 15:59:19'
),
'Master' => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '97',
'position' => '9',
'parent_id' => '5',
'name' => 'Exosomes',
'description' => '<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
<p style="text-align: justify;"></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
<p><span style="font-weight: 400;">Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). </span></p>
<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
<ul>
<li><span style="font-weight: 400;"><strong>Pure exosome capture</strong> - zero contamination from vesicles or aggregates</span></li>
<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
</ul>
<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
<area shape="rect" coords="920,1025,1140,1090" href="../p/captev-serum-plasma-10ml" alt="CaptEV serum plasma" />
<area shape="rect" coords="875,1100,1175,1165" href="../p/evcleaner-1-pc" alt="EVCleaner" />
<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
</map></p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'exosomes',
'cookies_tag_id' => null,
'meta_keywords' => 'Exosome,ExoIP kits,microRNA, miRNA, RNA-seq ,Diagenode',
'meta_description' => 'Diagenode offers the best tools to Isolate and enrich exosomes which can be used in broad applications such as RNA and microRNA (miRNA) analysis, Protein Analysis, Western Blot, ELISA, flow Cytometry.',
'meta_title' => 'High yields of miRNAs with our highly specific Exosome Isolation Solutions | Diagenode',
'modified' => '2024-11-07 14:37:09',
'created' => '2015-10-12 10:34:37',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$meta_canonical = 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
(int) 1 => array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
)
$pro = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/evcleaner-1-pc"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C28020001-1</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">EVCleaner</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
'id' => '4200',
'product_id' => '2854',
'related_id' => '2860'
),
'Image' => array()
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
'id' => '2291',
'product_id' => '2854',
'document_id' => '938'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
)
$language = 'jp'
$meta_keywords = 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode'
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '195',
'name' => 'C28020001',
'product_id' => '2855',
'modified' => '2016-11-22 15:59:19',
'created' => '2016-11-22 15:59:19'
),
'Master' => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '97',
'position' => '9',
'parent_id' => '5',
'name' => 'Exosomes',
'description' => '<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
<p style="text-align: justify;"></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
<p><span style="font-weight: 400;">Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). </span></p>
<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
<ul>
<li><span style="font-weight: 400;"><strong>Pure exosome capture</strong> - zero contamination from vesicles or aggregates</span></li>
<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
</ul>
<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
<area shape="rect" coords="920,1025,1140,1090" href="../p/captev-serum-plasma-10ml" alt="CaptEV serum plasma" />
<area shape="rect" coords="875,1100,1175,1165" href="../p/evcleaner-1-pc" alt="EVCleaner" />
<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
</map></p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'exosomes',
'cookies_tag_id' => null,
'meta_keywords' => 'Exosome,ExoIP kits,microRNA, miRNA, RNA-seq ,Diagenode',
'meta_description' => 'Diagenode offers the best tools to Isolate and enrich exosomes which can be used in broad applications such as RNA and microRNA (miRNA) analysis, Protein Analysis, Western Blot, ELISA, flow Cytometry.',
'meta_title' => 'High yields of miRNAs with our highly specific Exosome Isolation Solutions | Diagenode',
'modified' => '2024-11-07 14:37:09',
'created' => '2015-10-12 10:34:37',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$meta_canonical = 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
(int) 1 => array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
)
$pro = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/evcleaner-1-pc"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C28020001-1</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">EVCleaner</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
'id' => '4200',
'product_id' => '2854',
'related_id' => '2860'
),
'Image' => array()
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
'id' => '2291',
'product_id' => '2854',
'document_id' => '938'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
)
$language = 'jp'
$meta_keywords = 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode'
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '195',
'name' => 'C28020001',
'product_id' => '2855',
'modified' => '2016-11-22 15:59:19',
'created' => '2016-11-22 15:59:19'
),
'Master' => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '97',
'position' => '9',
'parent_id' => '5',
'name' => 'Exosomes',
'description' => '<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
<p style="text-align: justify;"></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
<p><span style="font-weight: 400;">Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). </span></p>
<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
<ul>
<li><span style="font-weight: 400;"><strong>Pure exosome capture</strong> - zero contamination from vesicles or aggregates</span></li>
<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
</ul>
<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
<area shape="rect" coords="920,1025,1140,1090" href="../p/captev-serum-plasma-10ml" alt="CaptEV serum plasma" />
<area shape="rect" coords="875,1100,1175,1165" href="../p/evcleaner-1-pc" alt="EVCleaner" />
<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
</map></p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'exosomes',
'cookies_tag_id' => null,
'meta_keywords' => 'Exosome,ExoIP kits,microRNA, miRNA, RNA-seq ,Diagenode',
'meta_description' => 'Diagenode offers the best tools to Isolate and enrich exosomes which can be used in broad applications such as RNA and microRNA (miRNA) analysis, Protein Analysis, Western Blot, ELISA, flow Cytometry.',
'meta_title' => 'High yields of miRNAs with our highly specific Exosome Isolation Solutions | Diagenode',
'modified' => '2024-11-07 14:37:09',
'created' => '2015-10-12 10:34:37',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$meta_canonical = 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
(int) 1 => array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
)
$pro = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/evcleaner-1-pc"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C28020001-1</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">EVCleaner</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
'id' => '4200',
'product_id' => '2854',
'related_id' => '2860'
),
'Image' => array()
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
'id' => '2291',
'product_id' => '2854',
'document_id' => '938'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'meta_title' => '',
'product' => array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
)
$language = 'jp'
$meta_keywords = 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode'
$meta_description = ''
$meta_title = ''
$product = array(
'Product' => array(
'id' => '2854',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</p>
<p></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to precipitate all extracellular vesicles(EVs) within a single sample to avoid bias. After precipitation, the exosome-rich suspension can be further purified by immunocapture with Diagenode’s ExoIP™ immunocapture kits.</p>
<h6>Advantages of CaptEV :</h6>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>Validation by electron microscopy</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
</div>
</div>
<ul>
<li>Validation by Flow Cytometry</li>
</ul>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/categories/exosome/exosome-validation-by-electron-microscopy.png" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '5 ml',
'catalog_number' => 'C28030001-5',
'old_catalog_number' => '',
'sf_code' => 'C28030001-352',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '135',
'price_USD' => '135',
'price_GBP' => '120',
'price_JPY' => '21150',
'price_CNY' => '',
'price_AUD' => '338',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-5ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:39',
'created' => '2016-11-22 15:56:43',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '195',
'name' => 'C28020001',
'product_id' => '2855',
'modified' => '2016-11-22 15:59:19',
'created' => '2016-11-22 15:59:19'
),
'Master' => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '97',
'position' => '9',
'parent_id' => '5',
'name' => 'Exosomes',
'description' => '<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;"><strong><img src="https://www.diagenode.com/img/categories/library-prep/flux-exosomes.png" alt="" usemap="#fluxmap" width="811" height="334" /></strong></h2>
<p style="text-align: justify;"></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;"></span></p>
<p><span style="font-weight: 400;">The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that <strong>exosomes</strong>, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including <strong>small non-coding RNAs</strong> (microRNAs and others) and proteins involved in intercellular communication.</span></p>
<p><span style="font-weight: 400;">Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). </span></p>
<p><span style="font-weight: 400;"></span></p>
<h3 class="red"><strong>Benefits of our optimal exosome capture solutions </strong></h3>
<ul>
<li><span style="font-weight: 400;"><strong>Pure exosome capture</strong> - zero contamination from vesicles or aggregates</span></li>
<li><span style="font-weight: 400;">Fully compatible with our <strong>D-Plex</strong> technology (<a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">D-Plex Small RNA-seq kit</a>)</span></li>
<li><span style="font-weight: 400;"><strong>High yields</strong> from exosome pre-enrichment </span></li>
<li><strong>Global</strong> and <strong>specific</strong> exosome isolation options</li>
<li><strong>Guaranteed results</strong> with high level of validation</li>
<li><strong>User-friendlly</strong> method - no ultracentrifugation needed</li>
<li>Broad applications - RNA and microRNA (miRNA) NGS analysis, protein analysis, Western blot, ELISA, flow cytometry</li>
</ul>
<h3></h3>
<div class="row" id="workflow"> <img src="https://www.diagenode.com/img/product/kits/exosome-workflow-all.png" alt="" usemap="#wfmap" width="500" height="871" /></div>
<h3 class="red"><strong>microRNAs highlight</strong><strong>ed after exosomes isolation</strong></h3>
<p>MicroRNA differential expression analysis was performed on RNA-seq data from RNA isolated from exosomes captured from HEK293T cell culture supernatant and total RNA isolated from HEK293T cells.</p>
<p>The RNA libraries were prepared using Diagenode's <a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24">RNA-seq library preparation</a> protocol for small RNA analysis. Reference genomes were obtained from the UCSC genome browser.</p>
<p><img src="https://www.diagenode.com/img/categories/exosome/micro-rnas-highlighted.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p>We observed several upregulated and downregulated microRNAs between exosomes isolated from cell culture supernatant and total HEK293T cells.</p>
<p style="padding-left: 30px;">- microRNA upregulation including miR7704, mirR4532, miR6087, miR4497, miR3687, miR19a-3p</p>
<p style="padding-left: 30px;">- microRNA downregulation including miR663a, miR4454, miR222-3p, miR196b-5p, miR10a-5p</p>
<p><map name="fluxmap">
<area shape="rect" coords="1610,865,1985,985" href="../p/CATS-Small-RNA-seq-Kit" alt="CATS Small RNA seq-Kit" />
<area shape="rect" coords="920,1025,1140,1090" href="../p/captev-serum-plasma-10ml" alt="CaptEV serum plasma" />
<area shape="rect" coords="875,1100,1175,1165" href="../p/evcleaner-1-pc" alt="EVCleaner" />
<area shape="rect" coords="940,1180,1110,1245" href="../p/exoip-composite-kit" alt="ExoIP Composite Kit" />
</map></p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'exosomes',
'cookies_tag_id' => null,
'meta_keywords' => 'Exosome,ExoIP kits,microRNA, miRNA, RNA-seq ,Diagenode',
'meta_description' => 'Diagenode offers the best tools to Isolate and enrich exosomes which can be used in broad applications such as RNA and microRNA (miRNA) analysis, Protein Analysis, Western Blot, ELISA, flow Cytometry.',
'meta_title' => 'High yields of miRNAs with our highly specific Exosome Isolation Solutions | Diagenode',
'modified' => '2024-11-07 14:37:09',
'created' => '2015-10-12 10:34:37',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$meta_canonical = 'https://www.diagenode.com/jp/p/captev-cell-culture-10ml'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array(
(int) 0 => array(
'id' => '2855',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '<p><span>CaptEV is a pre-enrichement reagent that concentrates all extracellular vesicules of cell culture supernatant to ensure optimal isolation and purification of exosomes.</span></p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>The CaptEV reagent has been designed to concentrate and precipitate all extracellular vesicles(EVs) within serum or plasma blood samples to avoid bias. After precipitation, the exosome-rich suspension can be further purified by:</p>
<ul>
<li>immunocapture with Diagenode’s ExoIP™ immunocapture kits for a <strong>selective</strong> isolation</li>
<li>EVCleaner Diagenode Column for a <strong>global</strong> isolation</li>
</ul>
<h6></h6>
<p></p>
<h6>Advantages of CaptEV :</h6>
<p></p>
<ul>
<li>Full recovery of EVs</li>
<li>Enhanced immunocapture</li>
<li>Better signal coverage in FACS than with other reagents</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Intact and preserved exosomes (not sticky)</li>
</ul>
<h4></h4>
<p></p>
<h4>Validation Data</h4>
<p></p>
<p>We used several methods to charachterize and validate exosome isolated with both CaptEV and <span>ExoIP™ immunocapture</span> kit:</p>
<ul>
<li>
<h6>Validation by electron microscopy </h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-2.jpg" caption="false" width="276" height="183" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>TEM picture confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples. ExoIP anti-CD81 kit was used with elution of beads and Diagenode CaptEV reagent as a pre-enrichment tool. <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<ul>
<li>
<h6>Validation by Flow Cytometry</h6>
</li>
</ul>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-3.png" width="756" height="416" caption="false" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><small>Median signal intensity from the flow cytometry analysis of the exosome capture efficiency corresponding to different pre-enrichment ways prior immunocapture (ExoIP™). <br /><br /></small></p>
<p></p>
<p></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '10 ml',
'catalog_number' => 'C28030001-10',
'old_catalog_number' => '',
'sf_code' => 'C28030001-44340',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '265',
'price_USD' => '265',
'price_GBP' => '230',
'price_JPY' => '41510',
'price_CNY' => '',
'price_AUD' => '662',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-10ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:20',
'created' => '2016-11-22 15:57:46'
),
(int) 1 => array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
)
$pro = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/jp/p/evcleaner-1-pc"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C28020001-1</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">EVCleaner</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '2860',
'antibody_id' => null,
'name' => 'EVCleaner - DISCONTINUED',
'description' => '<p>EVCleaner size exclusion columns ensure efficent separation of extracellular vesicules from unwanted size components in sample.</p>',
'label1' => 'Exosomes',
'info1' => '<p><span>Exosomes are small <strong>extracellular vesicules</strong> of 30-100 nm constitutively released under certain stimuli in biological fluids including blood, urine, saliva, breast milk, culture medium of cell cultures, etc.</span></p>
<p><span>Exosomes contain various molecular constituents of their cell of origin, including <strong>proteins</strong> and <strong>microRNA</strong>. Circulant exosomes can potentially act as cargo messenger and transfer molecules from one cell to another via membrane vesicle trafficking.</span></p>
<p><span>Exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis and functional use.</span></p>',
'label2' => 'Product description',
'info2' => '<h4>Description</h4>
<p></p>
<p>EVCleaner has been designed to collect enriched extracellular vesicles(EVs) from samples. </p>
<h6></h6>
<h6>Advantages of EVCleaner :</h6>
<p></p>
<ul>
<li>Fast, within 30 minutes</li>
<li>Ease of use</li>
<li>Reproducibility</li>
<li>Suitable for a wide range of sample </li>
<li>Suitable for routine EVs isolation</li>
<li>Intact and global exosome isolation</li>
</ul>
<h4></h4>
<p></p>
<h4>Performance Data</h4>
<p></p>
<p>The EVCleaner size exclusion column has been especially designed to enrich samples in extracellular vesicles. Thanks to a careful optimization, over 95% of soluble proteins in the sample can be discarded through the elution while a gentle recovery of all the exosomes is obtained.</p>
<p></p>
<div class="row">
<div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/product/kits/C28030001-exosome-1.png" caption="false" width="276" height="203" /></div>
<div class="small-8 medium-8 large-8 columns">
<p><span size="1" style="font-size: xx-small;">Representation of elution model. Elution changment may occur for different experimental models. Performed on HeLa cell culture meidium. </span></p>
<p><small><br /><br /></small></p>
<p></p>
</div>
</div>',
'label3' => '',
'info3' => '',
'format' => '1 pc',
'catalog_number' => 'C28020001-1',
'old_catalog_number' => '',
'sf_code' => 'C28020001-82180',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '75',
'price_USD' => '75',
'price_GBP' => '70',
'price_JPY' => '13900',
'price_CNY' => '',
'price_AUD' => '190',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => true,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'evcleaner-1-pc',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2023-03-15 09:32:14',
'created' => '2016-11-22 16:03:34',
'ProductsRelated' => array(
'id' => '4200',
'product_id' => '2854',
'related_id' => '2860'
),
'Image' => array()
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2856',
'antibody_id' => null,
'name' => 'CaptEV cell culture',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 ml',
'catalog_number' => 'C28030001-50',
'old_catalog_number' => '',
'sf_code' => 'C28030001-25190',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1010',
'price_USD' => '1010',
'price_GBP' => '865',
'price_JPY' => '158215',
'price_CNY' => '',
'price_AUD' => '2525',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '0000-00-00',
'slug' => 'captev-cell-culture-50ml',
'meta_title' => '',
'meta_keywords' => 'Exosome, Exosome enrichment, Exosome purification, microRNA, Diagenode',
'meta_description' => '',
'modified' => '2018-01-26 10:58:55',
'created' => '2016-11-22 15:58:46',
'ProductsGroup' => array(
'id' => '212',
'product_id' => '2856',
'group_id' => '195'
)
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '938',
'name' => 'CaptEV reagent',
'description' => '<p>CaptEV: EV pre-enrichment reagent</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Exosome-CaptEV-manual.pdf',
'slug' => 'exosome-captev-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-12-23 17:22:40',
'created' => '2016-12-23 17:22:40',
'ProductsDocument' => array(
'id' => '2291',
'product_id' => '2854',
'document_id' => '938'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
