ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A1131-001 |
---|---|
Concentration | not determined |
Species reactivity | Human |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:100 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CBX3 (cat. No. CS-PA014-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,250.
Datasheet CBX3 CS-PA014-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: CBX3 polyclonal antibody (Diagenode Cat# C15310234 Lot# A1131-001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CBX3 (cat. No. CS-PA014-100). The plates were coated with the peptides used for immunization of the rabbit. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'CBX3 polyclonal antibody - Pioneer', 'meta_title' => 'CBX3 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2147', 'antibody_id' => '255', 'name' => 'CBX3 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HP1gamma</strong>, <strong>HECH</strong></p> <p>Polyclonal antibody raised in rabbit against human CBX3 (chromobox homolog 3) using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310234-Fig1-ELISA.jpg" alt="ELISA" height="320" width="385" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'CBX3 polyclonal antibody - Pioneer', 'meta_title' => 'CBX3 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2147', 'antibody_id' => '255', 'name' => 'CBX3 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>HP1gamma</strong>, <strong>HECH</strong></p> <p>Polyclonal antibody raised in rabbit against human CBX3 (chromobox homolog 3) using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310234-Fig1-ELISA.jpg" alt="ELISA" height="320" width="385" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. 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