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CHD1 Antibody - ChIP-seq Grade (sample size)

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Negative IgG control

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Catalog Number

Format

Price

C15410334-20

20 µl

$120.00
Bulk order

Other format

C15410334

100 μl

Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

Lot	A301-218A1
Concentration	0.2 μg/μl
Species reactivity	Human
Type	Polyclonal ChIP grade, ChIP-seq grade
Purity	Affinity purified
Host	Rabbit
Storage Conditions	Store at 4°C.
Storage Buffer	TBS containing 0.1% BSA and 0.09% sodium azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq^*	1 μg/ChIP	Fig 1, 2
Western Blotting	1:2,000	Fig 3
IP	3 μg per IP	Fig 4

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

¹Manufactured by Bethyl Laboratories, Inc., Texas, USA

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1
ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

A.
B.
C.
D.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1
ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).

Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1
Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200.
Target Description
CHD1 (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.

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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<td>ChIP/ChIP-seq<sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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		'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>',
		'label1' => 'Validation data',
		'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>',
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		'format' => '20 µl',
		'catalog_number' => 'C15410334-20',
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		'sf_code' => 'C15410334-361',
		'type' => 'REF',
		'search_order' => '03-Antibody',
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		'price_USD' => '120',
		'price_GBP' => '105',
		'price_JPY' => '17230',
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		'price_AUD' => '300',
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		'modified' => '2021-12-23 12:19:02',
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		'host' => '*****',
		'id' => '634',
		'name' => 'CHD1 Antibody - ChIP-seq Grade (sample size)',
		'description' => 'CHD1 (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A301-218A1',
		'concentration' => '0.2 μg/μl',
		'reactivity' => 'Human',
		'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Affinity purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq<sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td><span>Western Blotting</span></td>
<td>1:2,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td><span>3 &mu;g per IP</span></td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
		'storage_conditions' => 'Store at 4°C.',
		'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% sodium azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'chd1-polyclonal-antibody-20',
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		'modified' => '2021-01-06 10:20:50',
		'created' => '2020-12-30 11:59:24',
		'select_label' => '634 - CHD1 Antibody - ChIP-seq Grade (sample size) (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)'
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			'name' => 'CHD1 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div>
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<div class="extra-spaced"></div>
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<div class="extra-spaced"></div>
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<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<td>Fig 1, 2</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div>
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<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p>
</div>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p>
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CHD1 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protei...	Download
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