Polyclonal antibody raised in rabbit against human CHD5 (chromodomain helicase DNA binding protein 5), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the central part of the protein, respectively.
| Lot | A473-002 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 - 1:1,000 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CHD5 (Cat. No. CS-121-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:93,500.
Figure 2. Western blot analysis using the Diagenode antibody directed against CHD5
HeLa cells were transfected with CHD5 and analysed by Western blot. Western blot was performed using 15 to 30 μg nuclear extracts and the Diagenode antibody against human CHD5 (Cat. No. CS-121-100). The antibody was diluted 1:1,000 in TBS-Tween containing 2% milk powder. The position of the protein of interest (expected size: 223 kDa) is indicated on the right; the marker (in kDa) is shown on the left. Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells. Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
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