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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-b.jpg" alt="CHD7 Antibody for ChIP-seq " /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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<li>100% satisfaction guarantee</li>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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'description' => '<p>Polyclonal antibody raised in rabbit against human CHD7 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from the C-terminal part of the protein<sup>1</sup>.</p><p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chip.jpg" alt="CHD7 Antibody ChIP Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-a.jpg" alt="CHD7 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-b.jpg" alt="CHD7 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-c.jpg" alt="CHD7 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-d.jpg" alt="CHD7 Antibody validated in ChIP-seq " /></p>
</div>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-wb.jpg" alt="CHD7 Antibody validated in Western Blot" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-ip.jpg" alt="CHD7 Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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'description' => 'CHD7 (UniProtKB/Swiss-Prot entry Q9P2D1) is a putative transcription regulator which may be involved in 45S precursor rRNA
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'lot' => 'A301-223A3',
'concentration' => '0.2 μg/μl',
'reactivity' => 'Human: positive. Other species: not tested.',
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'application_table' => '<table>
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<th>Suggested dilution</th>
<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
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<tr>
<td>Immunofluorescence</td>
<td>1:500</td>
<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD7 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein<sup>1</sup>.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chip.jpg" alt="CHD7 Antibody ChIP Grade" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-a.jpg" alt="CHD7 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-b.jpg" alt="CHD7 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-c.jpg" alt="CHD7 Antibody for ChIP-seq assay" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-chipseq-d.jpg" alt="CHD7 Antibody validated in ChIP-seq " /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-wb.jpg" alt="CHD7 Antibody validated in Western Blot" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7</strong><br />Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410340-ip.jpg" alt="CHD7 Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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),
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),
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'modified' => '2018-01-03 11:09:44',
'created' => '2018-01-03 11:09:44',
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[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
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'alt' => 'ChIP-seq Grade',
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)
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),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
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(int) 0 => array(
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(int) 2 => array(
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'created' => '2022-08-29 09:56:24',
'ProductsSafetySheet' => array(
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),
(int) 3 => array(
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)
),
(int) 4 => array(
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)
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(int) 5 => array(
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'name' => 'CHD7 Antibody SDS BE fr',
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'url' => 'files/SDS/CHD7/SDS-C15410340-CHD7_Antibody-BE-fr-GHS_1_0.pdf',
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'modified' => '2022-08-29 09:56:24',
'created' => '2022-08-29 09:56:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
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'url' => 'files/SDS/CHD7/SDS-C15410340-CHD7_Antibody-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2022-08-29 09:56:24',
'created' => '2022-08-29 09:56:24',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
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'url' => 'files/SDS/CHD7/SDS-C15410340-CHD7_Antibody-ES-es-GHS_1_0.pdf',
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'created' => '2022-08-29 09:56:24',
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)
)
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(int) 1 => 'US',
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(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
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'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
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'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
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'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'locale' => 'jpn'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
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'name' => 'CHD7 polyclonal antibody',
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'meta_description' => '',
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'created' => '2018-01-03 11:09:44',
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'language' => 'es',
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'countries' => 'ES',
'modified' => '2022-08-29 09:56:24',
'created' => '2022-08-29 09:56:24',
'ProductsSafetySheet' => array(
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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