Polyclonal antibody raised in rabbit against Cre recombinase using a KLH-conjugated synthetic peptide located at the C-terminal part of the protein.
| Lot | A1040-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Western Blotting | 1:500 | Fig 2, (1) |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Cre (cat. No. CS-168-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,900.
Figure 2. Western blot analysis using the Diagenode antibody directed against Cre (1)
Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Cre (cat. No. CS-168-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 38 KD) is indicated on the right.
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