ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A1039-001 |
---|---|
Concentration | not determined |
Species reactivity | Mouse |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:500 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,100.
Datasheet DGCR8 CS-PA027-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: DGCR8 polyclonal antibody (Diagenode Cat# C15310247 Lot# A1039-001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'DGCR8 polyclonal antibody - Pioneer', 'meta_title' => 'DGCR8 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2158', 'antibody_id' => '240', 'name' => 'DGCR8 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>Gy1</strong>, <strong>DGCRK6</strong>, <strong>C22orf12</strong>, <strong>pasha</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DGCR8 (DiGeorge syndrome critical region gene 8) using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310247-Fig1-ELISA.jpg" alt="ELISA" height="339" width="400" /></p> </div> <div class="«" small-8="" columns=""> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,100.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310247', 'old_catalog_number' => 'CS-PA027-100', 'sf_code' => 'C15310247-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'dgcr8-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'DGCR8 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'DGCR8 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 15:30:18', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '240', 'name' => 'DGCR8 polyclonal antibody', 'description' => 'DGCR8 (UniProtKB/Swiss-Prot entry Q8WYQ5) is a component of the microprocessor complex that acts as a RNA- and heme- binding protein and is involved in the initial step of microRNA (miRNA) biogenesis. This microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA- ssRNA junction and directs DROSHA to cleave 11 bp away form the junction. This releases hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'DGCR8 polyclonal antibody - Pioneer', 'meta_title' => 'DGCR8 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2158', 'antibody_id' => '240', 'name' => 'DGCR8 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>Gy1</strong>, <strong>DGCRK6</strong>, <strong>C22orf12</strong>, <strong>pasha</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DGCR8 (DiGeorge syndrome critical region gene 8) using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310247-Fig1-ELISA.jpg" alt="ELISA" height="339" width="400" /></p> </div> <div class="«" small-8="" columns=""> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. 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This microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA- ssRNA junction and directs DROSHA to cleave 11 bp away form the junction. This releases hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DGCR8 (cat. No. CS- PA027-100). The plates were coated with the peptides used for immunization of the rabbit. 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This microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA- ssRNA junction and directs DROSHA to cleave 11 bp away form the junction. This releases hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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