ELISA Enzyme-linked immunosorbent assay. Read more |
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Alternative names: RN3, RNASEN, RNASE3L, RNase III, p241, RANSE3L, ETOHI2
Polyclonal antibody raised in rabbit against mouse DROSHA (drosha, ribonuclease type III) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.
Lot | A1171-004 |
---|---|
Concentration | not determined |
Species reactivity | Mouse |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:500 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,100.
Datasheet DROSHA CS-PA026-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'DROSHA polyclonal antibody - Pioneer', 'meta_title' => 'DROSHA polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2157', 'antibody_id' => '182', 'name' => 'DROSHA polyclonal antibody ', 'description' => '<p>Alternative names: <strong>RN3</strong>, <strong>RNASEN</strong>, <strong>RNASE3L</strong>, <strong>RNase III</strong>, <strong>p241</strong>, <strong>RANSE3L</strong>, <strong>ETOHI2</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DROSHA (drosha, ribonuclease type III) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310246-Fig1-ELISA.jpg" alt="ELISA" height="321" width="400" /></p> </div> <div class="«" small-8="" columns=""> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'DROSHA polyclonal antibody - Pioneer', 'meta_title' => 'DROSHA polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2157', 'antibody_id' => '182', 'name' => 'DROSHA polyclonal antibody ', 'description' => '<p>Alternative names: <strong>RN3</strong>, <strong>RNASEN</strong>, <strong>RNASE3L</strong>, <strong>RNase III</strong>, <strong>p241</strong>, <strong>RANSE3L</strong>, <strong>ETOHI2</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DROSHA (drosha, ribonuclease type III) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310246-Fig1-ELISA.jpg" alt="ELISA" height="321" width="400" /></p> </div> <div class="«" small-8="" columns=""> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'DROSHA polyclonal antibody - Pioneer', 'meta_title' => 'DROSHA polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2157', 'antibody_id' => '182', 'name' => 'DROSHA polyclonal antibody ', 'description' => '<p>Alternative names: <strong>RN3</strong>, <strong>RNASEN</strong>, <strong>RNASE3L</strong>, <strong>RNase III</strong>, <strong>p241</strong>, <strong>RANSE3L</strong>, <strong>ETOHI2</strong></p> <p>Polyclonal antibody raised in rabbit against mouse DROSHA (drosha, ribonuclease type III) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310246-Fig1-ELISA.jpg" alt="ELISA" height="321" width="400" /></p> </div> <div class="«" small-8="" columns=""> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse DROSHA (cat. No. CS- PA026-100). The plates were coated with the peptides used for immunization of the rabbit. 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DROSHA is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within this complex, DROSHA cleaves the 3’ and 5’ strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.', 'clonality' => '', 'isotype' => '', 'lot' => 'A1171-004', 'concentration' => 'not determined', 'reactivity' => 'Mouse', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul> </div> </div> <p></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'mirna-sirna', 'cookies_tag_id' => null, 'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing', 'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode', 'modified' => '2020-09-01 11:42:06', 'created' => '2014-11-12 02:34:56', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '323', 'name' => 'Datasheet DROSHA CS-PA026-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_DROSHA_CS-PA026-100.pdf', 'slug' => 'datasheet-drosha-cs-pa026-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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