Request a quote for a bulk order for EGFP polyclonal antibody . Please fill out the form here below. Your local sales account manager will get in touch with you shortly and send you a quotation based on your requirements.
Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein
| Lot | 001 |
|---|---|
| Concentration | 1.6 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Goat |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Immunofluorescence | 1:800 | Fig 1 |
Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP
A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections.
| Datasheet EGFP pAb-074-050 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: EGFP polyclonal antibody (Diagenode Cat# C15410074 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'EGFP polyclonal antibody - Classic' $meta_title = 'EGFP polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-074-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( 'id' => '2131', 'product_id' => '2235', 'application_id' => '29' ) ) $slugs = array( (int) 0 => 'immunofluorescence' ) $applications = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'locale' => 'jpn' ) $description = '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>' $name = 'IF' $document = array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '278', 'product_id' => '2235', 'document_id' => '620' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'EGFP polyclonal antibody - Classic' $meta_title = 'EGFP polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-074-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( 'id' => '2131', 'product_id' => '2235', 'application_id' => '29' ) ) $slugs = array( (int) 0 => 'immunofluorescence' ) $applications = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'locale' => 'jpn' ) $description = '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>' $name = 'IF' $document = array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '278', 'product_id' => '2235', 'document_id' => '620' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'EGFP polyclonal antibody - Classic' $meta_title = 'EGFP polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-074-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( 'id' => '2131', 'product_id' => '2235', 'application_id' => '29' ) ) $slugs = array( (int) 0 => 'immunofluorescence' ) $applications = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'locale' => 'jpn' ) $description = '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>' $name = 'IF' $document = array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '278', 'product_id' => '2235', 'document_id' => '620' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'EGFP polyclonal antibody - Classic' $meta_title = 'EGFP polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2235', 'antibody_id' => '309', 'name' => 'EGFP polyclonal antibody ', 'description' => '<p>Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410074_fig1.jpg" alt="Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong> Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP</strong><br /> A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/32 µl', 'catalog_number' => 'C15410074', 'old_catalog_number' => 'pAb-074-050', 'sf_code' => 'C15410074-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'egfp-polyclonal-antibody-classic-50-ug-32-ul', 'meta_title' => 'EGFP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'EGFP polyclonal antibody - Classic', 'modified' => '2022-01-05 15:34:39', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '309', 'name' => 'EGFP polyclonal antibody', 'description' => 'The green fluorescent protein (GFP) is a protein originally isolated from jellyfish that fluoresces green when exposed to blue light. GFP is frequently used as a reporter of expression. The GFP gene can be stably introduced into organisms and maintained in their genome throughout breeding. Alternatively, it can be injected locally using a viral vector. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been labelled using GFP as a marker. Enhanced GFP (EGFP) is a mutation of GFP that significantly improves the spectral characteristics of GFP, resulting in increased fluorescence and photostablility, and a shift of the major excitation peak to 488 nm with the peak emission kept at 509 nm. The latter matches the spectral characteristics of commonly used FITC filter sets, making it more practical to use.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.6 µg/µl', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Immunofluorescence</td> <td>1:800</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-09-28 12:08:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '309 - EGFP polyclonal antibody (001 - 1.6 µg/µl - Human - Affinity purified - Goat)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-074-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( 'id' => '2131', 'product_id' => '2235', 'application_id' => '29' ) ) $slugs = array( (int) 0 => 'immunofluorescence' ) $applications = array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'locale' => 'jpn' ) $description = '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>' $name = 'IF' $document = array( 'id' => '620', 'name' => 'Datasheet EGFP pAb-074-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_EGFP_pAb-074-050.pdf', 'slug' => 'datasheet-egfp-pab-074-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '278', 'product_id' => '2235', 'document_id' => '620' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.
