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'description' => '<p><strong>Other names:</strong> ESE3, ESE-3, ESE3B, ESEJ, HEHF</p><p>Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2c.jpg" alt="EHF Antibody for ChIP-seq assay" caption="false" width="800" height="148" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2a.jpg" alt="EHF Antibody ChIP-seq Grade" caption="false" width="800" height="100" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2c.jpg" alt="EHF Antibody for ChIP-seq assay" caption="false" width="800" height="148" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2a.jpg" alt="EHF Antibody ChIP-seq Grade" caption="false" width="800" height="100" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2c.jpg" alt="EHF Antibody for ChIP-seq assay" caption="false" width="800" height="148" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2d.jpg" alt="EHF Antibody validated in ChIP-seq" caption="false" width="800" height="183" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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'name' => 'EHF is a novel regulator of cellular redox metabolism and predictspatient prognosis in HNSCC.',
'authors' => 'Oyelakin A. et al.',
'description' => '<p>Head and Neck Squamous Cell Carcinoma (HNSCC) is a heterogeneous disease with relatively high morbidity and mortality rates. The lack of effective therapies, high recurrence rates and drug resistance driven in part, by tumor heterogeneity, contribute to the poor prognosis for patients diagnosed with this cancer. This problem is further exacerbated by the fact that key regulatory factors contributing to the disease diversity remains largely elusive. Here, we have identified EHF as an important member of the ETS family of transcription factors that is highly expressed in normal oral tissues, but lost during HNSCC progression. Interestingly, HNSCC tumors and cell lines exhibited a dichotomy of high and low EHF expression, and patients whose tumors retained EHF expression showed significantly better prognosis, suggesting a potential tumor suppressive role for EHF. To address this, we have performed gain and loss of function studies and leveraged bulk and single-cell cancer genomic datasets to identify global EHF targets by RNA-sequencing (RNA-seq) and Chromatin Immunoprecipitation and next generation sequencing (ChIP-seq) experiments of HNSCC cell lines. These mechanistic studies have revealed that EHF, acts as a regulator of a broad spectrum of metabolic processes, specifically targeting regulators of redox homeostasis such as NRF2 and SOX2. Our immunostaining results confirm the mutually exclusive expression patterns of EHF and SOX2 in HNSCC tumors and suggest a possible role for these two factors in establishing discrete metabolic states within the tumor microenvironment. Taken together, EHF may serve as a novel prognostic marker for classifying HNSCC patients for actionable and targeted therapeutic intervention.</p>',
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'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35664541',
'doi' => '10.1093/narcan/zcac017',
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410363-elisa.jpg" alt="EHF Antibody ELISA Validation" caption="false" width="432" height="308" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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'description' => '<p>Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</p>',
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'name' => 'EHF is a novel regulator of cellular redox metabolism and predictspatient prognosis in HNSCC.',
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'description' => '<p>Head and Neck Squamous Cell Carcinoma (HNSCC) is a heterogeneous disease with relatively high morbidity and mortality rates. The lack of effective therapies, high recurrence rates and drug resistance driven in part, by tumor heterogeneity, contribute to the poor prognosis for patients diagnosed with this cancer. This problem is further exacerbated by the fact that key regulatory factors contributing to the disease diversity remains largely elusive. Here, we have identified EHF as an important member of the ETS family of transcription factors that is highly expressed in normal oral tissues, but lost during HNSCC progression. Interestingly, HNSCC tumors and cell lines exhibited a dichotomy of high and low EHF expression, and patients whose tumors retained EHF expression showed significantly better prognosis, suggesting a potential tumor suppressive role for EHF. To address this, we have performed gain and loss of function studies and leveraged bulk and single-cell cancer genomic datasets to identify global EHF targets by RNA-sequencing (RNA-seq) and Chromatin Immunoprecipitation and next generation sequencing (ChIP-seq) experiments of HNSCC cell lines. These mechanistic studies have revealed that EHF, acts as a regulator of a broad spectrum of metabolic processes, specifically targeting regulators of redox homeostasis such as NRF2 and SOX2. Our immunostaining results confirm the mutually exclusive expression patterns of EHF and SOX2 in HNSCC tumors and suggest a possible role for these two factors in establishing discrete metabolic states within the tumor microenvironment. Taken together, EHF may serve as a novel prognostic marker for classifying HNSCC patients for actionable and targeted therapeutic intervention.</p>',
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'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35664541',
'doi' => '10.1093/narcan/zcac017',
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'description' => '<p><strong>Other names:</strong> ESE3, ESE-3, ESE3B, ESEJ, HEHF</p>
<p>Polyclonal antibody raised in rabbit against human <strong>EHF (ETS Homologous Factor)</strong>, using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410363-chip.jpg" alt="EHF Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2a.jpg" alt="EHF Antibody ChIP-seq Grade" caption="false" width="800" height="100" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2c.jpg" alt="EHF Antibody for ChIP-seq assay" caption="false" width="800" height="148" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2d.jpg" alt="EHF Antibody validated in ChIP-seq" caption="false" width="800" height="183" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410363-elisa.jpg" alt="EHF Antibody ELISA Validation" caption="false" width="432" height="308" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'description' => '<p>Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</p>',
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'description' => '<p>Head and Neck Squamous Cell Carcinoma (HNSCC) is a heterogeneous disease with relatively high morbidity and mortality rates. The lack of effective therapies, high recurrence rates and drug resistance driven in part, by tumor heterogeneity, contribute to the poor prognosis for patients diagnosed with this cancer. This problem is further exacerbated by the fact that key regulatory factors contributing to the disease diversity remains largely elusive. Here, we have identified EHF as an important member of the ETS family of transcription factors that is highly expressed in normal oral tissues, but lost during HNSCC progression. Interestingly, HNSCC tumors and cell lines exhibited a dichotomy of high and low EHF expression, and patients whose tumors retained EHF expression showed significantly better prognosis, suggesting a potential tumor suppressive role for EHF. To address this, we have performed gain and loss of function studies and leveraged bulk and single-cell cancer genomic datasets to identify global EHF targets by RNA-sequencing (RNA-seq) and Chromatin Immunoprecipitation and next generation sequencing (ChIP-seq) experiments of HNSCC cell lines. These mechanistic studies have revealed that EHF, acts as a regulator of a broad spectrum of metabolic processes, specifically targeting regulators of redox homeostasis such as NRF2 and SOX2. Our immunostaining results confirm the mutually exclusive expression patterns of EHF and SOX2 in HNSCC tumors and suggest a possible role for these two factors in establishing discrete metabolic states within the tumor microenvironment. Taken together, EHF may serve as a novel prognostic marker for classifying HNSCC patients for actionable and targeted therapeutic intervention.</p>',
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'description' => '<p><strong>Other names:</strong> ESE3, ESE-3, ESE3B, ESEJ, HEHF</p><p>Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2c.jpg" alt="EHF Antibody for ChIP-seq assay" caption="false" width="800" height="148" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
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'description' => 'EHF (UniProtKB/Swiss-Prot entry Q9NZC4) belongs to the ETS transcription factor family. This transcriptional activator shows epithelial-specific expression and plays a role in regulating epithelial cell differentiation and proliferation. EHF may also act as a transcriptional repressor of a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. EHF is also a putative tumor suppressor gene and may be involved in carcinogenesis.',
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'lot' => 'A2883P',
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'application_table' => '<table>
<thead>
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<th>References</th>
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<td>ChIP/ChIP-seq*</td>
<td>1 – 2 µg per ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:1,000 - 1:50,000</td>
<td>Fig 3</td>
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<tr>
<td>Western Blotting</td>
<td>Not recommended</td>
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<p></p>
<p><small>*Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 µg per ChIP.</small></p>',
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'description' => '<p><strong>Other names:</strong> ESE3, ESE-3, ESE3B, ESEJ, HEHF</p>
<p>Polyclonal antibody raised in rabbit against human <strong>EHF (ETS Homologous Factor)</strong>, using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410363-chip.jpg" alt="EHF Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.</small></p>
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<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2a.jpg" alt="EHF Antibody ChIP-seq Grade" caption="false" width="800" height="100" /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2b.jpg" alt="EHF Antibody for ChIP-seq" caption="false" width="800" height="218" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2c.jpg" alt="EHF Antibody for ChIP-seq assay" caption="false" width="800" height="148" /></p>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/C15410363-chipseq-2d.jpg" alt="EHF Antibody validated in ChIP-seq" caption="false" width="800" height="183" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF</strong><br />ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410363-elisa.jpg" alt="EHF Antibody ELISA Validation" caption="false" width="432" height="308" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.</small></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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'name' => 'EHF is a novel regulator of cellular redox metabolism and predictspatient prognosis in HNSCC.',
'authors' => 'Oyelakin A. et al.',
'description' => '<p>Head and Neck Squamous Cell Carcinoma (HNSCC) is a heterogeneous disease with relatively high morbidity and mortality rates. The lack of effective therapies, high recurrence rates and drug resistance driven in part, by tumor heterogeneity, contribute to the poor prognosis for patients diagnosed with this cancer. This problem is further exacerbated by the fact that key regulatory factors contributing to the disease diversity remains largely elusive. Here, we have identified EHF as an important member of the ETS family of transcription factors that is highly expressed in normal oral tissues, but lost during HNSCC progression. Interestingly, HNSCC tumors and cell lines exhibited a dichotomy of high and low EHF expression, and patients whose tumors retained EHF expression showed significantly better prognosis, suggesting a potential tumor suppressive role for EHF. To address this, we have performed gain and loss of function studies and leveraged bulk and single-cell cancer genomic datasets to identify global EHF targets by RNA-sequencing (RNA-seq) and Chromatin Immunoprecipitation and next generation sequencing (ChIP-seq) experiments of HNSCC cell lines. These mechanistic studies have revealed that EHF, acts as a regulator of a broad spectrum of metabolic processes, specifically targeting regulators of redox homeostasis such as NRF2 and SOX2. Our immunostaining results confirm the mutually exclusive expression patterns of EHF and SOX2 in HNSCC tumors and suggest a possible role for these two factors in establishing discrete metabolic states within the tumor microenvironment. Taken together, EHF may serve as a novel prognostic marker for classifying HNSCC patients for actionable and targeted therapeutic intervention.</p>',
'date' => '2022-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35664541',
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'modified' => '2023-08-01 13:57:36',
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$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/35664541" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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