Alternative names: GLP1, KMT1D
Polyclonal antibody raised in rabbit against mouse Ehmt1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein.
| Lot | A265-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 – 1:200 | Fig 1 |
| Western Blotting | 1:500 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2c
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.
 How to properly cite our product/service in your workWe strongly recommend using this: EHMT1 polyclonal antibody (Hologic Diagenode Cat# C15310114 Lot# A265-004 ). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
