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EHMT1 polyclonal antibody

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Catalog Number

Format

Price

C15310114
(CS-114-100)

100 µl

$260.00
Bulk order

Alternative names: GLP1, KMT1D

Polyclonal antibody raised in rabbit against mouse Ehmt1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein.

Lot	A265-004
Concentration	not determined
Species reactivity	Human, mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100 – 1:200	Fig 1
Western Blotting	1:500	Fig 2

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2c
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.

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<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => '<p>Alternative names: <strong>GLP1</strong>, <strong>KMT1D</strong></p>
<p>Polyclonal antibody raised in rabbit against mouse Ehmt1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein.</p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310114_fig1.jpg" alt="ELISA" height="238" width="299" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p>
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</div>
<div class="row">
<div class="small-3 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310114_fig2.jpg" alt="Western blot" height="150" width="113" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-9 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2c</strong><br /> Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.</small></p>
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			'name' => 'EHMT1 polyclonal antibody',
			'description' => 'Ehmt1 (UniProt/Swiss-Prot entry Q9H9B1) is a histone methyltransferase which specifically methylates histone H3 on lysine 9. H3K9 methylation represents a marker for epigenetic transcriptional repression. This repression is established by recruiting HP1 proteins to the methylated histone. H3K9 methylation may contribute to silencing of MYC and E2F responsive genes and plays a role in murine embryogenesis.',
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			'lot' => 'A265-004 ',
			'concentration' => 'not determined',
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			'type' => 'Polyclonal',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
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<tbody>
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<td>ELISA</td>
<td>1:100 &ndash; 1:200</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 2</td>
</tr>
</tbody>
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<p>Polyclonal antibody raised in rabbit against mouse Ehmt1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310114_fig1.jpg" alt="ELISA" height="238" width="299" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against JMJD2c</strong><br /> Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against JMJD2c (Cat. No. CS-105-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet Ehmt1 CS-114-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

EHMT1 antibody SDS GB en	Download
EHMT1 antibody SDS US en	Download
EHMT1 antibody SDS DE de	Download
EHMT1 antibody SDS JP ja	Download
EHMT1 antibody SDS BE nl	Download
EHMT1 antibody SDS BE fr	Download
EHMT1 antibody SDS FR fr	Download
EHMT1 antibody SDS ES es	Download

EHMT1 polyclonal antibody

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