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Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).
| Lot | 001 |
|---|---|
| Concentration | 1 µg/µl |
| Species reactivity | Human, mouse, rat, hamster: positive. Other species: not tested. |
| Type | Monoclonal |
| Purity | Purified monoclonal antibody. |
| Host | Mouse |
| Storage Conditions | Store at -20°C. |
| Storage Buffer | PBS containing 50% glycerol, does not contain a preservative. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| RIP | 15 µg per 107 cells | Fig 1 |
| Western blotting | 1:1,000 | Fig 2 |
| IP | 1.5 µg per 1.5x105 cells | Fig 3 |
Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1
Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.
Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1
Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.
| RIP RIP Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| ELAVL1 monoclonal antibody Datasheet DATASHEET Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1). | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: ELAVL1 monoclonal antibody (Diagenode Cat# C15200238 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'ELAVL1 monoclonal antibody', 'product' => array( 'Product' => array( 'id' => '2972', 'antibody_id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200238', 'old_catalog_number' => '', 'sf_code' => 'C15200238-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 28, 2018', 'slug' => 'elavl1-monoclonal-antibody-100-ul', 'meta_title' => 'ELAVL1 monoclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:35:12', 'created' => '2018-06-28 12:00:13', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => 'ELAVL1 (UniProtKB/Swiss-Prot entry Q15717) is a member of the ELAVL RNA-binding protein family. 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Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'ELAVL1 monoclonal antibody', 'product' => array( 'Product' => array( 'id' => '2972', 'antibody_id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. 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Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '28', 'position' => '40', 'parent_id' => '4', 'name' => 'RNA associated proteins', 'description' => '<div class="row"> <div style="text-align: justify;" class="large-12 columns"> <p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'ELAVL1 monoclonal antibody', 'product' => array( 'Product' => array( 'id' => '2972', 'antibody_id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200238', 'old_catalog_number' => '', 'sf_code' => 'C15200238-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 28, 2018', 'slug' => 'elavl1-monoclonal-antibody-100-ul', 'meta_title' => 'ELAVL1 monoclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:35:12', 'created' => '2018-06-28 12:00:13', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => 'ELAVL1 (UniProtKB/Swiss-Prot entry Q15717) is a member of the ELAVL RNA-binding protein family. 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Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'ELAVL1 monoclonal antibody', 'product' => array( 'Product' => array( 'id' => '2972', 'antibody_id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. The immunoprecipitated ELAVL1 protein was subsequently detected by western blot with the ELAVL1 antibody as described above.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200238', 'old_catalog_number' => '', 'sf_code' => 'C15200238-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '0', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'March 28, 2018', 'slug' => 'elavl1-monoclonal-antibody-100-ul', 'meta_title' => 'ELAVL1 monoclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-05 15:35:12', 'created' => '2018-06-28 12:00:13', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => 'ELAVL1 (UniProtKB/Swiss-Prot entry Q15717) is a member of the ELAVL RNA-binding protein family. It selectively binds to poly-U elements and AU-rich elements (AREs) in the 3-UTR of target mRNAs, thereby stabilizing the mRNA. ELAVL1 has been implicated in a variety of biological processes including embryonic stem cells (ESCs) differentiation and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.', 'clonality' => '', 'isotype' => 'IgG2aκ', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => 'Human, mouse, rat, hamster: positive. Other species: not tested.', 'type' => 'Monoclonal', 'purity' => 'Purified monoclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>RIP</td> <td>15 µg per 10<sup>7</sup> cells</td> <td>Fig 1</td> </tr> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> <tr> <td>IP</td> <td>1.5 µg per 1.5x10<sup>5</sup> cells</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C.', 'storage_buffer' => 'PBS containing 50% glycerol, does not contain a preservative.', 'precautions' => 'This product is for research use only. 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Other species: not tested. - Purified monoclonal antibody. - Mouse)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'ELAVL1 monoclonal antibody' $product = array( 'Product' => array( 'id' => '2972', 'antibody_id' => '527', 'name' => 'ELAVL1 monoclonal antibody', 'description' => '<p>Monoclonal antibody raised in mouse against human ELAVL1 (ELAV Like RNA Binding Protein 1).</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-rip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million HeLa cells using 15 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) or with an equal amount of mouse IgG2a, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL1 antibody (upper right). The lower figure shows the gel image for the input, the negative IgG control and the ELAVL1 antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Whole cell extracts from 293T, HeLa, K562, Jurkat, NIH3T3, WR19L, Rat1, PC12 and CHO cells (lanes 1 to 9, respectively) were analysed by Western blot using the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200238-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 3. Immunoprecipitation using the Diagenode monoclonal antibody directed against ELAVL1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 1.5 µg of the Diagenode monoclonal antibody against ELAVL1 (Cat. No. C15200238, lane 3). An equal amount of mouse IgG2a was used as a negative control (lane 2). Lane 1 shows the input. 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