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ELAVL2 polyclonal antibody

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C15410347

100 µg

$380.00
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Polyclonal antibody raised in rabbit against human ELAVL2 (ELAV Like RNA Binding Protein 2), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.

Lot	001
Concentration	1 μg/μl
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C.
Storage Buffer	PBS containing 50% glycerol, does not contain a preservative.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP	15 µg per 10⁷ cells	Fig 1
Western blotting	1:1,000	Fig 2
IP	5 µg per 2.5x10⁵ cells	Fig 3

Validation data

Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2
Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 µg of the Diagenode antibody against ELAVL2 (Cat. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2
Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (Cat. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2
Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 µg of the Diagenode antibody against ELAVL2 (Cat. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.

資料
ELAVL2 polyclonal antibody datasheet
Polyclonal antibody raised in rabbit against human ELAVL2 (ELAV Like RNA Binding Protein 2), usin... Download

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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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$rrbs_service = array(
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	(int) 1 => (int) 1835,
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$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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	'name' => 'IP',
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	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
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	'created' => '2015-07-08 13:46:50',
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$name = 'IP'
$document = array(
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	'name' => 'ELAVL2 polyclonal antibody datasheet',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
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	'meta_keywords' => '',
	'meta_description' => '',
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			'antibody_id' => '530',
			'name' => 'ELAVL2 polyclonal antibody',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410347-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410347-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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<td>Fig 1</td>
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<td>Western blotting</td>
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<td>Fig 2</td>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410347-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410347-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410347-ip.jpg" alt="IP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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<td>Fig 1</td>
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<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
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Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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<td>Fig 1</td>
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<td>Fig 2</td>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410347-rip.jpg" alt="ChIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL2</strong><br />Whole cell extracts from 293T cells transfected with an ELAVL2 expression vector (lane 1) and from human brain tissue (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells transfected with an ELAVL2 expression vector using 5 &micro;g of the Diagenode antibody against ELAVL2 (<span>Cat</span>. No. C15410347, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL2 protein was subsequently detected by western blot with the ELAVL2 antibody as described above.</small></p>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>RIP</td>
<td>15 &micro;g per 10<sup>7</sup> cells</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>IP</td>
<td>5 &micro;g per 2.5x10<sup>5</sup> cells</td>
<td>Fig 3</td>
</tr>
</tbody>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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