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G9a polyclonal antibody

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Catalog Number

Format

Price

C15310096
(CS-096-100)

100 µl

$260.00
Bulk order

Alternative names: EHMT2, BAT8, KMT1C, NG36

Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

Lot	A262-004
Concentration	not determined
Species reactivity	Mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:500 - 1:1,000	Fig 1
Western Blotting	1:1,000	Fig 2, (1)

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse G9a (Cat. No. CS-096-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.</small></p>
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<div class="small-5 columns">
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => '<p>Alternative names: <strong>EHMT2</strong>, <strong>BAT8</strong>, <strong>KMT1C</strong>, <strong>NG36</strong></p>
<p>Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310096_fig1.jpg" alt="ELISA" height="300" width="380" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse G9a (Cat. No. CS-096-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310096_fig2.jpg" alt="Western blot" height="132" width="182" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.</small></p>
</div>
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			'meta_title' => 'G9a polyclonal antibody - Pioneer',
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			'meta_description' => 'G9a polyclonal antibody - Pioneer',
			'modified' => '2022-01-05 15:36:24',
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			'locale' => 'jpn'
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			'id' => '314',
			'name' => 'G9a polyclonal antibody',
			'description' => 'G9a (UniProtKB/Swiss-Prot entry Q96KQ7) is a member of the Set 1 family of histone methyltransferases. It preferentially methylates K9 and K27 of histone H3. Methylation of H3K9 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9a is a part of the E2F6.com-1 complex.',
			'clonality' => '',
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			'lot' => 'A262-004',
			'concentration' => 'not determined',
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			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Pioneer',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:500 - 1:1,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, (1)</td>
</tr>
</tbody>
</table>',
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			'modified' => '2016-08-03 15:20:27',
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<p>Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.</p>',
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse G9a (Cat. No. CS-096-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.</small></p>
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	'modified' => '2016-06-15 11:24:06',
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	'language' => 'es',
	'url' => 'files/SDS/G9a/SDS-C15310096-G9a_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2022-10-03 11:34:45',
	'created' => '2022-10-03 11:34:45',
	'ProductsSafetySheet' => array(
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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			'description' => '<p>Alternative names: <strong>EHMT2</strong>, <strong>BAT8</strong>, <strong>KMT1C</strong>, <strong>NG36</strong></p>
<p>Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.</p>',
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse G9a (Cat. No. CS-096-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.</small></p>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310096_fig2.jpg" alt="Western blot" height="132" width="182" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.</small></p>
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<th>References</th>
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<td>ELISA</td>
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<td>Fig 1</td>
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<td>Western Blotting</td>
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<div class="small-5 columns">
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse G9a (Cat. No. CS-096-100). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.</small></p>
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</div>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310096_fig2.jpg" alt="Western blot" height="132" width="182" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against G9a (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the Diagenode antibody against mouse G9a (Cat. No. CS-096-100), diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet G9a CS-096-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

G9a Antibody SDS GB en	Download
G9a Antibody SDS US en	Download
G9a Antibody SDS DE de	Download
G9a Antibody SDS JP ja	Download
G9a Antibody SDS BE nl	Download
G9a Antibody SDS FR fr	Download
G9a Antibody SDS BE fr	Download
G9a Antibody SDS ES es	Download

G9a polyclonal antibody

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