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Polyclonal antibody raised in mouse against GST (Glutathione-S-Transferase), using a recombinant protein.
| Lot | 002 |
|---|---|
| Concentration | 1.47 μg/μl |
| Species reactivity | |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100,000 - 1:900,000 | |
| Western Blotting | 1:1,000 - 1:10,000 | Fig 1, 2 |
Figure 1. GST antibody western blot results
Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C.
Figure 2. GST antibody western blot results
Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet GST C15200207 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: GST monoclonal antibody (Diagenode Cat# C15200207 Lot# 002). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'GST monoclonal antibody - Classic', 'meta_title' => 'GST monoclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2017', 'antibody_id' => '436', 'name' => 'GST monoclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in mouse against GST (Glutathione-S-Transferase), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB.png" alt="Western Blot" width="80" caption="false" height="354" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 1. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C. </small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB-2.png" alt="Western Blot" width="77" height="192" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 2. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µg', 'catalog_number' => 'C15200207', 'old_catalog_number' => '', 'sf_code' => 'C15200207-D001-000527', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'gst-monoclonal-antibody-classic-500-ug', 'meta_title' => 'GST monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'GST monoclonal antibody - Classic', 'modified' => '2022-01-05 15:36:34', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '436', 'name' => 'GST monoclonal antibody - Classic', 'description' => 'GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.', 'clonality' => '', 'isotype' => '', 'lot' => '002', 'concentration' => '1.47 μg/μl', 'reactivity' => '', 'type' => 'Monoclonal', 'purity' => 'Protein A purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100,000 - 1:900,000</td> <td></td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:10,000</td> <td>Fig 1, 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-08-10 17:56:48', 'created' => '2015-07-30 17:21:03', 'select_label' => '436 - GST monoclonal antibody - Classic (002 - 1.47 μg/μl - - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'GST monoclonal antibody - Classic' $meta_title = 'GST monoclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2017', 'antibody_id' => '436', 'name' => 'GST monoclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in mouse against GST (Glutathione-S-Transferase), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB.png" alt="Western Blot" width="80" caption="false" height="354" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 1. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C. </small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB-2.png" alt="Western Blot" width="77" height="192" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 2. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µg', 'catalog_number' => 'C15200207', 'old_catalog_number' => '', 'sf_code' => 'C15200207-D001-000527', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'gst-monoclonal-antibody-classic-500-ug', 'meta_title' => 'GST monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'GST monoclonal antibody - Classic', 'modified' => '2022-01-05 15:36:34', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '436', 'name' => 'GST monoclonal antibody - Classic', 'description' => 'GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.', 'clonality' => '', 'isotype' => '', 'lot' => '002', 'concentration' => '1.47 μg/μl', 'reactivity' => '', 'type' => 'Monoclonal', 'purity' => 'Protein A purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100,000 - 1:900,000</td> <td></td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:10,000</td> <td>Fig 1, 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '32', 'position' => '50', 'parent_id' => '4', 'name' => 'Epitope tags', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'protein-tag', 'cookies_tag_id' => null, 'meta_keywords' => 'Protein tag,Polyclonal antibody,Monoclonal Protein tag antibodies', 'meta_description' => 'Diagenode offers Polyclonal and Monoclonal Protein tag Antibodies', 'meta_title' => 'Polyclonal and Monoclonal Protein tag Antibodies | Diagenode', 'modified' => '2019-07-04 16:02:49', 'created' => '2015-05-09 11:35:10', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( 'id' => '3729', 'product_id' => '2017', 'application_id' => '19' ) ) $slugs = array( (int) 0 => 'western-blot-antibodies' ) $applications = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'jpn' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1874', 'product_id' => '2017', 'document_id' => '38' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'GST monoclonal antibody - Classic', 'meta_title' => 'GST monoclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2017', 'antibody_id' => '436', 'name' => 'GST monoclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in mouse against GST (Glutathione-S-Transferase), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB.png" alt="Western Blot" width="80" caption="false" height="354" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 1. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C. </small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB-2.png" alt="Western Blot" width="77" height="192" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 2. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µg', 'catalog_number' => 'C15200207', 'old_catalog_number' => '', 'sf_code' => 'C15200207-D001-000527', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'gst-monoclonal-antibody-classic-500-ug', 'meta_title' => 'GST monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'GST monoclonal antibody - Classic', 'modified' => '2022-01-05 15:36:34', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '436', 'name' => 'GST monoclonal antibody - Classic', 'description' => 'GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. 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GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. 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GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. 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Secondary antibody: IRDye800TM goat anti-mouse secondary antibody at 1:5,000 for 45 min at RT. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µg', 'catalog_number' => 'C15200207', 'old_catalog_number' => '', 'sf_code' => 'C15200207-D001-000527', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'gst-monoclonal-antibody-classic-500-ug', 'meta_title' => 'GST monoclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'GST monoclonal antibody - Classic', 'modified' => '2022-01-05 15:36:34', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '436', 'name' => 'GST monoclonal antibody - Classic', 'description' => 'GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. 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GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: molecular weight marker. Lane 2: Mouse- anti-GST monoclonal antibody (blue), Rabbit anti-Transferrin, and Goat-anti-Alpha- 1-Anti-Trypsin were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 1 μg per lane. Primary antibody: Each primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 overnight at 4°C. </small></p> </div> </div> <div class="row"> <div class="small-2 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200207-WB-2.png" alt="Western Blot" width="77" height="192" caption="false" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-10 columns"> <p><small><strong> Figure 2. GST antibody western blot results </strong><br />Western Blot of GST antibody. Lane 1: GST recombinant protein. Lane 2: lysate of HeLa cells expressing recombinant GST protein. Load: 0.1 μg per lane. Primary antibody used at 1.0 μg/ml for 1 h at room temperature. 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