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| DB Dot blotting Read more |
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Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Dot Blotting | 1:2,000 | Fig 2 |
| Western Blotting | 1:200 | Fig 3 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p
To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest.
Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p
HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. No. MAb-186-050) diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
| Datasheet H2BS14p MAb-186-050 DATASHEET Datasheet description | Download |
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Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H2Bs14p monoclonal antibody - Pioneer', 'meta_title' => 'H2Bs14p monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2003', 'antibody_id' => '127', 'name' => 'H2Bs14p monoclonal antibody ', 'description' => '<p>Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_DB.jpg" alt="Dot blot" width="224" height="287" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_ELISA.jpg" alt="ELISA" width="378" height="340" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p</strong><br />To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. 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No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:18:29', 'created' => '0000-00-00 00:00:00', 'select_label' => '127 - H2Bs14p monoclonal antibody (001 - 1.0 µg/µl - Human - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '111', 'position' => '40', 'parent_id' => '4', 'name' => 'Histone antibodies', 'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H2Bs14p monoclonal antibody - Pioneer', 'meta_title' => 'H2Bs14p monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2003', 'antibody_id' => '127', 'name' => 'H2Bs14p monoclonal antibody ', 'description' => '<p>Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_DB.jpg" alt="Dot blot" width="224" height="287" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_ELISA.jpg" alt="ELISA" width="378" height="340" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p</strong><br />To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. 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A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H2Bs14p monoclonal antibody - Pioneer', 'meta_title' => 'H2Bs14p monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2003', 'antibody_id' => '127', 'name' => 'H2Bs14p monoclonal antibody ', 'description' => '<p>Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_DB.jpg" alt="Dot blot" width="224" height="287" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_ELISA.jpg" alt="ELISA" width="378" height="340" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p</strong><br />To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. No. MAb-186-050) diluted 1:200 in TBS-Tween containing 5% skimmed milk. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_ELISA.jpg" alt="ELISA" width="378" height="340" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p</strong><br />To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. 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They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:18:29', 'created' => '0000-00-00 00:00:00', 'select_label' => '127 - H2Bs14p monoclonal antibody (001 - 1.0 µg/µl - Human - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '111', 'position' => '40', 'parent_id' => '4', 'name' => 'Histone antibodies', 'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '731', 'name' => 'Datasheet H2BS14p MAb-186-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H2BS14p_MAb-186-050.pdf', 'slug' => 'datasheet-h2bs14p-mab-186-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => 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4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(MAb-186-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' 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=> '2020-09-22 11:50:38', 'ProductsSafetySheet' => array( 'id' => '1427', 'product_id' => '2003', 'safety_sheet_id' => '788' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H2Bs14p monoclonal antibody - Pioneer', 'meta_title' => 'H2Bs14p monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2003', 'antibody_id' => '127', 'name' => 'H2Bs14p monoclonal antibody ', 'description' => '<p>Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_DB.jpg" alt="Dot blot" width="224" height="287" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_ELISA.jpg" alt="ELISA" width="378" height="340" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p</strong><br />To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. No. MAb-186-050) diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 μg/50 μl', 'catalog_number' => 'C15200186', 'old_catalog_number' => 'MAb-186-050', 'sf_code' => 'C15200186-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h2bs14p-monoclonal-antibody-pioneer-50-mg-50-ml', 'meta_title' => 'H2Bs14p monoclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'H2Bs14p monoclonal antibody - Pioneer', 'modified' => '2022-01-05 15:40:03', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '127', 'name' => 'H2Bs14p monoclonal antibody', 'description' => 'Histones are present in the chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Protein A purified', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100</td> <td>Fig 1</td> </tr> <tr> <td>Dot Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:200</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:18:29', 'created' => '0000-00-00 00:00:00', 'select_label' => '127 - H2Bs14p monoclonal antibody (001 - 1.0 µg/µl - Human - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'H2Bs14p monoclonal antibody - Pioneer' $meta_title = 'H2Bs14p monoclonal antibody - Pioneer' $product = array( 'Product' => array( 'id' => '2003', 'antibody_id' => '127', 'name' => 'H2Bs14p monoclonal antibody ', 'description' => '<p>Monoclonal antibody raised in mouse against histone H2B containing the phosphorylated Serine 14 (H2BS14p), using a KLH-conjugated synthetic peptide.</p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_DB.jpg" alt="Dot blot" width="224" height="287" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H2BS14p (Cat. No. MAb-186-050).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_ELISA.jpg" alt="ELISA" width="378" height="340" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody against H2BS14p</strong><br />To test the cross reactivity of the Diagenode antibody against H2BS14p (Cat. No. MAb-186-050), a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure 2 shows a high specificity of the antibody for the modification of interest. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/MAb-186-050_WB.jpg" alt="Western blot" width="141" height="169" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against H2BS14p</strong><br />HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 μg of histone extracts of these cells were analysed by Western blot using the Diagenode antibody against H2BS14p (cat. No. MAb-186-050) diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 μg/50 μl', 'catalog_number' => 'C15200186', 'old_catalog_number' => 'MAb-186-050', 'sf_code' => 'C15200186-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h2bs14p-monoclonal-antibody-pioneer-50-mg-50-ml', 'meta_title' => 'H2Bs14p monoclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'H2Bs14p monoclonal antibody - Pioneer', 'modified' => '2022-01-05 15:40:03', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '127', 'name' => 'H2Bs14p monoclonal antibody', 'description' => 'Histones are present in the chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Protein A purified', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100</td> <td>Fig 1</td> </tr> <tr> <td>Dot Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:200</td> <td>Fig 3</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:18:29', 'created' => '0000-00-00 00:00:00', 'select_label' => '127 - H2Bs14p monoclonal antibody (001 - 1.0 µg/µl - Human - Protein A purified - Mouse)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '111', 'position' => '40', 'parent_id' => '4', 'name' => 'Histone antibodies', 'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '731', 'name' => 'Datasheet H2BS14p MAb-186-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H2BS14p_MAb-186-050.pdf', 'slug' => 'datasheet-h2bs14p-mab-186-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => 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'de', 'url' => 'files/SDS/H2BS14p/SDS-C15200186-H2BS14p_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-09-22 11:50:09', 'created' => '2020-09-22 11:50:09', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '791', 'name' => 'H2BS14p antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/H2BS14p/SDS-C15200186-H2BS14p_Antibody-JP-ja-GHS_3_0.pdf', 'countries' => 'JP', 'modified' => '2020-09-22 11:52:00', 'created' => '2020-09-22 11:52:00', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '786', 'name' => 'H2BS14p antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/H2BS14p/SDS-C15200186-H2BS14p_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-09-22 11:49:40', 'created' => '2020-09-22 11:49:40', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '785', 'name' => 'H2BS14p antibody SDS BE fr', 'language' => 'fr', 'url' => 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4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(MAb-186-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( 'id' => '2274', 'product_id' => '2003', 'application_id' => '28' ) ) $slugs = array( (int) 0 => 'dot-blotting' ) $applications = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'locale' => 'jpn' ) $description = '<p>Dot blotting</p>' $name = 'DB' $document = array( 'id' => '731', 'name' => 'Datasheet H2BS14p MAb-186-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H2BS14p_MAb-186-050.pdf', 'slug' => 'datasheet-h2bs14p-mab-186-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '330', 'product_id' => '2003', 'document_id' => '731' ) ) $sds = array( 'id' => '788', 'name' => 'H2BS14p antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/H2BS14p/SDS-C15200186-H2BS14p_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-09-22 11:50:38', 'created' => '2020-09-22 11:50:38', 'ProductsSafetySheet' => array( 'id' => '1427', 'product_id' => '2003', 'safety_sheet_id' => '788' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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