| Datasheet H3K27me2 sp046 DATASHEET Datasheet description | Download |
| Datasheet H3K27me2 pAb-046-050 DATASHEET Datasheet description | Download |
Synthetic peptide derived from the region of human histone H3 containing the dimethylated lysine 27.
This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K27me2 (Cat. No. pAb-046-050). The same peptide can be used to block this antibody in negative control experiments.
For more information about H3K27me2: see the Technical Data Sheet of the corresponding Diagenode antibody.
| Datasheet H3K27me2 sp046 DATASHEET Datasheet description | Download |
| Datasheet H3K27me2 pAb-046-050 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: H3K27me2 peptide (Diagenode Cat# C16000046). Click here to copy to clipboard. Using our products in your publication? Let us know! |
PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across thezygote genome. |
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In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( 'id' => '5506', 'product_id' => '2443', 'publication_id' => '4211' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33311485" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-046-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '356', 'name' => 'Datasheet H3K27me2 pAb-046-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K27me2_pAb-046-050.pdf', 'slug' => 'datasheet-h3k27me2-pab-046-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '356', 'product_id' => '2443', 'document_id' => '356' ) ) $publication = array( 'id' => '4211', 'name' => 'PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across thezygote genome.', 'authors' => 'Meng T-G et al.', 'description' => '<p>The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( 'id' => '5506', 'product_id' => '2443', 'publication_id' => '4211' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33311485" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H3K27me2 peptide', 'meta_title' => 'H3K27me2 peptide', 'product' => array( 'Product' => array( 'id' => '2443', 'antibody_id' => '0', 'name' => 'H3K27me2 peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the dimethylated lysine 27. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K27me2 (Cat. 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H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-046-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '356', 'name' => 'Datasheet H3K27me2 pAb-046-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K27me2_pAb-046-050.pdf', 'slug' => 'datasheet-h3k27me2-pab-046-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '356', 'product_id' => '2443', 'document_id' => '356' ) ) $publication = array( 'id' => '4211', 'name' => 'PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across thezygote genome.', 'authors' => 'Meng T-G et al.', 'description' => '<p>The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( 'id' => '5506', 'product_id' => '2443', 'publication_id' => '4211' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33311485" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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The same peptide can be used to block this antibody in negative control experiments. </span><br /><br /><span>For more information about H3K27me2: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000046', 'old_catalog_number' => 'sp-046-050', 'sf_code' => 'C16000046-D001-000581', 'type' => 'FRE', 'search_order' => '01-Accessory', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k27me2-peptide-50-ug-50-ul', 'meta_title' => 'H3K27me2 peptide', 'meta_keywords' => '', 'meta_description' => 'H3K27me2 peptide', 'modified' => '2015-07-17 11:44:02', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '206', 'name' => 'Datasheet H3K27me2 sp046', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/peptides/Datasheet_H3K27me2_sp046.pdf', 'slug' => 'datasheet-h3k27me2-sp046', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '356', 'name' => 'Datasheet H3K27me2 pAb-046-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K27me2_pAb-046-050.pdf', 'slug' => 'datasheet-h3k27me2-pab-046-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4211', 'name' => 'PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across thezygote genome.', 'authors' => 'Meng T-G et al.', 'description' => '<p>The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-046-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '356', 'name' => 'Datasheet H3K27me2 pAb-046-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K27me2_pAb-046-050.pdf', 'slug' => 'datasheet-h3k27me2-pab-046-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '356', 'product_id' => '2443', 'document_id' => '356' ) ) $publication = array( 'id' => '4211', 'name' => 'PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across thezygote genome.', 'authors' => 'Meng T-G et al.', 'description' => '<p>The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes.</p>', 'date' => '2020-12-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33311485', 'doi' => '10.1038/s41467-020-20242-9', 'modified' => '2022-01-13 15:07:22', 'created' => '2021-12-06 15:53:19', 'ProductsPublication' => array( 'id' => '5506', 'product_id' => '2443', 'publication_id' => '4211' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33311485" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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