| Datasheet H3K9acS10p sp102 DATASHEET Datasheet description | Download |
| Datasheet H3K9acS10p CS-102-100 DATASHEET Datasheet description | Download |
Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10.
This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.
| Datasheet H3K9acS10p sp102 DATASHEET Datasheet description | Download |
| Datasheet H3K9acS10p CS-102-100 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: H3K9acS10p peptide (Diagenode Cat# C16000102). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'meta_title' => 'H3K9acS10p peptide', 'product' => array( 'Product' => array( 'id' => '2472', 'antibody_id' => '0', 'name' => 'H3K9acS10p peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000102', 'old_catalog_number' => 'sp-102-050', 'sf_code' => 'C16000102-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-peptide-50-ug-50-ul', 'meta_title' => 'H3K9acS10p peptide', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'modified' => '2015-07-20 10:55:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'H3K9acS10p peptide' $meta_title = 'H3K9acS10p peptide' $product = array( 'Product' => array( 'id' => '2472', 'antibody_id' => '0', 'name' => 'H3K9acS10p peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000102', 'old_catalog_number' => 'sp-102-050', 'sf_code' => 'C16000102-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-peptide-50-ug-50-ul', 'meta_title' => 'H3K9acS10p peptide', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'modified' => '2015-07-20 10:55:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. Batch-specific data available on the website. ', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '187', 'name' => 'Datasheet H3K9acS10p sp102', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/peptides/Datasheet_H3K9acS10p_sp102.pdf', 'slug' => 'datasheet-h3k9acs10p-sp102', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/h3k9acs10p-polyclonal-antibody-classic-100-ul"><img src="/img/product/antibodies/ab-chip-icon.png" alt="Antibody ChIP icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15310102</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2093" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2093" id="CartAdd/2093Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2093" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>H3K9acS10p Antibody個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="h3k9acs10p-polyclonal-antibody-classic-100-ul" data-reveal-id="cartModal-2093" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">H3K9acS10p polyclonal antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. 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', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '1161', 'product_id' => '2472', 'related_id' => '2093' ), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-102-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '448', 'product_id' => '2472', 'document_id' => '734' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'meta_title' => 'H3K9acS10p peptide', 'product' => array( 'Product' => array( 'id' => '2472', 'antibody_id' => '0', 'name' => 'H3K9acS10p peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000102', 'old_catalog_number' => 'sp-102-050', 'sf_code' => 'C16000102-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-peptide-50-ug-50-ul', 'meta_title' => 'H3K9acS10p peptide', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'modified' => '2015-07-20 10:55:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'H3K9acS10p peptide' $meta_title = 'H3K9acS10p peptide' $product = array( 'Product' => array( 'id' => '2472', 'antibody_id' => '0', 'name' => 'H3K9acS10p peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000102', 'old_catalog_number' => 'sp-102-050', 'sf_code' => 'C16000102-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-peptide-50-ug-50-ul', 'meta_title' => 'H3K9acS10p peptide', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'modified' => '2015-07-20 10:55:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. Batch-specific data available on the website. ', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '187', 'name' => 'Datasheet H3K9acS10p sp102', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/peptides/Datasheet_H3K9acS10p_sp102.pdf', 'slug' => 'datasheet-h3k9acs10p-sp102', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/h3k9acs10p-polyclonal-antibody-classic-100-ul"><img src="/img/product/antibodies/ab-chip-icon.png" alt="Antibody ChIP icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15310102</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2093" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2093" id="CartAdd/2093Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2093" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>H3K9acS10p Antibody個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="h3k9acs10p-polyclonal-antibody-classic-100-ul" data-reveal-id="cartModal-2093" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">H3K9acS10p polyclonal antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. 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', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '1161', 'product_id' => '2472', 'related_id' => '2093' ), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-102-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '448', 'product_id' => '2472', 'document_id' => '734' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'meta_title' => 'H3K9acS10p peptide', 'product' => array( 'Product' => array( 'id' => '2472', 'antibody_id' => '0', 'name' => 'H3K9acS10p peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000102', 'old_catalog_number' => 'sp-102-050', 'sf_code' => 'C16000102-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-peptide-50-ug-50-ul', 'meta_title' => 'H3K9acS10p peptide', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'modified' => '2015-07-20 10:55:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'H3K9acS10p peptide' $meta_title = 'H3K9acS10p peptide' $product = array( 'Product' => array( 'id' => '2472', 'antibody_id' => '0', 'name' => 'H3K9acS10p peptide', 'description' => '<p><span>Synthetic peptide derived from the region of human histone H3 containing the acetylated lysine; 9 and the phosphorylated serine 10. </span><br /><span>This peptide was used as immunogen to raise the ChIP-grade Diagenode antibody directed against H3K9acS10p (Cat. No. CS-102-100). The same peptide can be used to block this antibody in negative control experiments. For more information about H3K9acS10p: see the Technical Data Sheet of the corresponding Diagenode antibody.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/50 µl', 'catalog_number' => 'C16000102', 'old_catalog_number' => 'sp-102-050', 'sf_code' => 'C16000102-D001-000581', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '90', 'price_USD' => '115', 'price_GBP' => '80', 'price_JPY' => '14100', 'price_CNY' => '', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-peptide-50-ug-50-ul', 'meta_title' => 'H3K9acS10p peptide', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p peptide', 'modified' => '2015-07-20 10:55:43', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. 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', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '187', 'name' => 'Datasheet H3K9acS10p sp102', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/peptides/Datasheet_H3K9acS10p_sp102.pdf', 'slug' => 'datasheet-h3k9acs10p-sp102', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' 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style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2093" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2093" id="CartAdd/2093Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2093" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>H3K9acS10p Antibody個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="h3k9acs10p-polyclonal-antibody-classic-100-ul" data-reveal-id="cartModal-2093" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">H3K9acS10p polyclonal antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. 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ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. Batch-specific data available on the website. ', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '93', 'position' => '3', 'parent_id' => '36', 'name' => 'Peptides', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'peptides', 'cookies_tag_id' => null, 'meta_keywords' => 'Peptides ,Proteins ', 'meta_description' => 'Diagenode offers wide range of Peptides ', 'meta_title' => 'Peptides & Proteins | Diagenode', 'modified' => '2016-01-21 15:58:23', 'created' => '2015-09-21 14:44:25', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '187', 'name' => 'Datasheet H3K9acS10p sp102', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/peptides/Datasheet_H3K9acS10p_sp102.pdf', 'slug' => 'datasheet-h3k9acs10p-sp102', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/h3k9acs10p-polyclonal-antibody-classic-100-ul"><img src="/img/product/antibodies/ab-chip-icon.png" alt="Antibody ChIP icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C15310102</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2093" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2093" id="CartAdd/2093Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2093" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>H3K9acS10p Antibody個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('H3K9acS10p Antibody', 'C15310102', '380', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="h3k9acs10p-polyclonal-antibody-classic-100-ul" data-reveal-id="cartModal-2093" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">H3K9acS10p polyclonal antibody </h6> </div> </div> </li> ' $related = array( 'id' => '2093', 'antibody_id' => '56', 'name' => 'H3K9acS10p Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>histone H3</strong> containing the <strong>acetylated lysine 9 and the phosphorylated serine 10</strong> (<strong>H3K9acS10p</strong>), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_chip.png" alt="H3K9acS10p Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="396" /></p> <p></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against H3K9acS10p</strong><br />ChIP assays were performed using human osteosarcoma (U2OS) cells, the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) and optimized PCR primer sets for qPCR. Chromatin was sheared with the Diagenode “Shearing ChIP” kit (Cat. No. kch-redmod-100). ChIP was performed with the “OneDay ChIP” kit (Cat. No. kch-oneDIP-060), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_ELISA.png" alt="H3K9acS10p Antibody ELISA validation" caption="false" width="400" height="316" /></p> <p class="text-center"></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody against human H3K9acS10p (Cat. No. CS-102-100). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:89,000.</small></p> </div> </div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="" spacer=""></div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_DotBlot.png" alt="H3K9acS10p Antibody validated in Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" width="700" height="310" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 3. Cross reactivity test using the Diagenode antibody directed against H3K9acS10p</strong><br />A Dot Blot analysis was performed to test the cross reactivity of the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) with peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 3 shows a high specificity of the antibody for the double modification.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_WB.png" alt="H3K9acS10p Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="186" height="223" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against H3K9acS10p</strong><br />Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode antibody against H3K9acS10p (Cat. No. CS-102-100) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310102_IF.png" alt="H3K9acS10p Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" width="400" height="99" /></p> </div> <div class="small-6 columns"> <p><small> <strong>Figure 5. Immunofluorescence using the Diagenode antibody directed against H3K9acS10p</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9acS10p (cat. No. C15310102) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10p antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of K9 and phosphorylation of S10 of histone H3 are associated with active gene transcription</p>', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310102', 'old_catalog_number' => 'CS-102-100', 'sf_code' => 'C15310102-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '380', 'price_USD' => '380', 'price_GBP' => '340', 'price_JPY' => '59525', 'price_CNY' => '', 'price_AUD' => '950', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k9acs10p-polyclonal-antibody-classic-100-ul', 'meta_title' => 'H3K9acS10p Antibody - ChIP Grade (C15310102) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K9acS10p (Histone H3 acetylated at lysine 9 and phosphorylated at serine 10) Polyclonal Antibody validated in ChIP-qPCR, ELISA, WB, DB and IF. Batch-specific data available on the website. ', 'modified' => '2021-12-23 11:34:43', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '1161', 'product_id' => '2472', 'related_id' => '2093' ), 'Image' => array( (int) 0 => array( 'id' => '1779', 'name' => 'product/antibodies/ab-chip-icon.png', 'alt' => 'Antibody ChIP icon', 'modified' => '2020-08-12 11:52:55', 'created' => '2018-03-15 15:52:35', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(sp-102-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '734', 'name' => 'Datasheet H3K9acS10p CS-102-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K9acS10p_CS-102-100.pdf', 'slug' => 'datasheet-h3k9acs10p-cs-102-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:45', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '448', 'product_id' => '2472', 'document_id' => '734' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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