H3T6p polyclonal antibody

Figure 1. H3T6p antibody IF results
Immunofluorescence of H3T6p antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T6p is nuclear and chromosomal. Staining: H3T6p is expressed in green and the nuclei are counterstained with DAPI (blue).

Figure 2. H3T6p antibody Western blot results
Western Blot of H3T6p antibody. 30 μg C. elegans embryo lysate. Primary antibody incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

Figure 3. H3T6p antibody Western blot results
Western Blot of H3T6p antibody on 30 μg HeLa histone extracts. Primary antibody: incubated at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

Figure 4. H3T6p antibody dot blot results
Dot Blot of H3T6p antibody. Lane 1: H3T6un. Lane 2: H3T6p. Load: 1, 0.3, 0.1, 0.03 picomoles of peptide. Primary antibody used at a 1:1000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.