H4K20me2 polyclonal antibody

Figure 1. ChIP
Chromatin Immunoprecipitation of H4K20me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H4K20me2 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.

Figure 2. Immunofluorescence
Immunofluorescence of H4K20me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4K20me2 is nuclear and chromosomal. Staining: Histone H4K20me2 is expressed in green, nuclei are counterstained with DAPI (blue).

Figure 3. Western Blot
Western Blot of H4K20me2 antibody. 30 μg C. elegans embryo lysate. Primary antibody used at a 1:2000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.

Figure 4. Western Blot
Western Blot of H4K20me2 antibody. 30 μg HeLa histone extracts. Primary antibody at a 1:2000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.