Alternative names: HD3, RPD3, RPD3-2, SMAP45
Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein A purified monoclonal antibody |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Immunofluorescence | 1:500 | Fig 1 |
Figure 1. Immunofluorescence using the Diagenode monoclonal antibody directed against HDAC3
HeLa cells were stained with the Diagenode antibody against HDAC3 (Cat. No. C15200145) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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