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Hira polyclonal antibody

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Catalog Number

Format

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C15310097
(CS-097-100)

100 µl

$260.00
Bulk order

Alternative names: HIR, DGCR1, TUP1, TUPLE1

Polyclonal antibody raised in rabbit against mouse Hira (histone cell cycle regulation defective homolog A), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.

Lot	A367-004
Concentration	not determined
Species reactivity	Mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:500 - 1:200	Fig 1
Western Blotting	1:1,000	Fig 2, (1)

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.

Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.

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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.</small></p>
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<td>Fig 1</td>
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<div class="small-6 columns">
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<p><small><strong>Figure 1. Determination of the titer</strong> <br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.</small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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			'antibody_id' => '263',
			'name' => 'Hira polyclonal antibody ',
			'description' => '<p><span>Alternative names: <strong>HIR</strong>, <strong>DGCR1</strong>, <strong>TUP1</strong>, <strong>TUPLE1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against mouse Hira (histone cell cycle regulation defective homolog A), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310097_fig1.jpg" alt="ELISA" height="296" width="371" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong> <br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310097_fig2.jpg" alt="Western blot" height="134" width="164" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.</small></p>
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			'format' => '100 µl',
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			'meta_title' => 'Hira polyclonal antibody - Pioneer',
			'meta_keywords' => '',
			'meta_description' => 'Hira polyclonal antibody - Pioneer',
			'modified' => '2022-01-05 15:44:28',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
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		'Antibody' => array(
			'host' => '*****',
			'id' => '263',
			'name' => 'Hira polyclonal antibody',
			'description' => 'Hira (UniProtKB/Swiss-Prot entry P54198) is a histone chaperone that cooperates with ASF1A to promote replication-independent chromatin assembly. It is important for histone regulation and is required for the periodic repression of histone gene transcription during the cell cycle. Hira plays an important role in the formation of transcriptionally silent senescence-associated heterochromatic foci (SAHF). SAHF, which contain heterochromatin proteins such as HP1, are believed to repress expression of proliferation-promoting genes, leading to the irreversible cell cycle changes that occur in senescent cells. Interaction between HIRA and ASF1A is the rate limiting step in the formation of SAHF. Hira is also thought to be involved in certain haploinsufficiency syndromes such as DiGeorge syndrome. Insufficient production of Hira protein may disrupt normal embryonic development.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A367-004 ',
			'concentration' => 'not determined',
			'reactivity' => 'Mouse',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum',
			'classification' => 'Pioneer',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tr>
<td>ELISA</td>
<td>1:500 - 1:200</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, (1)</td>
</tr>
</tbody>
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<p><small><strong>Figure 1. Determination of the titer</strong> <br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.</small></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.</small></p>
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	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
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	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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$sds = array(
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	'name' => 'Hira Antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/Hira/SDS-C15310097-Hira_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2022-10-03 11:27:45',
	'created' => '2022-10-03 11:27:45',
	'ProductsSafetySheet' => array(
		'id' => '4428',
		'product_id' => '2088',
		'safety_sheet_id' => '2620'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
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	'meta_keywords' => '',
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	'meta_title' => 'Hira polyclonal antibody - Pioneer',
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<p><small><strong>Figure 1. Determination of the titer</strong> <br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.</small></p>
</div>
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<div class="small-6 columns">
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<div class="small-6 columns">
<p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.</small></p>
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<th>References</th>
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<td>Fig 1</td>
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<td>Fig 2, (1)</td>
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<p><small><strong>Figure 1. Determination of the titer</strong> <br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310097_fig2.jpg" alt="Western blot" height="134" width="164" /></p>
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<p><small><strong> Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)</strong> <br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet Hira CS-097-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

Hira Antibody SDS GB en	Download
Hira Antibody SDS US en	Download
Hira Antibody SDS DE de	Download
Hira Antibody SDS JP ja	Download
Hira Antibody SDS BE nl	Download
Hira Antibody SDS BE fr	Download
Hira Antibody SDS FR fr	Download
Hira Antibody SDS ES es	Download

Hira polyclonal antibody

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