Alternative names: HIR, DGCR1, TUP1, TUPLE1
Polyclonal antibody raised in rabbit against mouse Hira (histone cell cycle regulation defective homolog A), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.
| Lot | A367-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 - 1:200 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2, (1) |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Hira (Cat. No. CS-097-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.
Figure 2. Western blot analysis using the Diagenode antibody directed against Hira (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Hira (Cat. No. CS-097-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.
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