ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A511-002 |
---|---|
Concentration | not determined |
Species reactivity | Human |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:1,000 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human HMG2L1 (cat. No. CS-PA002-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:34,500.
Datasheet HMG2L1 CS-PA002-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: HMG2L1 polyclonal antibody (Diagenode Cat# C15310222 Lot# A511-002). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human HMG2L1 (cat. No. CS-PA002-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human HMG2L1 (cat. No. CS-PA002-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human HMG2L1 (cat. No. CS-PA002-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human HMG2L1 (cat. No. CS-PA002-100). The plates were coated with the peptides used for immunization of the rabbit. 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Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human HMG2L1 (cat. No. CS-PA002-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:34,500.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310222', 'old_catalog_number' => 'CS-PA002-100', 'sf_code' => 'C15310222-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'hmg2l1-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'HMG2L1 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'HMG2L1 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 15:44:38', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '189', 'name' => 'HMG2L1 polyclonal antibody', 'description' => 'HMG2L1 (UniProtKB/Swiss-Prot entry Q9UGU5) is a negative regulator of Wnt/beta-catenin signaling during development.', 'clonality' => '', 'isotype' => '', 'lot' => 'A511-002', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table> <p><small><sup>*</sup> The optimal dilution for other applications should be determined by the end user. 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