| Datasheet Human GAPDH pp1001 DATASHEET Datasheet description | Download |
These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).
| Datasheet Human GAPDH pp1001 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Human GAPDH promoter (TATA-box) primer pair (Diagenode Cat# C17011001-50). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'product' => array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human GAPDH promoter (TATA-box) primer pair' $meta_title = 'Human GAPDH promoter (TATA-box) primer pair' $product = array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ) ), 'Group' => array( 'Group' => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ), 'Master' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) ) $pro = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1001-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '602', 'product_id' => '2513', 'document_id' => '234' ) ) $publication = array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( 'id' => '1319', 'product_id' => '2513', 'publication_id' => '2947' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26559844" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'product' => array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human GAPDH promoter (TATA-box) primer pair' $meta_title = 'Human GAPDH promoter (TATA-box) primer pair' $product = array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ) ), 'Group' => array( 'Group' => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ), 'Master' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) ) $pro = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1001-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '602', 'product_id' => '2513', 'document_id' => '234' ) ) $publication = array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( 'id' => '1319', 'product_id' => '2513', 'publication_id' => '2947' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26559844" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'product' => array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human GAPDH promoter (TATA-box) primer pair' $meta_title = 'Human GAPDH promoter (TATA-box) primer pair' $product = array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ) ), 'Group' => array( 'Group' => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ), 'Master' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) ) $pro = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1001-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '602', 'product_id' => '2513', 'document_id' => '234' ) ) $publication = array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( 'id' => '1319', 'product_id' => '2513', 'publication_id' => '2947' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26559844" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'product' => array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human GAPDH promoter (TATA-box) primer pair' $meta_title = 'Human GAPDH promoter (TATA-box) primer pair' $product = array( 'Product' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ) ), 'Group' => array( 'Group' => array( 'id' => '134', 'name' => 'C17011001', 'product_id' => '2513', 'modified' => '2016-02-19 21:39:19', 'created' => '2016-02-19 21:39:19' ), 'Master' => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011001-50', 'old_catalog_number' => 'pp-1001-050', 'sf_code' => 'C17011001-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2020-05-27 14:42:11', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) ) $pro = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1001-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2514', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011001-500', 'old_catalog_number' => 'pp-1001-500', 'sf_code' => 'C17011001-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-tata-box-primer-pair-500ul', 'meta_title' => 'Human GAPDH promoter (TATA-box) primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter (TATA-box) primer pair', 'modified' => '2016-02-19 21:39:31', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '145', 'product_id' => '2514', 'group_id' => '134' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '234', 'name' => 'Datasheet Human GAPDH pp1001', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_pp1001.pdf', 'slug' => 'datasheet-human-gapdh-pp1001', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '602', 'product_id' => '2513', 'document_id' => '234' ) ) $publication = array( 'id' => '2947', 'name' => 'Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene', 'authors' => 'Henderson HH et al.', 'description' => '<p>Regulation of gene transcription in varicella-zoster virus (VZV), a ubiquitous human neurotropic alphaherpesvirus, requires coordinated binding of multiple host and virus proteins onto specific regions of the virus genome. Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( 'id' => '1319', 'product_id' => '2513', 'publication_id' => '2947' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26559844" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.