Infinium Mouse Methylation Array Service

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Description
End-to-end array

Bisulfite conversion

Whole genome amplification

Array hybridization

Single base extension

Array scanner

Analysis	Features
Standard	Standard files provided: Sample annotation Variable annotation Scanner output (IDAT files)
Differential methylation analysis	Identification of differentially methylated CpGs between sample groups. Files provided: Report with summary of differential methylation analysis and plots File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea) File containing differential methylated regions (DMRs)
Gene ontology terms analysis	Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.
Pathway analysis	Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.

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			'description' => '<p>Infinium MethylationEPIC＆Hydroxymethylation oxEPIC アレイは、選択的な酸化、バイサルファイト処理とIllumina&reg;技術に基づくゲノムワイドのDNAメチル化解析技術です。これは、一塩基分解能を有するゲノム全体にわたる850,000を超えるヒトCpG位置のメチル化レベル(5mC)と総メチル化（5mC + 5hmC）レベルの定量的な検出を可能にします。</p>
<p>バイサルファイト変換で、2種の5-メチルシトシンと5-ヒドロキシメチルシトシンのが判別なくシトシンとして読み取られ、DNAサンプルの総メチル化（5mC + 5hmC）レベルのマップが検出されます。 最初に酸化ステップを行うことで、5-ヒドロキシメチルシトシンは5-ホルミルシトシンに変換され、その後、バイサルファイト処理中にウラシルに変換されます。 その結果、5メチルシトシンのみがシトシンとして読み取られ、DNAサンプルのメチル化（5mC）レベルのマップが検出されます。 この2つの方法を組み合わせると、DNAサンプルの5-ヒドロキシメチルシトシン（5hmC）の状態を調べることが可能です。</p>
<h2>ゲノムワイドの包括的なカバレッジに最適な優れた方法</h2>
<ul class="square">
<li>所要時間の短縮、高いコストパフォーマンス</li>
<li>単一ヌクレオチド分解能で850,000を超えるヒトCpG位置</li>
<li><span style="font-family: inherit;">CpG、非CpG、およびCHH部位のメチル化部位の定量分析</span></li>
<li><span style="font-family: inherit;">5mCと5hmCの判別</span></li>
<li><span style="font-family: inherit; text-align: justify;">Differentially methylated とhydroxymethylated site解析</span></li>
<li><span style="font-family: inherit; text-align: justify;">エンドツーエンドの酸化変換、バイサルファイト変換、アレイハイブリダイゼーション、解析を含む包括的なサービス</span></li>
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<li style="font-weight: 400;"><span style="font-weight: 400;">Array hybridization</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Single base extension &nbsp;</span></li>
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<li>Sample annotation</li>
<li>Variable annotation</li>
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<p><strong>Differential methylation analysis</strong></p>
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<td style="width: 70%;">
<p>Identification of differentially methylated CpGs between sample groups.</p>
<p><em>Files provided:</em></p>
<ul>
<li>Report with summary of differential methylation analysis and plots</li>
<li>File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)</li>
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</td>
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<td style="vertical-align: top;">
<p><strong>Pathway analysis</strong></p>
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<p>バイサルファイト変換で、2種の5-メチルシトシンと5-ヒドロキシメチルシトシンのが判別なくシトシンとして読み取られ、DNAサンプルの総メチル化（5mC + 5hmC）レベルのマップが検出されます。 最初に酸化ステップを行うことで、5-ヒドロキシメチルシトシンは5-ホルミルシトシンに変換され、その後、バイサルファイト処理中にウラシルに変換されます。 その結果、5メチルシトシンのみがシトシンとして読み取られ、DNAサンプルのメチル化（5mC）レベルのマップが検出されます。 この2つの方法を組み合わせると、DNAサンプルの5-ヒドロキシメチルシトシン（5hmC）の状態を調べることが可能です。</p>
<h2>ゲノムワイドの包括的なカバレッジに最適な優れた方法</h2>
<ul class="square">
<li>所要時間の短縮、高いコストパフォーマンス</li>
<li>単一ヌクレオチド分解能で850,000を超えるヒトCpG位置</li>
<li><span style="font-family: inherit;">CpG、非CpG、およびCHH部位のメチル化部位の定量分析</span></li>
<li><span style="font-family: inherit;">5mCと5hmCの判別</span></li>
<li><span style="font-family: inherit; text-align: justify;">Differentially methylated とhydroxymethylated site解析</span></li>
<li><span style="font-family: inherit; text-align: justify;">エンドツーエンドの酸化変換、バイサルファイト変換、アレイハイブリダイゼーション、解析を含む包括的なサービス</span></li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/jp/categories/dna-methylation-profiling-services">Reduced Representation、全ゲノム、およびターゲット解析に関して、詳しくはその他のDNAメチル化プロファイリングサービスをご覧ください。</a></p>
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</td>
<td>
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<li style="font-weight: 400;">Bisulfite conversion</li>
<li style="font-weight: 400;">Whole genome amplification</li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Array hybridization</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Single base extension &nbsp;</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Array scanner&nbsp;</span></li>
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</td>
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<tr>
<td style="vertical-align: top;">
<p><strong>Standard</strong></p>
</td>
<td style="width: 70%;">
<p><em>Standard files provided:</em></p>
<ul>
<li>Sample annotation</li>
<li>Variable annotation</li>
<li>Scanner output&nbsp;(IDAT files)</li>
</ul>
</td>
</tr>
<tr>
<td style="vertical-align: top; width=30%; text-align: left;">
<p><strong>Differential methylation analysis</strong></p>
</td>
<td style="width: 70%;">
<p>Identification of differentially methylated CpGs between sample groups.</p>
<p><em>Files provided:</em></p>
<ul>
<li>Report with summary of differential methylation analysis and plots</li>
<li>File containing the differentially methylated CpGs and breakdown of those positions in regional analysis (CpG islands, shelves, shores and open sea)</li>
<li>File containing differential methylated regions (DMRs)</li>
</ul>
</td>
</tr>
<tr>
<td style="vertical-align: top;">
<p><strong>Gene ontology terms analysis</strong></p>
</td>
<td style="width: 70%;">
<p>Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.</p>
</td>
</tr>
<tr>
<td style="vertical-align: top;">
<p><strong>Pathway analysis</strong></p>
</td>
<td style="width: 70%;">
<p>Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.</p>
</td>
</tr>
</tbody>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
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[main] - APP/webroot/index.php, line 118

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	'meta_keywords' => 'Infinium Mouse Methylation Array Service',
	'meta_description' => 'Methylation profiling microarray service. Assess 275,000 methylation sites quantitatively across the mouse genome at single-nucleotide resolution.',
	'meta_title' => 'Infinium Mouse Methylation Array Service - Methylation Profiling Microarray | Diagenode',
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			'antibody_id' => null,
			'name' => 'Infinium Mouse Methylation Array Service',
			'description' => '<p>Infinium MethylationEPIC＆Hydroxymethylation oxEPIC アレイは、選択的な酸化、バイサルファイト処理とIllumina&reg;技術に基づくゲノムワイドのDNAメチル化解析技術です。これは、一塩基分解能を有するゲノム全体にわたる850,000を超えるヒトCpG位置のメチル化レベル(5mC)と総メチル化（5mC + 5hmC）レベルの定量的な検出を可能にします。</p>
<p>バイサルファイト変換で、2種の5-メチルシトシンと5-ヒドロキシメチルシトシンのが判別なくシトシンとして読み取られ、DNAサンプルの総メチル化（5mC + 5hmC）レベルのマップが検出されます。 最初に酸化ステップを行うことで、5-ヒドロキシメチルシトシンは5-ホルミルシトシンに変換され、その後、バイサルファイト処理中にウラシルに変換されます。 その結果、5メチルシトシンのみがシトシンとして読み取られ、DNAサンプルのメチル化（5mC）レベルのマップが検出されます。 この2つの方法を組み合わせると、DNAサンプルの5-ヒドロキシメチルシトシン（5hmC）の状態を調べることが可能です。</p>
<h2>ゲノムワイドの包括的なカバレッジに最適な優れた方法</h2>
<ul class="square">
<li>所要時間の短縮、高いコストパフォーマンス</li>
<li>単一ヌクレオチド分解能で850,000を超えるヒトCpG位置</li>
<li><span style="font-family: inherit;">CpG、非CpG、およびCHH部位のメチル化部位の定量分析</span></li>
<li><span style="font-family: inherit;">5mCと5hmCの判別</span></li>
<li><span style="font-family: inherit; text-align: justify;">Differentially methylated とhydroxymethylated site解析</span></li>
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<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/jp/categories/dna-methylation-profiling-services">Reduced Representation、全ゲノム、およびターゲット解析に関して、詳しくはその他のDNAメチル化プロファイリングサービスをご覧ください。</a></p>
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<li style="font-weight: 400;"><span style="font-weight: 400;">Single base extension &nbsp;</span></li>
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<li>所要時間の短縮、高いコストパフォーマンス</li>
<li>単一ヌクレオチド分解能で850,000を超えるヒトCpG位置</li>
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<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/jp/categories/dna-methylation-profiling-services">Reduced Representation、全ゲノム、およびターゲット解析に関して、詳しくはその他のDNAメチル化プロファイリングサービスをご覧ください。</a></p>
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<li style="font-weight: 400;">Whole genome amplification</li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Array hybridization</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Single base extension &nbsp;</span></li>
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View::render() - CORE/Cake/View/View.php, line 473
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<h2>ゲノムワイドの包括的なカバレッジに最適な優れた方法</h2>
<ul class="square">
<li>所要時間の短縮、高いコストパフォーマンス</li>
<li>単一ヌクレオチド分解能で850,000を超えるヒトCpG位置</li>
<li><span style="font-family: inherit;">CpG、非CpG、およびCHH部位のメチル化部位の定量分析</span></li>
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<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/jp/categories/dna-methylation-profiling-services">Reduced Representation、全ゲノム、およびターゲット解析に関して、詳しくはその他のDNAメチル化プロファイリングサービスをご覧ください。</a></p>
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<li style="font-weight: 400;"><span style="font-weight: 400;">Single base extension &nbsp;</span></li>
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<h2>ゲノムワイドの包括的なカバレッジに最適な優れた方法</h2>
<ul class="square">
<li>所要時間の短縮、高いコストパフォーマンス</li>
<li>単一ヌクレオチド分解能で850,000を超えるヒトCpG位置</li>
<li><span style="font-family: inherit;">CpG、非CpG、およびCHH部位のメチル化部位の定量分析</span></li>
<li><span style="font-family: inherit;">5mCと5hmCの判別</span></li>
<li><span style="font-family: inherit; text-align: justify;">Differentially methylated とhydroxymethylated site解析</span></li>
<li><span style="font-family: inherit; text-align: justify;">エンドツーエンドの酸化変換、バイサルファイト変換、アレイハイブリダイゼーション、解析を含む包括的なサービス</span></li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/jp/categories/dna-methylation-profiling-services">Reduced Representation、全ゲノム、およびターゲット解析に関して、詳しくはその他のDNAメチル化プロファイリングサービスをご覧ください。</a></p>
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<li style="font-weight: 400;"><span style="font-weight: 400;">Array hybridization</span></li>
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