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Inosine Antibody (sample size)

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Format

Price

C15200251-10

10 µg

$115.00
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C15200251

50 µg/29 µl

Monoclonal antibody raised in mouse using Inosine (I) conjugated to BSA as immunogen.

Lot	001
Concentration	1.75 µg/µl
Species reactivity	Human, other (wide range): positive.
Type	Monoclonal
Purity	Protein A purified monoclonal antibody.
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	PBS containing 0.05% sodium azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP ^*	1 µg per IP	Fig 1, 2 ,3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 10 µg per IP.

Validation Data

A.

B.

Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine
IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 µg total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 µg was analyzed. IgG (2 µg/IP) was used as negative control.
Figure 1A The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody.
Figure 1B shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.
実験手法
RIP
RIP Read more

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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine</strong><br />IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 &micro;g total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 &micro;g was analyzed. IgG (2 &micro;g/IP) was used as negative control. <br /><strong>Figure 1A</strong> The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody. <br /><strong>Figure 1B</strong> shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.</p>
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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine</strong><br />IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 &micro;g total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 &micro;g was analyzed. IgG (2 &micro;g/IP) was used as negative control. <br /><strong>Figure 1A</strong> The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody. <br /><strong>Figure 1B</strong> shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.</p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 10 &micro;g per IP.</small></p>',
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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine</strong><br />IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 &micro;g total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 &micro;g was analyzed. IgG (2 &micro;g/IP) was used as negative control. <br /><strong>Figure 1A</strong> The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody. <br /><strong>Figure 1B</strong> shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.</p>
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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine</strong><br />IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 &micro;g total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 &micro;g was analyzed. IgG (2 &micro;g/IP) was used as negative control. <br /><strong>Figure 1A</strong> The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody. <br /><strong>Figure 1B</strong> shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.</p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine</strong><br />IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 &micro;g total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 &micro;g was analyzed. IgG (2 &micro;g/IP) was used as negative control. <br /><strong>Figure 1A</strong> The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody. <br /><strong>Figure 1B</strong> shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.</p>
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<p></p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/c15200251-fig1a-rip.png" width="650" /></center></div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against Inosine</strong><br />IP was performed with the Diagenode antibody against Inosine (cat. No. C15200251) on 40 &micro;g total RNA from human HeLa cells, spiked with an in vitro produced RNA molecule containing Inosine nucleotides as well as an unmodified control RNA (40 ng each). A titration of the antibody of 1, 2 and 5 &micro;g was analyzed. IgG (2 &micro;g/IP) was used as negative control. <br /><strong>Figure 1A</strong> The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. The peak at ~500 bp corresponds to the Inosine spike in, whereas the negative control spike in (expected size ~300 bp) is not captured by the antibody. <br /><strong>Figure 1B</strong> shows the gel image for the Inosine antibody (lane 1, 2 and 3), the IgG negative control (lane 4) and the input (lane 5). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA and of both spike in controls is indicated on the right.</p>
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Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Datasheet Inosine Antibody C15200251 DATASHEET	Download

Inosine Antibody SDS US en	Download
Inosine Antibody SDS GB en	Download
Inosine Antibody SDS BE fr	Download
Inosine Antibody SDS FR fr	Download
Inosine Antibody SDS ES es	Download
Inosine Antibody SDS DE de	Download
Inosine Antibody SDS JP ja	Download
Inosine Antibody SDS BE nl	Download

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