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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>JARID1C (Jumonji, AT Rich Interactive Domain 1C)</strong>, using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>JARID1C (Jumonji, AT Rich Interactive Domain 1C)</strong>, using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-chip.jpg" alt="JARID1C Antibody ChIP Grade" caption="false" width="447" height="339" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-chipseq-a.jpg" alt="JARID1C Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-chipseq-b.jpg" alt="JARID1C Antibody for ChIP-seq" caption="false" width="700" height="164" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-chipseq-c.jpg" alt="JARID1C Antibody for ChIP-seq assay" caption="false" width="700" height="178" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-chipseq-d.jpg" alt="JARID1C Antibody validated in ChIP-seq" caption="false" width="700" height="130" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-wb.jpg" alt="JARID1C Antibody validated in Western blot" width="223" height="294" caption="false" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-ip.jpg" alt="JARID1C Antibody validated in Immunoprecipitation " width="145" height="255" caption="false" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-wb.jpg" alt="JARID1C Antibody validated in Western blot" width="223" height="294" caption="false" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410338-ip.jpg" alt="JARID1C Antibody validated in Immunoprecipitation " width="145" height="255" caption="false" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against JARID1C (Cat. No. C15410338) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the active EIF4A2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JARID1C</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 5 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ALDOA gene and the EIF4A2 positive control gene (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against JARID1C</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against JARID1C (Cat. No. C15410338) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against JARID1C</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against JARID1C (Cat. No. C15410338, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JARID1C protein was detected by western blot with the JARID1C antibody diluted 1:200.</small></p>
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