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KAT2B polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15410335

100 μl

$460.00
Bulk order

Other format

C15410335-20

20 μl

Polyclonal antibody raised in rabbit against human KAT2B (Lysine Acetyltransferase 2B), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

Lot	A301-666A2
Concentration	100 µl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Storage Conditions	Store at 4°C
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	2 μg/ChIP	Fig 1, 2
Western Blotting	1:1,000	Fig 3

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B
ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B
ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 μg of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).

Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target Description
KAT2B (UniProtKB/Swiss-Prot entry Q92831) functions as a histone acetyltransferase, thereby promoting transcriptional activation. KAT2B binds with CBP and p300 where it competes with the adenoviral oncoprotein E1A for binding sites and as such counteracts the mitogenic activity of E1A. KAT2B also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-wb.png" alt="KAT2B Antibody validated in Western Blot" width="180" height="246" caption="false" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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		'slug' => 'kat2b-polyclonal-antibody-100-ul',
		'meta_title' => 'KAT2B polyclonal antibody | Diagenode',
		'meta_keywords' => 'KAT2B (Lysine Acetyltransferase 2B) polyclonal antibody,Western blot',
		'meta_description' => 'KAT2B (Lysine Acetyltransferase 2B) polyclonal antibody from diagenode raised in rabbit against human has been validated for use in Western blot',
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		'id' => '497',
		'name' => 'KAT2B polyclonal antibody',
		'description' => 'KAT2B (UniProtKB/Swiss-Prot entry Q92831) functions as a histone acetyltransferase, thereby promoting transcriptional activation. KAT2B binds with CBP and p300 where it competes with the adenoviral oncoprotein E1A for binding sites and as such counteracts the mitogenic activity of E1A. KAT2B also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A301-666A2',
		'concentration' => '100 µl',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified ',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td><span>Western Blotting</span></td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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		'Master' => array(
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			'name' => 'KAT2B Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>KAT2B (Lysine Acetyltransferase 2B)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chip.png" alt="ChIPKAT2B Antibody ChIP Grade" caption="false" width="288" height="220" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-a.jpg" alt="KAT2B Antibody ChIP-seq Grade" caption="false" height="34" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-b.jpg" alt="ChIP-seqKAT2B Antibody for ChIP-seq" caption="false" height="67" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-c.jpg" alt="KAT2B Antibody for ChIP-seq assay" caption="false" height="52" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-d.jpg" alt="ChIP-seqKAT2B Antibody validated in ChIP-seq" caption="false" height="60" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-wb.png" alt="KAT2B Antibody validated in Western Blot" width="180" height="246" caption="false" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'KAT2B polyclonal antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human KAT2B (Lysine Acetyltransferase 2B), using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chip.png" alt="ChIPKAT2B Antibody ChIP Grade" caption="false" width="288" height="220" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td><span>Western Blotting</span></td>
<td>1:1,000</td>
<td>Fig 3</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-a.jpg" alt="KAT2B Antibody ChIP-seq Grade" caption="false" height="34" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-b.jpg" alt="ChIP-seqKAT2B Antibody for ChIP-seq" caption="false" height="67" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-c.jpg" alt="KAT2B Antibody for ChIP-seq assay" caption="false" height="52" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-chipseq-d.jpg" alt="ChIP-seqKAT2B Antibody validated in ChIP-seq" caption="false" height="60" /></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410335-wb.png" alt="KAT2B Antibody validated in Western Blot" width="180" height="246" caption="false" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B</strong><br /> ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 &mu;g of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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$application = array(
	'id' => '42',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'ChIP-seq (ab)',
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	'created' => '2015-10-20 11:44:45',
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	'position' => '10',
	'parent_id' => '40',
	'name' => 'ChIP-seq (ab)',
	'description' => '',
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	'slug' => 'chip-seq-antibodies',
	'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
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	'created' => '2015-10-20 11:44:45',
	'locale' => 'jpn'
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$description = ''
$name = 'ChIP-seq (ab)'
$document = array(
	'id' => '38',
	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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	'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
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	'meta_description' => '',
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	'countries' => 'BE',
	'modified' => '2024-01-17 18:44:16',
	'created' => '2024-01-17 18:44:16',
	'ProductsSafetySheet' => array(
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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KAT2B polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human KAT2B (Lysine Acetyltransferase 2B), using a s...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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KAT2B polyclonal antibody

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