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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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'description' => '<p><strong>other names:</strong> LMNA, LMN1, CMT2B1, LGMD1B, CDCD1, FPLD2, Lamin, CMD1A, LMNL1, CDDC, EMD2, FPLD, HGPS, LDP1, LMNC, MADA, PRO1, FPL, IDC, LFP</p><p>Polyclonal antibody raised in rabbit against LMNA (Lamin A/C) using a recombinant protein.</p>',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from NIH3T3 using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The antibody was used at different dilutions. The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />Western blot was performed on protein extracts from HeLa, 293T, NIH-3T3, Rat1, BHK-21 and CV-1 cells (lanes 1-6, respectively) using the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The marker is shown on the left, the position of Lamin A and Lamin C is indicated on the right.</small></p>
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<p><small><strong>Figure 3. IP using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />IP was performed on whole cell extracts (300 µg) from HeLa cells ectopically expressing Flag-tagged lamin A using 5 µg of the Diagenode antibody against Lamin A/C (Cat. No. C15200243). The immunoprecipitated proteins were subsequently analysed by Western blot with the Lamin A/C antibody as described above. Lane 3 shows the result of the IP, the input (15 µg) is shown in lane 1, lane 2 shows the cell lysate after the IP.</small></p>
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<p><small><strong>Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against Lamin A/C</strong><br />HeLa cells ectopically expressing Flag-tagged lamin A were fixed with 3.7% formaldehyde, permeabilized in 0,5% Triton X-100 and blocked in TBS containing 2% BSA. The cells were stained with the Lamin A/C antibody diluted 1:1,000 for 2 hours at RT, followed by incubation with an anti mouse secondary antibody coupled to AF596 for 1 h at RT (middle). Nuclei were counter-stained with Hoechst 33342 (left). A merge of the two stainings is shown on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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