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LSD1 polyclonal antibody (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15410067-10

10 µl

$115.00
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Other format

C15410067

50 µg

Alternative names: BHC110, AOF2, EC1, KDM1

Polyclonal antibody raised in rabbit against human LSD1 (Lysine-specific demethylase 1), using a KLH-conjugated synthetic peptide from the inner part of the protein.

Lot	A57-00234P
Concentration	2.2 µg/µl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP /ChIP-seq ^*	1 μg/ChIP	Fig 1, 2
ELISA	1:1,000 - 1:10,000	Fig 3
Western Blotting	1:4,000	Fig 4
Immunofluorescence	1:200	Fig 5

^* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1
ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the “iDeal ChIP-seq” kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1
ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 μg of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.

Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1
Western blot was performed using nuclear extracts from HeLa cells (40 μg) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.

Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1
Whole cell extracts (40 μg) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1
HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Target Description
LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated ‘Lys-4’ of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating ‘Lys-9’ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology

実験手法

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

ELISA
Enzyme-linked immunosorbent assay. Read more

ChIP-seq (ab)
Read more

ChIP-qPCR (ab)
Read more

siRNA Knockdown
Epigenetic antibodies you can trust! Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level o... Read more

出版物

How to properly cite this product in your work

Diagenode strongly recommends using this: LSD1 polyclonal antibody (sample size) (Diagenode Cat# C15410067-10 Lot# A57-00234P). Click here to copy to clipboard.

Using our products in your publication? Let us know!

Interactome Networks of FOSL1 and FOSL2 in Human Th17 Cells.
Shetty Ankitha et al.
Dysregulated function of Th17 cells has implications in immunodeficiencies and autoimmune disorders. Th17 cell differentiation is orchestrated by a complex network of transcription factors, including several members of the activator protein (AP-1) family. Among the latter, FOSL1 and FOSL2 modulate the effector funct...

HOTAIR lncRNA promotes epithelial-mesenchymal transition byredistributing LSD1 at regulatory chromatin regions.
Jarroux, J. et al.
Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold for PRC2 and thus promoting repressive hi...

LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.
Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC
We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimeth...

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<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
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<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<th>References</th>
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<td>ChIP /ChIP-seq <sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:1,000 - 1:10,000</td>
<td>Fig 3</td>
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<tr>
<td>Western Blotting</td>
<td>1:4,000</td>
<td>Fig 4</td>
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			'antibody_id' => '251',
			'name' => 'LSD1 Antibody',
			'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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</div>
<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
</div>',
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			'info2' => '<p>LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated &lsquo;Lys-4&rsquo; of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating &lsquo;Lys-9&rsquo; of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology</p>',
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			'meta_description' => 'LSD1 (Lysine-specific demethylase 1) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA, WB and IF. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: BHC110, AOF2, EC1, KDM1',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
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			'description' => '<div class="row">
<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
</div>
<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'meta_title' => 'Sample-size Antibodies | Diagenode',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => 'ChIP-seq grade antibodies',
			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'name' => 'Histone modifying enzymes',
			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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			'description' => '<p>Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold for PRC2 and thus promoting repressive histone H3K27 methylation. In addition to PRC2, HOTAIR interacts with the LSD1 lysine demethylase, an epigenetic regulator of cell fate during development and differentiation, but little is known about the role of LSD1 in HOTAIR function during EMT. Here, we show that HOTAIR requires its LSD1-interacting domain, but not its PRC2-interacting domain, to promote the migration of epithelial cells. This activity is suppressed by LSD1 overexpression. LSD1-HOTAIR interactions induce partial reprogramming of the epithelial transcriptome altering LSD1 distribution at promoter and enhancer regions. Thus, we uncover an unexpected role of HOTAIR in EMT as an LSD1 decommissioning factor, counteracting its activity in the control of epithelial identity.</p>',
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<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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	'antibody_id' => '251',
	'name' => 'LSD1 Antibody',
	'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
	'label1' => 'Validation Data',
	'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
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	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '380',
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	'created' => '2015-06-29 14:08:20'
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	'name' => 'siRNA Knockdown',
	'description' => '<div class="row">
<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
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<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
</div>
<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>'
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	'name' => 'LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.',
	'authors' => 'Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC',
	'description' => 'We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.',
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	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21602794',
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View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
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<tr>
<td>ChIP /ChIP-seq <sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:1,000 - 1:10,000</td>
<td>Fig 3</td>
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<tr>
<td>Western Blotting</td>
<td>1:4,000</td>
<td>Fig 4</td>
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<td>Immunofluorescence</td>
<td>1:200</td>
<td>Fig 5</td>
</tr>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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		'antibody_id' => '251',
		'name' => 'LSD1 polyclonal antibody (sample size)',
		'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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		'meta_title' => 'LSD1 Polyclonal Antibody (sample size) | Diagenode',
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		'meta_description' => 'LSD1 (Lysine-specific demethylase 1) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA, WB and IF. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: BHC110, AOF2, EC1, KDM1',
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		'description' => 'LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated ‘Lys-4’ of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating ‘Lys-9’ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A57-00234P',
		'concentration' => '2.2 &micro;g/&micro;l',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP /ChIP-seq <sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:1,000 - 1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:4,000</td>
<td>Fig 4</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:200</td>
<td>Fig 5</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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			'product_id' => '2228',
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			'antibody_id' => '251',
			'name' => 'LSD1 Antibody',
			'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
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<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
</div>
<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'description' => '<p>Dysregulated function of Th17 cells has implications in immunodeficiencies and autoimmune disorders. Th17 cell differentiation is orchestrated by a complex network of transcription factors, including several members of the activator protein (AP-1) family. Among the latter, FOSL1 and FOSL2 modulate the effector functions of Th17 cells. However, the molecular mechanisms underlying these effects are unclear, owing to the poorly characterized protein interaction networks of FOSL factors. Here, we establish the first interactomes of FOSL1 and FOSL2 in human Th17 cells, using affinity purification-mass spectrometry analysis. In addition to the known JUN proteins, we identified several novel binding partners of FOSL1 and FOSL2. Gene ontology analysis found a significant fraction of these interactors to be associated with RNA-binding activity, which suggests new mechanistic links. Intriguingly, 29 proteins were found to share interactions with FOSL1 and FOSL2, and these included key regulators of Th17 fate. We further validated the binding partners identified in this study by using parallel reaction monitoring targeted mass spectrometry and other methods. Our study provides key insights into the interaction-based signaling mechanisms of FOSL proteins that potentially govern Th17 cell differentiation and associated pathologies.</p>',
			'date' => '2021-09-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34604665',
			'doi' => '10.1021/acsomega.1c03681',
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			'description' => '<p>Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold for PRC2 and thus promoting repressive histone H3K27 methylation. In addition to PRC2, HOTAIR interacts with the LSD1 lysine demethylase, an epigenetic regulator of cell fate during development and differentiation, but little is known about the role of LSD1 in HOTAIR function during EMT. Here, we show that HOTAIR requires its LSD1-interacting domain, but not its PRC2-interacting domain, to promote the migration of epithelial cells. This activity is suppressed by LSD1 overexpression. LSD1-HOTAIR interactions induce partial reprogramming of the epithelial transcriptome altering LSD1 distribution at promoter and enhancer regions. Thus, we uncover an unexpected role of HOTAIR in EMT as an LSD1 decommissioning factor, counteracting its activity in the control of epithelial identity.</p>',
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			'authors' => 'Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC',
			'description' => 'We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.',
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<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
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<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
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<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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	'name' => 'LSD1 Antibody',
	'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
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	'name' => 'siRNA Knockdown',
	'description' => '<div class="row">
<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
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<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
</div>
<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>'
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	'description' => 'We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.',
	'date' => '2011-06-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21602794',
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	'modified' => '2015-07-24 15:38:58',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<div class="small-4 columns">
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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			'name' => 'ChIP-grade antibodies',
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
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			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'date' => '2021-09-01',
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			'doi' => '10.1021/acsomega.1c03681',
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			'description' => '<p>Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold for PRC2 and thus promoting repressive histone H3K27 methylation. In addition to PRC2, HOTAIR interacts with the LSD1 lysine demethylase, an epigenetic regulator of cell fate during development and differentiation, but little is known about the role of LSD1 in HOTAIR function during EMT. Here, we show that HOTAIR requires its LSD1-interacting domain, but not its PRC2-interacting domain, to promote the migration of epithelial cells. This activity is suppressed by LSD1 overexpression. LSD1-HOTAIR interactions induce partial reprogramming of the epithelial transcriptome altering LSD1 distribution at promoter and enhancer regions. Thus, we uncover an unexpected role of HOTAIR in EMT as an LSD1 decommissioning factor, counteracting its activity in the control of epithelial identity.</p>',
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			'authors' => 'Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC',
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		'name' => 'LSD1 Antibody',
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<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<div class="small-4 columns">
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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	'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
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<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
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<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
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<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<h3>Epigenetic antibodies you can trust!</h3>
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<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
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<h3>Epigenetic antibodies you can trust!</h3>
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<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
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</div>
</div>
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<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>'
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated &lsquo;Lys-4&rsquo; of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating &lsquo;Lys-9&rsquo; of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology</p>',
		'label3' => '',
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		'format' => '10 µl',
		'catalog_number' => 'C15410067-10',
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		'sf_code' => 'C15410067-D001-000582',
		'type' => 'RFR',
		'search_order' => '03-Antibody',
		'price_EUR' => '105',
		'price_USD' => '115',
		'price_GBP' => '100',
		'price_JPY' => '16450',
		'price_CNY' => '0',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
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		'slug' => 'lsd1-polyclonal-antibody-classic-10-ug',
		'meta_title' => 'LSD1 Polyclonal Antibody (sample size) | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'LSD1 (Lysine-specific demethylase 1) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA, WB and IF. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: BHC110, AOF2, EC1, KDM1',
		'modified' => '2024-01-16 14:47:12',
		'created' => '2020-01-27 12:15:44',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '251',
		'name' => 'LSD1 polyclonal antibody',
		'description' => 'LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated ‘Lys-4’ of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating ‘Lys-9’ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A57-00234P',
		'concentration' => '2.2 &micro;g/&micro;l',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP /ChIP-seq <sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:1,000 - 1:10,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:4,000</td>
<td>Fig 4</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:200</td>
<td>Fig 5</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2020-08-04 10:29:48',
		'created' => '0000-00-00 00:00:00',
		'select_label' => '251 - LSD1 polyclonal antibody (A57-00234P - 2.2 &micro;g/&micro;l - Human - Affinity purified - Rabbit)'
	),
	'Slave' => array(),
	'Group' => array(
		'Group' => array(
			'id' => '270',
			'name' => 'C15410067',
			'product_id' => '2228',
			'modified' => '2020-01-27 12:16:17',
			'created' => '2020-01-27 12:16:17'
		),
		'Master' => array(
			'id' => '2228',
			'antibody_id' => '251',
			'name' => 'LSD1 Antibody',
			'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p>LSD1 (lysine specific demethylase 1, UniProt/Swiss-Prot entry O60341) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated &lsquo;Lys-4&rsquo; of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating &lsquo;Lys-9&rsquo; of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore mediates different processes such as embryonic development, cell differentiation and proliferation, stem and cancer cell biology</p>',
			'label3' => '',
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			'format' => '50 µg',
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			'price_EUR' => '380',
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			'price_JPY' => '59525',
			'price_CNY' => '',
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			'country' => 'ALL',
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			'slug' => 'lsd1-polyclonal-antibody-classic-50-ug',
			'meta_title' => 'LSD1 Antibody - ChIP-seq Grade (C15410067) | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'LSD1 (Lysine-specific demethylase 1) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA, WB and IF. Specificity confirmed by siRNA assay. Batch-specific data available on the website. Alternative names: BHC110, AOF2, EC1, KDM1',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
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			'description' => '<div class="row">
<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
</div>
<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'name' => 'ChIP-seq grade antibodies',
			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'name' => 'Datasheet LSD1 C15410067',
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			'authors' => 'Shetty Ankitha et al.',
			'description' => '<p>Dysregulated function of Th17 cells has implications in immunodeficiencies and autoimmune disorders. Th17 cell differentiation is orchestrated by a complex network of transcription factors, including several members of the activator protein (AP-1) family. Among the latter, FOSL1 and FOSL2 modulate the effector functions of Th17 cells. However, the molecular mechanisms underlying these effects are unclear, owing to the poorly characterized protein interaction networks of FOSL factors. Here, we establish the first interactomes of FOSL1 and FOSL2 in human Th17 cells, using affinity purification-mass spectrometry analysis. In addition to the known JUN proteins, we identified several novel binding partners of FOSL1 and FOSL2. Gene ontology analysis found a significant fraction of these interactors to be associated with RNA-binding activity, which suggests new mechanistic links. Intriguingly, 29 proteins were found to share interactions with FOSL1 and FOSL2, and these included key regulators of Th17 fate. We further validated the binding partners identified in this study by using parallel reaction monitoring targeted mass spectrometry and other methods. Our study provides key insights into the interaction-based signaling mechanisms of FOSL proteins that potentially govern Th17 cell differentiation and associated pathologies.</p>',
			'date' => '2021-09-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34604665',
			'doi' => '10.1021/acsomega.1c03681',
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			'name' => 'HOTAIR lncRNA promotes epithelial-mesenchymal transition byredistributing LSD1 at regulatory chromatin regions.',
			'authors' => 'Jarroux, J. et al.',
			'description' => '<p>Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold for PRC2 and thus promoting repressive histone H3K27 methylation. In addition to PRC2, HOTAIR interacts with the LSD1 lysine demethylase, an epigenetic regulator of cell fate during development and differentiation, but little is known about the role of LSD1 in HOTAIR function during EMT. Here, we show that HOTAIR requires its LSD1-interacting domain, but not its PRC2-interacting domain, to promote the migration of epithelial cells. This activity is suppressed by LSD1 overexpression. LSD1-HOTAIR interactions induce partial reprogramming of the epithelial transcriptome altering LSD1 distribution at promoter and enhancer regions. Thus, we uncover an unexpected role of HOTAIR in EMT as an LSD1 decommissioning factor, counteracting its activity in the control of epithelial identity.</p>',
			'date' => '2021-05-01',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33960111',
			'doi' => '10.15252/embr.202050193',
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			'name' => 'LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.',
			'authors' => 'Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC',
			'description' => 'We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.',
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		'name' => 'LSD1 Antibody',
		'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
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<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
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	'description' => '<p><span>Alternative names: <strong>BHC110</strong>, <strong>AOF2</strong>, <strong>EC1</strong>, <strong>KDM1</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> LSD1 (Lysine-specific demethylase 1)</strong>, using a KLH-conjugated synthetic peptide from the inner part of the protein.</span></p>',
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<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIP.jpg" alt="LSD1 Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed with the Diagenode antibody against LSD1 (Cat. No. C15410067) on sheared chromatin from 4,000,000 K562 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055).. An antibody titration consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. QPCR was performed with primers for specific regions in the MYT1, RBM19, and TGFBR3 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_A.jpg" alt="LSD1 Antibody ChIP-seq Grade" caption="false" width="447" height="54" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_B.jpg" alt="LSD1 Antibody for ChIP-seq" caption="false" width="447" height="83" /></p>
<p><img src="http://www.diagenode.com//img/product/antibodies/C15410067_ChIPSeq_C.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="70" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_D.jpg" alt="LSD1 Antibody for ChIP-seq assay" caption="false" width="447" height="76" /></p>
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ChIPSeq_E.jpg" alt="LSD1 Antibody validated in ChIP-seq" caption="false" width="447" height="86" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against LSD1</strong><br /> ChIP was performed on sheared chromatin from 4,000,000 K562 cells using 1 &mu;g of the Diagenode antibody against LSD1 (cat. No. C15410067) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B) and in three regions surrounding the MYT1, RBM19 and TGFBR3 positive control genes, respectively (figure 2C, D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_ELISA.jpg" alt="LSD1 Antibody ELISA validation" caption="false" width="288" height="217" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br /> To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against LSD1 (Cat. No. C15410067) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB.jpg" alt="LSD1 Antibody validated in Western Blot" caption="false" width="200" height="290" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Western blot was performed using nuclear extracts from HeLa cells (40 &mu;g) and the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:4,000 in TBS- Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_WB_2.png" alt="LSD1 Antibody validated in Western Blot" caption="false" width="288" height="373" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 5. Western blot analysis using the Diagenode antibody directed against LSD1</strong><br /> Whole cell extracts (40 &mu;g) from HeLa cells transfected with LSD1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against LSD1 (Cat. No. C15410067) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="http://www.diagenode.com/img/product/antibodies/C15410067_IF.jpg" alt="LSD1 Antibody validated in Immunofluorescence" caption="false" width="367" height="90" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 6. Immunofluorescence using the Diagenode antibody directed against LSD1</strong><br /> HeLa cells were stained with the Diagenode antibody against LSD1 (Cat. No. C15410067) and with DAPI. Cells were fixed with 4% formaldehyde for 10&rsquo; and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the LSD1 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.</small></p>
</div>
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	'name' => 'siRNA Knockdown',
	'description' => '<div class="row">
<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
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<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<div class="small-10 columns">
<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<div class="small-2 columns">
<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>',
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<h3>Epigenetic antibodies you can trust!</h3>
<p>Antibody quality is essential for assay success. Diagenode offers antibodies that are actually validated and have been widely used and published by the scientific community. Now we are adding a new level of siRNA knockdown validation to assure the specificity of our non-histone antibodies.</p>
<p><strong>Short interfering RNA (siRNA)</strong> degrades target mRNA, followed by the knock-down of protein production. If the antibody that recognizes the protein of interest is specific, the Western blot of siRNA-treated cells will show a significant reduction of signal vs. untreated cells.</p>
<center><img src="https://www.diagenode.com/emailing/images/C15100144-wb.png" alt="" /></center>
<p class="text-center"><small>WB results obtained with the HDAC1 pAb (Cat. No. C15100144) <br />on siRNA transfected cells (lane 2) and on untransfected control cells (lane 1).</small></p>
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<p><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></p>
</div>
</div>
<div class="spaced"></div>
<p style="text-align: left;"><span style="font-weight: 400;">The below list shows our first siRNA validated antibodies. More results - coming soon</span>.</p>'
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	'id' => '626',
	'name' => 'LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells.',
	'authors' => 'Adamo A, Sesé B, Boue S, Castaño J, Paramonov I, Barrero MJ, Izpisua Belmonte JC',
	'description' => 'We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and AOF2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.',
	'date' => '2011-06-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/21602794',
	'doi' => '',
	'modified' => '2015-07-24 15:38:58',
	'created' => '2015-07-24 15:38:58',
	'ProductsPublication' => array(
		'id' => '4236',
		'product_id' => '3052',
		'publication_id' => '626'
	)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/21602794" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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