Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'product' => array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
)
$language = 'jp'
$meta_keywords = 'N6-methyladenosine (m6A),monoclonal antibody, sample size'
$meta_description = 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.'
$meta_title = 'N6-methyladenosine (m6A) monoclonal antibody (sample size)'
$product = array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '215',
'name' => 'C15200082',
'product_id' => '2788',
'modified' => '2017-06-22 11:52:13',
'created' => '2017-06-22 11:52:13'
),
'Master' => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '28',
'position' => '10',
'parent_id' => '40',
'name' => 'DB',
'description' => '<p>Dot blotting</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'dot-blotting',
'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 14:40:49',
'created' => '2015-07-08 13:45:05',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '109',
'position' => '40',
'parent_id' => '4',
'name' => 'RNA modifications',
'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'rna-modifications-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody',
'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies',
'meta_title' => 'RNA modifications antibodies | Diagenode.com',
'modified' => '2020-09-01 11:43:28',
'created' => '2016-02-15 10:58:08',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '250',
'name' => 'product/antibodies/antibody.png',
'alt' => 'Mouse IgG',
'modified' => '2020-11-27 07:00:09',
'created' => '2015-07-17 10:12:18',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4848',
'name' => 'Single-cell m6A mapping in vivo using picoMeRIP-seq',
'authors' => 'Li Y. et al.',
'description' => '<p>Current N-methyladenosine (mA) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale mA RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying mA in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark mA mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.</p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37349523',
'doi' => '10.1038/s41587-023-01831-7',
'modified' => '2023-08-01 14:21:03',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1406',
'name' => 'N6 methyladenosine(m6A) Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1408',
'name' => 'N6 methyladenosine(m6A) Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1403',
'name' => 'N6 methyladenosine(m6A) Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1407',
'name' => 'N6 methyladenosine(m6A) Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1402',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1401',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1405',
'name' => 'N6 methyladenosine(m6A) Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
)
$pro = array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) Antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) Antibody (C15200082-10) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'ProductsGroup' => array(
'id' => '240',
'product_id' => '2901',
'group_id' => '215'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
'id' => '4675',
'product_id' => '2901',
'application_id' => '36'
)
)
$slugs = array(
(int) 0 => 'rip'
)
$applications = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'locale' => 'jpn'
)
$description = '<p>RIP</p>'
$name = 'RIP'
$document = array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
'id' => '2380',
'product_id' => '2901',
'document_id' => '885'
)
)
$sds = array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
'id' => '2462',
'product_id' => '2901',
'safety_sheet_id' => '1404'
)
)
$publication = array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6071',
'product_id' => '2901',
'publication_id' => '4406'
)
)
$externalLink = ' <a href="https://doi.org/10.1101%2F2022.03.02.482749" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'product' => array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
)
$language = 'jp'
$meta_keywords = 'N6-methyladenosine (m6A),monoclonal antibody, sample size'
$meta_description = 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.'
$meta_title = 'N6-methyladenosine (m6A) monoclonal antibody (sample size)'
$product = array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '215',
'name' => 'C15200082',
'product_id' => '2788',
'modified' => '2017-06-22 11:52:13',
'created' => '2017-06-22 11:52:13'
),
'Master' => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '28',
'position' => '10',
'parent_id' => '40',
'name' => 'DB',
'description' => '<p>Dot blotting</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'dot-blotting',
'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 14:40:49',
'created' => '2015-07-08 13:45:05',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '109',
'position' => '40',
'parent_id' => '4',
'name' => 'RNA modifications',
'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'rna-modifications-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody',
'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies',
'meta_title' => 'RNA modifications antibodies | Diagenode.com',
'modified' => '2020-09-01 11:43:28',
'created' => '2016-02-15 10:58:08',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '250',
'name' => 'product/antibodies/antibody.png',
'alt' => 'Mouse IgG',
'modified' => '2020-11-27 07:00:09',
'created' => '2015-07-17 10:12:18',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4848',
'name' => 'Single-cell m6A mapping in vivo using picoMeRIP-seq',
'authors' => 'Li Y. et al.',
'description' => '<p>Current N-methyladenosine (mA) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale mA RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying mA in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark mA mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.</p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37349523',
'doi' => '10.1038/s41587-023-01831-7',
'modified' => '2023-08-01 14:21:03',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1406',
'name' => 'N6 methyladenosine(m6A) Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1408',
'name' => 'N6 methyladenosine(m6A) Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1403',
'name' => 'N6 methyladenosine(m6A) Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1407',
'name' => 'N6 methyladenosine(m6A) Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1402',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1401',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1405',
'name' => 'N6 methyladenosine(m6A) Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
)
$pro = array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) Antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) Antibody (C15200082-10) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'ProductsGroup' => array(
'id' => '240',
'product_id' => '2901',
'group_id' => '215'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
'id' => '4675',
'product_id' => '2901',
'application_id' => '36'
)
)
$slugs = array(
(int) 0 => 'rip'
)
$applications = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'locale' => 'jpn'
)
$description = '<p>RIP</p>'
$name = 'RIP'
$document = array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
'id' => '2380',
'product_id' => '2901',
'document_id' => '885'
)
)
$sds = array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
'id' => '2462',
'product_id' => '2901',
'safety_sheet_id' => '1404'
)
)
$publication = array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6071',
'product_id' => '2901',
'publication_id' => '4406'
)
)
$externalLink = ' <a href="https://doi.org/10.1101%2F2022.03.02.482749" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'product' => array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
)
$language = 'jp'
$meta_keywords = 'N6-methyladenosine (m6A),monoclonal antibody, sample size'
$meta_description = 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.'
$meta_title = 'N6-methyladenosine (m6A) monoclonal antibody (sample size)'
$product = array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '215',
'name' => 'C15200082',
'product_id' => '2788',
'modified' => '2017-06-22 11:52:13',
'created' => '2017-06-22 11:52:13'
),
'Master' => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '28',
'position' => '10',
'parent_id' => '40',
'name' => 'DB',
'description' => '<p>Dot blotting</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'dot-blotting',
'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 14:40:49',
'created' => '2015-07-08 13:45:05',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '109',
'position' => '40',
'parent_id' => '4',
'name' => 'RNA modifications',
'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'rna-modifications-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody',
'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies',
'meta_title' => 'RNA modifications antibodies | Diagenode.com',
'modified' => '2020-09-01 11:43:28',
'created' => '2016-02-15 10:58:08',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '250',
'name' => 'product/antibodies/antibody.png',
'alt' => 'Mouse IgG',
'modified' => '2020-11-27 07:00:09',
'created' => '2015-07-17 10:12:18',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4848',
'name' => 'Single-cell m6A mapping in vivo using picoMeRIP-seq',
'authors' => 'Li Y. et al.',
'description' => '<p>Current N-methyladenosine (mA) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale mA RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying mA in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark mA mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.</p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37349523',
'doi' => '10.1038/s41587-023-01831-7',
'modified' => '2023-08-01 14:21:03',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1406',
'name' => 'N6 methyladenosine(m6A) Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1408',
'name' => 'N6 methyladenosine(m6A) Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1403',
'name' => 'N6 methyladenosine(m6A) Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1407',
'name' => 'N6 methyladenosine(m6A) Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1402',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1401',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1405',
'name' => 'N6 methyladenosine(m6A) Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
)
$pro = array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) Antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) Antibody (C15200082-10) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'ProductsGroup' => array(
'id' => '240',
'product_id' => '2901',
'group_id' => '215'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
'id' => '4675',
'product_id' => '2901',
'application_id' => '36'
)
)
$slugs = array(
(int) 0 => 'rip'
)
$applications = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'locale' => 'jpn'
)
$description = '<p>RIP</p>'
$name = 'RIP'
$document = array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
'id' => '2380',
'product_id' => '2901',
'document_id' => '885'
)
)
$sds = array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
'id' => '2462',
'product_id' => '2901',
'safety_sheet_id' => '1404'
)
)
$publication = array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6071',
'product_id' => '2901',
'publication_id' => '4406'
)
)
$externalLink = ' <a href="https://doi.org/10.1101%2F2022.03.02.482749" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'product' => array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
)
$language = 'jp'
$meta_keywords = 'N6-methyladenosine (m6A),monoclonal antibody, sample size'
$meta_description = 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.'
$meta_title = 'N6-methyladenosine (m6A) monoclonal antibody (sample size)'
$product = array(
'Product' => array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) monoclonal antibody (sample size)',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'To demonstrate the specificity of the Diagenode monoclonal antibody againstN6-methyladenosine (m6A) , a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => '474',
'name' => 'm6A monoclonal antibody',
'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
'clonality' => '',
'isotype' => 'IgG1',
'lot' => '001',
'concentration' => '2 μg/μl',
'reactivity' => 'Human, mouse, other (wide range)',
'type' => 'Monoclonal',
'purity' => 'Protein A purified monoclonal antibody.',
'classification' => 'Classic',
'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td><span>RIP*</span></td>
<td><span>2 µg per IP</span></td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:500</td>
<td>Fig 3, 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.</small></p>',
'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
'storage_buffer' => 'PBS containing 0.05% azide.',
'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
'uniprot_acc' => '',
'slug' => 'm6a-monoclonal-antibody-c15200082',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-11-13 14:48:40',
'created' => '2016-02-17 16:04:27',
'select_label' => '474 - m6A monoclonal antibody (001 - 2 μg/μl - Human, mouse, other (wide range) - Protein A purified monoclonal antibody. - Mouse)'
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '215',
'name' => 'C15200082',
'product_id' => '2788',
'modified' => '2017-06-22 11:52:13',
'created' => '2017-06-22 11:52:13'
),
'Master' => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '28',
'position' => '10',
'parent_id' => '40',
'name' => 'DB',
'description' => '<p>Dot blotting</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'dot-blotting',
'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,',
'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications',
'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 14:40:49',
'created' => '2015-07-08 13:45:05',
'ProductsApplication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '102',
'position' => '1',
'parent_id' => '4',
'name' => 'Sample size antibodies',
'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation</li>
<li><strong>Classified</strong> based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => true,
'is_antibody' => true,
'slug' => 'sample-size-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode',
'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ',
'meta_title' => 'Sample-size Antibodies | Diagenode',
'modified' => '2019-07-03 10:57:05',
'created' => '2015-10-27 12:13:34',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '103',
'position' => '0',
'parent_id' => '4',
'name' => 'All antibodies',
'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'all-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
'modified' => '2019-07-03 10:55:44',
'created' => '2015-11-02 14:49:22',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 2 => array(
'id' => '109',
'position' => '40',
'parent_id' => '4',
'name' => 'RNA modifications',
'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'rna-modifications-antibodies',
'cookies_tag_id' => null,
'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody',
'meta_description' => 'Diagenode offers a wide range of RNA modifications antibodies',
'meta_title' => 'RNA modifications antibodies | Diagenode.com',
'modified' => '2020-09-01 11:43:28',
'created' => '2016-02-15 10:58:08',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '11',
'name' => 'Antibodies you can trust',
'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
'image_id' => null,
'type' => 'Poster',
'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
'slug' => 'antibodies-you-can-trust-poster',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-10-01 20:18:31',
'created' => '2015-07-03 16:05:15',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '250',
'name' => 'product/antibodies/antibody.png',
'alt' => 'Mouse IgG',
'modified' => '2020-11-27 07:00:09',
'created' => '2015-07-17 10:12:18',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4848',
'name' => 'Single-cell m6A mapping in vivo using picoMeRIP-seq',
'authors' => 'Li Y. et al.',
'description' => '<p>Current N-methyladenosine (mA) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale mA RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying mA in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark mA mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.</p>',
'date' => '2023-06-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37349523',
'doi' => '10.1038/s41587-023-01831-7',
'modified' => '2023-08-01 14:21:03',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '1406',
'name' => 'N6 methyladenosine(m6A) Antibody SDS GB en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-GB-en-GHS_2_0.pdf',
'countries' => 'GB',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1408',
'name' => 'N6 methyladenosine(m6A) Antibody SDS US en',
'language' => 'en',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-US-en-GHS_2_0.pdf',
'countries' => 'US',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1403',
'name' => 'N6 methyladenosine(m6A) Antibody SDS DE de',
'language' => 'de',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1407',
'name' => 'N6 methyladenosine(m6A) Antibody SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-JP-ja-GHS_3_0.pdf',
'countries' => 'JP',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1402',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1401',
'name' => 'N6 methyladenosine(m6A) Antibody SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-BE-fr-GHS_2_0.pdf',
'countries' => 'BE',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '1405',
'name' => 'N6 methyladenosine(m6A) Antibody SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-FR-fr-GHS_2_0.pdf',
'countries' => 'FR',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/m6a-monoclonal-antibody'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
)
$pro = array(
'id' => '2901',
'antibody_id' => '474',
'name' => 'N6-methyladenosine (m6A) Antibody (sample size)',
'description' => '<p><span>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</span></p>',
'label1' => 'Validation Data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 μg',
'catalog_number' => 'C15200082-10',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000582',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'm6a-monoclonal-antibody-10-ug',
'meta_title' => 'N6-methyladenosine (m6A) Antibody (C15200082-10) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody, sample size',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available',
'modified' => '2022-12-20 17:00:35',
'created' => '2017-06-22 11:51:33',
'ProductsGroup' => array(
'id' => '240',
'product_id' => '2901',
'group_id' => '215'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '2788',
'antibody_id' => '474',
'name' => ' N6-methyladenosine (m6A) Antibody',
'description' => '<p>Monoclonal antibody raised in mouse against <strong>N6-methyladenosine</strong> (<strong>m6A</strong>) conjugated to BSA.</p>',
'label1' => 'Validation Data',
'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP.jpg" alt="N6-methyladenosine (m6A) antibody for RIP" caption="false" width="278" height="296" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing 6mA nucleotides, using 2 µg of the Diagenode monoclonal m6A antibody (cat. nr. C15200082). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-RIP-2.jpg" alt="N6-methyladenosine (m6A) antibody for RNA immunoprecipitation" caption="false" width="278" height="156" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 2. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against m6A</strong> <br />Immunoprecipitation was performed on two radiolabelled synthetic RNA oligo’s, a 250 nt oligo containing m6A nucleotides and a 350 nt unmethylated control, using the Diagenode monoclonal m6A antibody (cat. nr. C15200082). The immunoprecipitated fraction is shown in lane 3; lane 2 shows the non bound fraction, whereas the input is shown in lane 1.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot.jpg" alt="N6-methyladenosine (m6A) antibody for dot blot" caption="false" width="278" height="185" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode monoclonal antibody against m6A (Cat. No. C15200082), a Dot Blot analysis was performed using an m6A containing, a non-methylated control and a non-methylated polyA control synthetic RNA oligonucleotide.</small></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200082-dotblot-2.jpg" alt="N6-methyladenosine (m6A) antibody validated in dot blot" caption="false" width="200" height="222" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 4. Dot blot analysis using the Diagenode monoclonal antibody directed against m6A</strong><br />Dot Blot analysis was performed using a synthetic DNA oligonucleotide containing different m6A modified bases and a negative control. 100 to 4 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted of 1:500 in TBS-T containing 10 % skimmed milk and 1% BSA. Figure 4 shows a high specificity of the antibody for the modified oligonucleotide.</small></p>
</div>
</div>',
'label2' => 'Target Description',
'info2' => '<p>N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development. Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.</p>',
'label3' => '',
'info3' => '',
'format' => '50 μg/ 25 μl',
'catalog_number' => 'C15200082',
'old_catalog_number' => '',
'sf_code' => 'C15200082-D001-000581',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '380',
'price_USD' => '380',
'price_GBP' => '340',
'price_JPY' => '59525',
'price_CNY' => '',
'price_AUD' => '950',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => 'October 1, 2018',
'slug' => 'm6a-monoclonal-antibody',
'meta_title' => 'N6-methyladenosine (m6A) antibody - RIP Grade (C15200082) | Diagenode',
'meta_keywords' => 'N6-methyladenosine (m6A),monoclonal antibody',
'meta_description' => 'N6-methyladenosine (m6A) Monoclonal Antibody validated in RIP and DB. Batch-specific data available on the website. Sample size available.',
'modified' => '2024-11-19 16:58:38',
'created' => '2016-02-17 16:33:58'
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'ProductsApplication' => array(
'id' => '4675',
'product_id' => '2901',
'application_id' => '36'
)
)
$slugs = array(
(int) 0 => 'rip'
)
$applications = array(
'id' => '36',
'position' => '10',
'parent_id' => '40',
'name' => 'RIP',
'description' => '<p>RIP</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'rip',
'meta_keywords' => 'RIP antibodies,classic monoclonal antibodies,Ago (Argonautes) monoclonal antibody,Snf2h monoclonal antibody - Classic,Diagenode',
'meta_description' => 'Diagenode offers a wide range of classic monoclonal RIP antibodies.',
'meta_title' => 'RIP antibodies | Diagenode',
'modified' => '2016-01-21 08:09:31',
'created' => '2015-07-08 13:54:45',
'locale' => 'jpn'
)
$description = '<p>RIP</p>'
$name = 'RIP'
$document = array(
'id' => '885',
'name' => 'm6A monoclonal antibody C15200082',
'description' => '<p>Monoclonal antibody raised in mouse against N6-methyladenosine (m6A) conjugated to BSA.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/C15200082_m6A-monoclonal-antibody.pdf',
'slug' => 'c15200082-m6a-monoclonal-antibody-pdf',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2017-04-19 16:18:17',
'created' => '2016-02-19 14:28:31',
'ProductsDocument' => array(
'id' => '2380',
'product_id' => '2901',
'document_id' => '885'
)
)
$sds = array(
'id' => '1404',
'name' => 'N6 methyladenosine(m6A) Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/N6/SDS-C15200082-N6-methyladenosine_m6A_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2021-08-30 13:16:13',
'created' => '2021-08-30 13:16:13',
'ProductsSafetySheet' => array(
'id' => '2462',
'product_id' => '2901',
'safety_sheet_id' => '1404'
)
)
$publication = array(
'id' => '4406',
'name' => 'Evaluation of N<sup>6</sup>-adenine DNA-immunoprecipitation-basedgenomic profiling in eukaryotes',
'authors' => 'Debo Brian M. et al.',
'description' => '<p>The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.</p>',
'date' => '2022-03-01',
'pmid' => 'https://doi.org/10.1101%2F2022.03.02.482749',
'doi' => '10.1101/2022.03.02.482749',
'modified' => '2022-08-11 15:02:40',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
'id' => '6071',
'product_id' => '2901',
'publication_id' => '4406'
)
)
$externalLink = ' <a href="https://doi.org/10.1101%2F2022.03.02.482749" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×