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N6-methyladenosine (m6A) polyclonal antibody

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ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15410208

50 μg

$380.00
Bulk order

Other format

C15410208-10

10 μg

Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.

Lot	A2125-0010
Concentration	1.2 µg/µl
Species reactivity	Human, mouse, other (wide range): positive.
Type	Polyclonal
Purity	Protein G purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	PBS containing 0.05% azide and 0.05% ProClin 300.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
RIP/RIP-seq*	1-2 µg per IP	Fig 1, 2, 3
Dot Blotting	1:400	Fig 4

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.

Validation Data

Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A
RNA Immunoprecipitation was performed on 40 µg HeLa total RNA spiked with 0.5 µg of an in vitro prepared transcript containing m6A nucleotides, using 2 µg of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A
RNA Immunoprecipitation was performed on 40 µg HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 µg of antibody per RIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3’ UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.
Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

A.

B.

C.

D.

Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A
RIP was performed with 2 µg of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).

Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A
To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.
実験手法
DB
Dot blotting Read more
RIP
RIP Read more

Safety sheets

SDS C15410208 N6 methyladenosine m6A Antibody US en	Download
SDS C15410208 N6 methyladenosine m6A Antibody GB en	Download
SDS C15410208 N6 methyladenosine m6A Antibody JP ja	Download
SDS C15410208 N6 methyladenosine m6A Antibody DE de	Download
SDS C15410208 N6 methyladenosine m6A Antibody ES es	Download
SDS C15410208 N6 methyladenosine m6A Antibody BE fr	Download
SDS C15410208 N6 methyladenosine m6A Antibody FR fr	Download
SDS C15410208 N6 methyladenosine m6A Antibody BE nl	Download

出版物

How to properly cite this product in your work

Diagenode strongly recommends using this: N6-methyladenosine (m6A) polyclonal antibody (Diagenode Cat# C15410208 Lot# A2125-0010). Click here to copy to clipboard.

Using our products in your publication? Let us know!

Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.
Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y
AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral bloo...

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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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<td>Fig 1, 2, 3</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &micro;g per IP.</small></p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p>
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p>
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<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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<td>Fig 1, 2, 3</td>
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<td>Dot Blotting</td>
<td>1:400</td>
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p>
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<p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p>
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<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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			'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>',
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	'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y',
	'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>',
	'date' => '2019-03-01',
	'pmid' => 'http://www.pubmed.gov/30744524',
	'doi' => '10.1177/0961203319828520',
	'modified' => '2019-06-28 13:53:56',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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	'meta_description' => 'To demonstrate the specificity of the Diagenode antibody against N6-methyladenosine (m6A), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on th',
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			'name' => 'N6-methyladenosine (m6A) polyclonal antibody ',
			'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p>
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p>
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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p>
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<p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p>
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<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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			'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
			'clonality' => '',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>RIP/RIP-seq*</td>
<td>1-2 &micro;g per IP</td>
<td>Fig 1, 2, 3</td>
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<td>Dot Blotting</td>
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<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &micro;g per IP.</small></p>',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p>
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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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		'slug' => 'm6a-polyclonal-antibody-pioneer-50-mg',
		'meta_title' => 'N6-methyladenosine (m6A) polyclonal antibody - Pioneer | Diagenode',
		'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ',
		'meta_description' => 'To demonstrate the specificity of the Diagenode antibody against N6-methyladenosine (m6A), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on th',
		'modified' => '2024-11-19 16:59:42',
		'created' => '2015-06-29 14:08:20',
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	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '315',
		'name' => 'm6A polyclonal antibody',
		'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A2125-0010',
		'concentration' => '1.2 &micro;g/&micro;l',
		'reactivity' => 'Human, mouse, other (wide range): positive.',
		'type' => 'Polyclonal',
		'purity' => 'Protein G purified polyclonal antibody.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>RIP/RIP-seq*</td>
<td>1-2 &micro;g per IP</td>
<td>Fig 1, 2, 3</td>
</tr>
<tr>
<td>Dot Blotting</td>
<td>1:400</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'PBS containing 0.05% azide and 0.05% ProClin 300.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2020-02-26 10:52:35',
		'created' => '0000-00-00 00:00:00',
		'select_label' => '315 - m6A polyclonal antibody (A2125-0010 - 1.2 &micro;g/&micro;l - Human, mouse, other (wide range): positive. - Protein G purified polyclonal antibody. - Rabbit)'
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			'name' => 'N6-methyladenosine (m6A) Antibody',
			'description' => '<p><span>Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.</span></p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">B. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-b.jpg" alt="RNA immunoprecipitation " /></p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-Fig1-DotBlot.jpg" alt="Dot blot" width="400" height="134" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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			'meta_description' => 'N6-methyladenosine (m6A) Polyclonal Antibody validated  in RIP and DB. Batch-specific data available on the website. Sample size available.',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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			'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y',
			'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>',
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	'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y',
	'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>',
	'date' => '2019-03-01',
	'pmid' => 'http://www.pubmed.gov/30744524',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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	'meta_keywords' => 'N6-methyladenosine (m6A),polyclonal antibody ',
	'meta_description' => 'To demonstrate the specificity of the Diagenode antibody against N6-methyladenosine (m6A), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on th',
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<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RIP.jpg" alt="RIP" /></p>
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410208-RNA.jpg" alt="RNA immunoprecipitation " /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p class="text-center">A. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-a.jpg" alt="RNA immunoprecipitation " /></p>
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<p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p>
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<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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			'description' => 'N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.
Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.',
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<td>Fig 1, 2, 3</td>
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p class="text-center">C. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-c.jpg" alt="RNA immunoprecipitation " /></p>
<p class="text-center">D. <img src="https://www.diagenode.com/img/product/antibodies/C15410208-ripseq-d.jpg" alt="RNA immunoprecipitation " /></p>
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<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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<td>Fig 1, 2, 3</td>
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<td>Fig 4</td>
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<p><small><strong>Figure 1. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA spiked with 0.5 &micro;g of an in vitro prepared transcript containing m6A nucleotides, using 2 &micro;g of the Diagenode m6A antibody (Cat. No. C15410208). An equal amount of IgG was used as negative control. The immunoprecipitated RNA was subsequently analyzed by qRT-PCR with primers specific for the transcript and for an intergenic region, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. RNA immunoprecipitation using the Diagenode antibody directed against m6A</strong><br />RNA Immunoprecipitation was performed on 40 &micro;g HeLa total RNA fragmented to a mean size of ~500 bases. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per RIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative RT-PCR was performed with primers for 3&rsquo; UTR of the C1RL and SLC35E1 genes, and for exon 12 of the AFF1 gene, used as positive controls, and for exon 5 of the EIF2S3 gene, used as negative control.<br /> Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-12 columns">
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 3. RIP-seq results obtained with the Diagenode antibody directed against m6A</strong><br />RIP was performed with 2 &micro;g of the Diagenode antibody against m6A (Cat. No. C15410208). The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 3 shows the signal distribution along the complete sequence of the human X-chromosome (figure 3A) and in three genomic regions surrounding the C1RL, SLC35E1 and AFF1 positive control genes (figure 3B, C and D).</small></p>
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<p><small><strong>Figure 2. Dot blot analysis using the Diagenode antibody directed against m6A</strong><br />To demonstrate the specificity of the Diagenode antibody against m6A (Cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo&rsquo;s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.</small></p>
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			'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>',
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	'locale' => 'jpn'
)
$description = '<p>RIP</p>'
$name = 'RIP'
$document = array(
	'id' => '38',
	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
	'slug' => 'epigenetic-antibodies-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2016-06-15 11:24:06',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '1856',
		'product_id' => '2286',
		'document_id' => '38'
	)
)
$sds = array(
	'id' => '3633',
	'name' => 'SDS C15410208 N6 methyladenosine m6A Antibody BE nl',
	'language' => 'nl',
	'url' => 'files/SDS/m6A/SDS-C15410208-N6-methyladenosine_m6A_Antibody-BE-nl-GHS_3_0.pdf',
	'countries' => 'BE',
	'modified' => '2024-01-17 17:02:45',
	'created' => '2024-01-17 17:02:45',
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		'id' => '5933',
		'product_id' => '2286',
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$publication = array(
	'id' => '3689',
	'name' => 'Genomewide analysis of 6-methyladenine DNA in peripheral blood mononuclear cells of systemic lupus erythematosus.',
	'authors' => 'Zheng F, Tang D, Xu H, Xu Y, Dai W, Zhang X, Hong X, Liu D, Dai Y',
	'description' => '<p>AIM: The aim of this paper is to explore the expression of 6-methyladenine (6mA) DNA and to elucidate its gene regulation role in systemic lupus erythematosus (SLE). METHODS: Twenty SLE patients and 20 normal control healthy individuals (HCs) were included in this study. Genomic DNA was isolated from peripheral blood mononuclear cells and subsequently underwent 6mA-immunoprecipitation-sequencing (6mA-IP-Seq) after DNA quality control and 6mA precipitation. Bioinformation analysis was applied to the raw data comparing 6mA levels between SLE patients and HCs. RESULTS: We identified 5462 hypermethylation and 431 hypomethylation genes in PBMCs of individuals with SLE, which indicated that a high level of 6mA participates in the pathogenesis of SLE. Gene ontology analysis revealed that hypermethylation genes might regulate the inflammatory process, which has been well documented in the pathogenesis of SLE. CONCLUSION: 6mA may be involved in the initial development of SLE, which may lead to its potential use as an early diagnostic marker and therapeutic target.</p>',
	'date' => '2019-03-01',
	'pmid' => 'http://www.pubmed.gov/30744524',
	'doi' => '10.1177/0961203319828520',
	'modified' => '2019-06-28 13:53:56',
	'created' => '2019-06-21 14:55:31',
	'ProductsPublication' => array(
		'id' => '3743',
		'product_id' => '2286',
		'publication_id' => '3689'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/30744524" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet m6A C15410208 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download