| Maize B73 inner stem ZmCopia Primer pair (C17040005) DATASHEET Datasheet | Download |
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction with the Universal Plant ChIP-seq kit and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP.
| Maize B73 inner stem ZmCopia Primer pair (C17040005) DATASHEET Datasheet | Download |
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Synthetic maize centromeres transmit chromosomes across generations. |
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Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Poplar</h4> <p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p> <div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <h4 class="text-center">Tomato</h4> <p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p> <div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Maize</h4> <p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p> <div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <p><strong> </strong></p> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> </tbody> </table> <p><strong></strong></p> <p><strong></strong></p>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '24 rxns', 'catalog_number' => 'C01010152', 'old_catalog_number' => '', 'sf_code' => 'C01010152-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '575', 'price_GBP' => '525', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns', 'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar', 'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody', 'modified' => '2023-04-20 16:15:50', 'created' => '2016-02-22 15:08:28', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2790', 'antibody_id' => null, 'name' => 'Auto Universal Plant ChIP-seq kit', 'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>', 'label1' => 'Characteristics', 'info1' => '<p></p> <ul> <li><strong>Universal compatiblity</strong> with a wide variety of plant species</li> <li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li> <li>Validated for the high throughput <strong>IP-Star® Automated System</strong></li> </ul> <h3>Successful ChIP-seq experiments for a variety of plants</h3> <div class="row"> <div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center> <p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> </tbody> </table>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '24 rxns', 'catalog_number' => 'C01010153', 'old_catalog_number' => '', 'sf_code' => 'C01010153-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '575', 'price_GBP' => '525', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-universal-plant-chip-seq-kit-x24-24-rxns', 'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star', 'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation', 'modified' => '2017-08-19 10:28:51', 'created' => '2016-02-22 15:08:26', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '9', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-seq', 'description' => '<div class="row"> <div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div> <div class="large-12 columns"></div> <h5 class="large-12 columns"><strong></strong></h5> <h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div> <div class="large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: Reverse cross-links, elute, and purify </li> <li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li> <li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chromatin-immunoprecipitation-sequencing', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers wide range of kits and antibodies for Chromatin Immunoprecipitation Sequencing (ChIP-Seq) and also provides Bioruptor for chromatin shearing', 'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation | Diagenode', 'modified' => '2017-11-14 09:57:16', 'created' => '2015-04-12 18:08:46', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns text-justify"> <p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. 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aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2791" id="CartAdd/2791Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2791" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Universal Plant ChIP-seq kit', 'C01010152', '575', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Universal Plant ChIP-seq kit', 'C01010152', '575', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="universal-plant-chip-seq-kit-x24-24-rxns" data-reveal-id="cartModal-2791" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Universal Plant ChIP-seq kit</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/auto-universal-plant-chip-seq-kit-x24-24-rxns"><img src="/img/product/kits/chip-kit-icon.png" alt="ChIP kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C01010153</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2790" id="CartAdd/2790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2790" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" 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style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. 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Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - 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R. et al.', 'description' => '<p>Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a tethering approach that activates functional maize centromeres at synthetic sequence arrays. A LexA-CENH3 fusion protein was used to recruit native Centromeric Histone H3 (CENH3) to long arrays of LexO repeats on a chromosome arm. Newly recruited CENH3 was sufficient to organize functional kinetochores that caused chromosome breakage, releasing chromosome fragments that were passed through meiosis and into progeny. Several fragments formed independent neochromosomes with centromeres localized over the LexO repeat arrays. The new centromeres were self-sustaining and transmitted neochromosomes to subsequent generations in the absence of the LexA-CENH3 activator. 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Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Poplar</h4> <p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p> <div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <h4 class="text-center">Tomato</h4> <p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p> <div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Maize</h4> <p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p> <div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <p><strong> </strong></p> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> </tbody> </table> <p><strong></strong></p> <p><strong></strong></p>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '24 rxns', 'catalog_number' => 'C01010152', 'old_catalog_number' => '', 'sf_code' => 'C01010152-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '575', 'price_GBP' => '525', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns', 'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar', 'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody', 'modified' => '2023-04-20 16:15:50', 'created' => '2016-02-22 15:08:28', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2790', 'antibody_id' => null, 'name' => 'Auto Universal Plant ChIP-seq kit', 'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>', 'label1' => 'Characteristics', 'info1' => '<p></p> <ul> <li><strong>Universal compatiblity</strong> with a wide variety of plant species</li> <li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li> <li>Validated for the high throughput <strong>IP-Star® Automated System</strong></li> </ul> <h3>Successful ChIP-seq experiments for a variety of plants</h3> <div class="row"> <div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center> <p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div> <div class="large-12 columns"></div> <h5 class="large-12 columns"><strong></strong></h5> <h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div> <div class="large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: Reverse cross-links, elute, and purify </li> <li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li> <li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chromatin-immunoprecipitation-sequencing', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers wide range of kits and antibodies for Chromatin Immunoprecipitation Sequencing (ChIP-Seq) and also provides Bioruptor for chromatin shearing', 'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation | Diagenode', 'modified' => '2017-11-14 09:57:16', 'created' => '2015-04-12 18:08:46', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns text-justify"> <p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. 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Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.</p>', 'date' => '2023-03-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36928774', 'doi' => '10.1038/s41477-023-01370-8', 'modified' => '2023-04-17 09:38:23', 'created' => '2023-04-14 13:41:22', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/jp/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = true $other_formats = array( (int) 0 => array( 'id' => '2794', 'antibody_id' => null, 'name' => 'Maize B73 inner stem ZmCopia primer pair', 'description' => '<p style="text-align: justify;">This <strong>primer pair</strong> specifically amplifies a genomic region in the Copia gene from <strong>Maize</strong> (<em>Zea mays</em> clone B73). 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aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2791" id="CartAdd/2791Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2791" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Universal Plant ChIP-seq kit', 'C01010152', '575', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" 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columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2790" id="CartAdd/2790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2790" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" 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style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>', 'label1' => 'Characteristics', 'info1' => '<p></p> <ul> <li><strong>Universal compatiblity</strong> with a wide variety of plant species</li> <li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li> <li>Validated for the high throughput <strong>IP-Star® Automated System</strong></li> </ul> <h3>Successful ChIP-seq experiments for a variety of plants</h3> <div class="row"> <div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center> <p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. 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Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. 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Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - 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Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Poplar</h4> <p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p> <div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <h4 class="text-center">Tomato</h4> <p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p> <div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Maize</h4> <p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p> <div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <p><strong> </strong></p> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> </tbody> </table> <p><strong></strong></p> <p><strong></strong></p>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '24 rxns', 'catalog_number' => 'C01010152', 'old_catalog_number' => '', 'sf_code' => 'C01010152-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '575', 'price_GBP' => '525', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns', 'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar', 'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody', 'modified' => '2023-04-20 16:15:50', 'created' => '2016-02-22 15:08:28', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2790', 'antibody_id' => null, 'name' => 'Auto Universal Plant ChIP-seq kit', 'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. 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Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div> <div class="large-12 columns"></div> <h5 class="large-12 columns"><strong></strong></h5> <h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div> <div class="large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: Reverse cross-links, elute, and purify </li> <li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li> <li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chromatin-immunoprecipitation-sequencing', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers wide range of kits and antibodies for Chromatin Immunoprecipitation Sequencing (ChIP-Seq) and also provides Bioruptor for chromatin shearing', 'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation | Diagenode', 'modified' => '2017-11-14 09:57:16', 'created' => '2015-04-12 18:08:46', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns text-justify"> <p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. 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With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. 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These Primers are optimized for routine SYBR® Green Real-Time qPCR assay following ChIP', 'meta_title' => 'Primer pairs - Plant | Diagenode', 'modified' => '2016-03-02 08:40:16', 'created' => '2015-09-01 14:03:44', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '889', 'name' => 'Maize B73 inner stem ZmCopia Primer pair (C17040005)', 'description' => '<p>Datasheet</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/C17040005-maize-b73-inner-stem-zmcopia.pdf', 'slug' => 'maize-b73-inner-stem-zmcopia-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-01 11:44:00', 'created' => '2016-04-01 11:30:46', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4770', 'name' => 'Synthetic maize centromeres transmit chromosomes across generations.', 'authors' => 'Kelly D. R. et al.', 'description' => '<p>Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a tethering approach that activates functional maize centromeres at synthetic sequence arrays. A LexA-CENH3 fusion protein was used to recruit native Centromeric Histone H3 (CENH3) to long arrays of LexO repeats on a chromosome arm. Newly recruited CENH3 was sufficient to organize functional kinetochores that caused chromosome breakage, releasing chromosome fragments that were passed through meiosis and into progeny. Several fragments formed independent neochromosomes with centromeres localized over the LexO repeat arrays. The new centromeres were self-sustaining and transmitted neochromosomes to subsequent generations in the absence of the LexA-CENH3 activator. 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aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2791" id="CartAdd/2791Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2791" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Universal Plant ChIP-seq kit', 'C01010152', '575', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" 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Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - 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R. et al.', 'description' => '<p>Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a tethering approach that activates functional maize centromeres at synthetic sequence arrays. A LexA-CENH3 fusion protein was used to recruit native Centromeric Histone H3 (CENH3) to long arrays of LexO repeats on a chromosome arm. Newly recruited CENH3 was sufficient to organize functional kinetochores that caused chromosome breakage, releasing chromosome fragments that were passed through meiosis and into progeny. Several fragments formed independent neochromosomes with centromeres localized over the LexO repeat arrays. The new centromeres were self-sustaining and transmitted neochromosomes to subsequent generations in the absence of the LexA-CENH3 activator. Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.</p>', 'date' => '2023-03-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36928774', 'doi' => '10.1038/s41477-023-01370-8', 'modified' => '2023-04-17 09:38:23', 'created' => '2023-04-14 13:41:22', 'ProductsPublication' => array( 'id' => '6843', 'product_id' => '2795', 'publication_id' => '4770' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36928774" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Poplar</h4> <p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p> <div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <h4 class="text-center">Tomato</h4> <p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p> <div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"> <h4 class="text-center">Maize</h4> <p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p> <div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p> <a class="close-reveal-modal" aria-label="Close">×</a></div> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <p><strong> </strong></p> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> </tbody> </table> <p><strong></strong></p> <p><strong></strong></p>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '24 rxns', 'catalog_number' => 'C01010152', 'old_catalog_number' => '', 'sf_code' => 'C01010152-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '575', 'price_GBP' => '525', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns', 'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar', 'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody', 'modified' => '2023-04-20 16:15:50', 'created' => '2016-02-22 15:08:28', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2790', 'antibody_id' => null, 'name' => 'Auto Universal Plant ChIP-seq kit', 'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>', 'label1' => 'Characteristics', 'info1' => '<p></p> <ul> <li><strong>Universal compatiblity</strong> with a wide variety of plant species</li> <li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li> <li>Validated for the high throughput <strong>IP-Star® Automated System</strong></li> </ul> <h3>Successful ChIP-seq experiments for a variety of plants</h3> <div class="row"> <div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center> <p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p> </div> <div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center> <p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> </tbody> </table>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '24 rxns', 'catalog_number' => 'C01010153', 'old_catalog_number' => '', 'sf_code' => 'C01010153-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '575', 'price_GBP' => '525', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1438', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-universal-plant-chip-seq-kit-x24-24-rxns', 'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star', 'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation', 'modified' => '2017-08-19 10:28:51', 'created' => '2016-02-22 15:08:26', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '9', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-seq', 'description' => '<div class="row"> <div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div> <div class="large-12 columns"></div> <h5 class="large-12 columns"><strong></strong></h5> <h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div> <div class="large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: Reverse cross-links, elute, and purify </li> <li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li> <li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chromatin-immunoprecipitation-sequencing', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers wide range of kits and antibodies for Chromatin Immunoprecipitation Sequencing (ChIP-Seq) and also provides Bioruptor for chromatin shearing', 'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation | Diagenode', 'modified' => '2017-11-14 09:57:16', 'created' => '2015-04-12 18:08:46', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns text-justify"> <p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. 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aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2791" id="CartAdd/2791Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2791" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Universal Plant ChIP-seq kit', 'C01010152', '575', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Universal Plant ChIP-seq kit', 'C01010152', '575', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="universal-plant-chip-seq-kit-x24-24-rxns" data-reveal-id="cartModal-2791" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Universal Plant ChIP-seq kit</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/auto-universal-plant-chip-seq-kit-x24-24-rxns"><img src="/img/product/kits/chip-kit-icon.png" alt="ChIP kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C01010153</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2790" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/2790" id="CartAdd/2790Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2790" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Auto Universal Plant ChIP-seq kit個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" 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style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star® automated system</strong>. 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Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center> <p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p> </div> <div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center> <p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation™ kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p> </div> </div> <table style="width: 856px;"> <tbody> <tr> <td style="width: 224px;"> <h4><strong>Plant Species</strong></h4> </td> <td style="width: 341px;"> <h4><strong>Validated antibodies</strong></h4> </td> <td style="width: 357px;"> <h4><strong>Validated primer pairs</strong></h4> </td> </tr> <tr> <td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td> <td style="width: 357px;"></td> </tr> <tr> <td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td> <td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td> </tr> <tr> <td style="width: 224px;"></td> <td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - 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The primers are thoroughly tested in conjunction with the Universal Plant ChIP-seq kit and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP.</p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17040005-50', 'old_catalog_number' => '', 'sf_code' => 'C17040005-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'Maize-B73-inner-stem-ZmCopia-primer-pair-50ul', 'meta_title' => 'Maize B73 inner stem ZmCopia primer pair | Diagenode', 'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Maize B73 inner stem ZmCopia primer pair', 'meta_description' => 'Primer pair specific for region in the Copia gene from Maize (Zea mays clone B73). Validated in conjunction with the Universal Plant ChIP-seq kit', 'modified' => '2017-11-29 13:59:30', 'created' => '2016-03-17 21:30:09' ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '889', 'name' => 'Maize B73 inner stem ZmCopia Primer pair (C17040005)', 'description' => '<p>Datasheet</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/C17040005-maize-b73-inner-stem-zmcopia.pdf', 'slug' => 'maize-b73-inner-stem-zmcopia-datasheet', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-01 11:44:00', 'created' => '2016-04-01 11:30:46', 'ProductsDocument' => array( 'id' => '2001', 'product_id' => '2795', 'document_id' => '889' ) ) $publication = array( 'id' => '4770', 'name' => 'Synthetic maize centromeres transmit chromosomes across generations.', 'authors' => 'Kelly D. R. et al.', 'description' => '<p>Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a tethering approach that activates functional maize centromeres at synthetic sequence arrays. A LexA-CENH3 fusion protein was used to recruit native Centromeric Histone H3 (CENH3) to long arrays of LexO repeats on a chromosome arm. Newly recruited CENH3 was sufficient to organize functional kinetochores that caused chromosome breakage, releasing chromosome fragments that were passed through meiosis and into progeny. Several fragments formed independent neochromosomes with centromeres localized over the LexO repeat arrays. The new centromeres were self-sustaining and transmitted neochromosomes to subsequent generations in the absence of the LexA-CENH3 activator. Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.</p>', 'date' => '2023-03-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36928774', 'doi' => '10.1038/s41477-023-01370-8', 'modified' => '2023-04-17 09:38:23', 'created' => '2023-04-14 13:41:22', 'ProductsPublication' => array( 'id' => '6843', 'product_id' => '2795', 'publication_id' => '4770' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36928774" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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