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Alternative names: CXXC3, PCM1, RFT
Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein.
| Lot | A1223P |
|---|---|
| Concentration | 0.6 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:1,000 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1
HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. No. C15410038) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet MBD1 pAb-038-050 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: MBD1 polyclonal antibody (Diagenode Cat# C15410038 Lot# A1223P). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD1 polyclonal antibody - Pioneer', 'meta_title' => 'MBD1 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2203', 'antibody_id' => '267', 'name' => 'MBD1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>CXXC3</strong>, <strong>PCM1</strong>, <strong>RFT</strong></p> <p>Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein. </p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410038-ELISA.png" alt="ELISA" height="308" width="340" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD1 polyclonal antibody - Pioneer', 'meta_title' => 'MBD1 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2203', 'antibody_id' => '267', 'name' => 'MBD1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>CXXC3</strong>, <strong>PCM1</strong>, <strong>RFT</strong></p> <p>Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein. </p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410038-ELISA.png" alt="ELISA" height="308" width="340" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD1 polyclonal antibody - Pioneer', 'meta_title' => 'MBD1 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2203', 'antibody_id' => '267', 'name' => 'MBD1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>CXXC3</strong>, <strong>PCM1</strong>, <strong>RFT</strong></p> <p>Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein. </p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410038-ELISA.png" alt="ELISA" height="308" width="340" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. 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Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD1 polyclonal antibody - Pioneer', 'meta_title' => 'MBD1 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2203', 'antibody_id' => '267', 'name' => 'MBD1 polyclonal antibody ', 'description' => '<p>Alternative names: <strong>CXXC3</strong>, <strong>PCM1</strong>, <strong>RFT</strong></p> <p>Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein. </p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410038-ELISA.png" alt="ELISA" height="308" width="340" /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD1 (cat. No. C15410038) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000. </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410038-WB.jpg" alt="Western blot" height="168" width="172" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD1</strong><br />HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against MBD1 (cat. No. C15410038) diluted 1:1,000 in TBST containing 5% milk powder. 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