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NRF1 monoclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15200013

50 μg

$380.00
Bulk order

Other format

C15200013-10

10 µg

Monoclonal antibody raised in mouse against human NRF1 (Nuclear Respiratory Factor 1), using a recombinant protein.

Lot	001
Concentration	2.2 μg/μl
Species reactivity	Human
Type	Monoclonal
Purity	Protein A purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	2 μg/ChIP	Fig 1, 2
Western Blotting	1:1,000	Fig 3

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1
ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1
ChIP was performed with 2 μg of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).

Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1
Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target Description
NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.

資料
NRF1 monoclonal antibody DATASHEET
Datasheet of the NRF1 monoclonal antibody Download

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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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$country_code = 'US'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<li>100% satisfaction guarantee</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<td>Fig 1, 2</td>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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		'slug' => 'nrf1-monoclonal-antibody-classic',
		'meta_title' => 'NRF1 monoclonal antibody - Classic | Diagenode',
		'meta_keywords' => 'Monoclonal antibody,NRF1 monoclonal antibody,ChIP-seq',
		'meta_description' => 'Monoclonal antibody raised in mouse against human NRF1 (Nuclear Respiratory Factor 1), using a recombinant protein. ',
		'modified' => '2021-12-23 12:23:07',
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	'Antibody' => array(
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		'id' => '466',
		'name' => 'NRF1 monoclonal antibody - Classic',
		'description' => 'NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '2.2 μg/μl',
		'reactivity' => 'Human',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &mu;g per IP.</small></p>',
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		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'name' => 'NRF1 Antibody',
			'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein.&nbsp;</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p>
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<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
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ReflectionMethod::invokeArgs() - [internal], line ??
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.</p>',
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		'catalog_number' => 'C15200013',
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		'sf_code' => 'C15200013-D001-000581',
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		'slug' => 'nrf1-monoclonal-antibody-classic',
		'meta_title' => 'NRF1 monoclonal antibody - Classic | Diagenode',
		'meta_keywords' => 'Monoclonal antibody,NRF1 monoclonal antibody,ChIP-seq',
		'meta_description' => 'Monoclonal antibody raised in mouse against human NRF1 (Nuclear Respiratory Factor 1), using a recombinant protein. ',
		'modified' => '2021-12-23 12:23:07',
		'created' => '2015-12-02 15:28:00',
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	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '466',
		'name' => 'NRF1 monoclonal antibody - Classic',
		'description' => 'NRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 has also been associated with the regulation of neurite outgrowth.',
		'clonality' => '',
		'isotype' => '',
		'lot' => '001',
		'concentration' => '2.2 μg/μl',
		'reactivity' => 'Human',
		'type' => 'Monoclonal',
		'purity' => 'Protein A purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &mu;g per IP.</small></p>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'modified' => '2019-09-09 16:49:58',
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			'description' => '<p>Monoclonal antibody raised in mouse against human <strong>NRF1 (Nuclear Respiratory Factor 1)</strong>, using a recombinant protein.&nbsp;</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIP.jpg" alt="NRF1 Antibody ChIP Grade" caption="false" width="288" height="219" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 &mu;g per ChIP experiment was analysed. IgG (2 &mu;g/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-A.jpg" alt="NRF1 Antibody ChIP-seq Grade" caption="false" width="447" height="53" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-B.jpg" alt="NRF1 Antibody for ChIP-seq " caption="false" width="447" height="110" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-C.jpg" alt="NRF1 Antibody for ChIP-seq assay" caption="false" width="447" height="93" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-ChIPseq-D.jpg" alt="NRF1 Antibody validated in ChIP-seq" caption="false" width="447" height="94" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against NRF1</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against NRF1 (Cat. No. C15200013) on sheared chromatin from 4 million K562 cells as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 (figure 2A and B) and in two regions surrounding the BRCA1 and AURKA positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200013-WB.jpg" alt="NRF1 Antibody validated in Western Blot " caption="false" width="200" height="277" /></p>
</div>
<div class="small-8 columns">
<p><small> <strong>Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against NRF1</strong> <br /> Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against NRF1 (Cat. No. C15200013) diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
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			'slug' => 'chip-seq-antibodies',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
			'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
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			'name' => 'Transcription',
			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies',
			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
			'meta_title' => 'Transcription factor Antibodies | Diagenode',
			'modified' => '2020-07-06 12:59:19',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-nl-GHS_2_0.pdf',
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			'language' => 'fr',
			'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-BE-fr-GHS_2_0.pdf',
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			'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-ES-es-GHS_2_0.pdf',
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	(int) 7 => 'FI',
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	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
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		'price_GBP' => '100',
		'price_JPY' => '16450',
		'price_CNY' => '0',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => '',
		'slug' => 'nrf1-monoclonal-antibody-classic-10-ug',
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		'meta_keywords' => '',
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		'created' => '2018-01-08 12:47:58',
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	'description' => '',
	'label1' => 'Validation data',
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$labelize = object(Closure) {
	
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	'tabular' => true,
	'slug' => 'chip-seq-antibodies',
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	'created' => '2015-10-20 11:44:45',
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	'meta_description' => '',
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		'document_id' => '861'
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	'name' => 'NRF1 antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/NRF1/SDS-C15200013-NRF1_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-08-27 15:06:59',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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NRF1 monoclonal antibody

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