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PADI4 polyclonal antibody (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15310075-20

20 µl

$115.00
Bulk order

Other format

C15310075

100 µl

Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5

Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.

Lot	A63-001
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:1,000 - 1:5,000	Fig 1
Western Blotting	1:1,000	Fig 2

Validation data

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.

Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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	'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig1.jpg" alt="ELISA" height="234" width="269" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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	'created' => '2015-06-29 14:08:20'
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$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<tr>
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<td>1:1,000 - 1:5,000</td>
<td>Fig 1</td>
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<td>Fig 2</td>
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</table>',
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<div class="small-5 columns">
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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<td>Fig 1</td>
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<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310075_fig2.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="157" width="89" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<p><span>Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the antibody titer<br /></strong> To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.<br /></small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4</strong> <br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 &mu;g) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Datasheet PADI4 CS-075-100 DATASHEET Datasheet description	Download

PADI4 polyclonal antibody (sample size)

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